抗GATA-3抗体(Anti-GATA-3, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human GATA-3Pro135-Ser258Accession # P23771
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human and mouse GATA-3 in direct ELISAs and Western blots.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Simple Western	2.5 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Immunocytochemistry
	GATA‑3 in MCF-7 Human Cell Line. GATA‑3 was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	GATA‑3 in Human Breast Cancer Tissue. GATA‑3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
	GATA‑3 in Human Breast Cancer Tissue. GATA‑3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western Blot
	Detection of Human and Mouse GATA‑3 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, and EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for GATA‑3 full length (FL) at approximately 55 kDa and the splice form (SF) at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western
	Detection of Human GATA‑3 by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. Specific bands were detected for GATA‑3 full length (FL) at approximately 62 kDa and the splice form (SF) at approximately 52 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human GATA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2605) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

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Related Product & Information

Entrez Gene IDs	2625 (Human); 14462 (Mouse)
Background	GATA-3
background_content	Background: GATA-3 GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development and the maintenance of the differentiated state in epithelial cells.

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Citations

1. GATA3 targets semaphorin 3B in mammary epithelial cells to suppress breast cancer progression and metastasis
   Authors: P Shahi, CY Wang, J Chou, C Hagerling, H Gonzalez V, A Ruderisch, Y Yu, MD Lai, Z Werb
   Oncogene, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis.
   Authors: Takasato M, Er P, Chiu H, Maier B, Baillie G, Ferguson C, Parton R, Wolvetang E, Roost M, Chuva de Sousa Lopes S, Little M
   Nature, 2015;526(7574):564-8.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Not Specified


3. Human cytomegalovirus infection interferes with the maintenance and differentiation of trophoblast progenitor cells of the human placenta.
   Authors: Tabata T, Petitt M, Zydek M, Fang-Hoover J, Larocque N, Tsuge M, Gormley M, Kauvar L, Pereira L
   J Virol, 2015;89(9):5134-47.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC Paraffin-embedded


4. A Gata3-Mafb transcriptional network directs post-synaptic differentiation in synapses specialized for hearing.
   Authors: Yu, Wei-Ming, Appler, Jessica, Kim, Ye-Hyun, Nishitani, Allison, Holt, Jeffrey, Goodrich, Lisa V
   Elife, 2013;2(0):e01341.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Cytokines inhibit norepinephrine transporter expression by decreasing Hand2.
   Authors: Pellegrino MJ, Parrish DC, Zigmond RE, Habecker BA
   Mol. Cell. Neurosci., 2011;46(3):671-80.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Critical role for GATA3 in mediating Tie2 expression and function in large vessel endothelial cells.
   Authors: Song H, Suehiro J, Kanki Y, Kawai Y, Inoue K, Daida H, Yano K, Ohhashi T, Oettgen P, Aird WC, Kodama T, Minami T
   J. Biol. Chem., 2009;284(42):29109-24.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. Novel immunomodulatory properties of berbamine through selective down-regulation of STAT4 and action of IFN-gamma in experimental autoimmune encephalomyelitis.
   Authors: Ren Y, Lu L, Guo TB, Qiu J, Yang Y, Liu A, Zhang JZ
   J. Immunol., 2008;181(2):1491-8.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB

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