抗HGF抗体(Anti-HGF, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse HGF (R&D Systems, Catalog # 2207-HG)Gln33-Leu728Accession # Q53WS5
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse HGF in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) HGF, rhHGF R, recombinant mouse (rm) HGF R, rhHGF-A, and rmHGF-A is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse HGF (Catalog # 2207-HG)
Immunohistochemistry	5-15 µg/mL	See below
Mouse HGF Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	0.2-0.8 µg/mL	Mouse HGF Antibody (Catalog #AF2207 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse HGF Biotinylated Antibody (Catalog #BAF2207 )
ELISA Standard		Recombinant Mouse HGF Protein (Catalog #2207-HG )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Immunohistochemistry	click image to view larger	HGF in Mouse Liver.HGF was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry	click image to view larger	HGF in Mouse Skeletal Muscle.HGF was detected in perfusion fixed frozen sections of mouse skeletal muscle using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HGF	HGF, also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates an isoform that lacks the peptidase and the second, third, and fourth Kringle domains. Mouse HGF shares 91%‑95% aa sequence identity with bovine, canine, feline, human, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

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References

Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
Sasaki, M. et al. (1994) Biochem. Biophys. Res. Commun. 199:772.
Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
Corso, S. et al. (2005) Trends Mol. Med. 11:284.
Maeshima, A. et al. (2000) Kid. Int. 58:1511.
Montesano, R. et al. (1991) Cell 67:901.
Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
Mineo, R. et al. (1994) Endocrinology 145:4355.
Urbanek, K. et al. (2005) Circ. Res. 97:663.
Long Name:	Hepatocyte Growth Factor
Entrez Gene IDs:	3082 (Human); 15234 (Mouse); 403441 (Canine)
Alternate Names:	deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A

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Related Research Areas

Show

Adhesion Molecules in Angiogenesis
Alpha Granule Soluble Molecules
Cytokines and Receptors in Angiogenesis
EMT Induction
Gametes and Fertilization
Hepatic Endoderm
Integrins and Associated Molecules
Pancreatic Endoderm
Preeclampsia
Semaphorins, Plexin Receptors, and Related Molecules
Progesterone dose-dependently modulates hepatocyte growth factor production in 3T3-L1 mouse preadipocytes Authors: T Ito, D Yamaji, A Kamikawa, MA Abd Eldaim, Y Okamatsu-O, A Terao, M Saito, K Kimura Endocr. J., 2017;0(0):. Species: Mouse Sample Type: Cell Lysates Application: WB
Images	Ratings	Applications	Species	Reviewed By	Date	Details
	Very Good		Immunocytochemistry/Immunoflourescence	Mouse	Anonymous	12/04/2015			Image Details	Immunocytochemistry/Immunoflourescence: Mouse HGF Antibody [AF2207].

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Summary

Application	Immunocytochemistry/Immunoflourescence
Sample Tested	Cultured cells and tissues
Species	Mouse
Other Experimental Details	We tested this antibody, mHGF on our cultured cells and skin tissue with immunofluorescent and immunohistochemical staining techniques. The results are very satisfactory. This mHGF antibody binds specifically to the epitope on cultured hematopoietic stem cells that we isolated from adult FVB mice. We like this antibody for its sensitivity and specificity. The provided small package provided a very good pattern of flexibility for users too.

Immunohistochemistry
	HGF in Mouse Liver. HGF was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
	HGF in Mouse Skeletal Muscle. HGF was detected in perfusion fixed frozen sections of mouse skeletal muscle using Goat Anti-Mouse HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2207) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background

HGF

background_content

Background: HGF HGF, also known as Scatter Factor and Hepatopoietin A, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. Alternate splicing generates an isoform that lacks the peptidase and the second, third, and fourth Kringle domains. Mouse HGF shares 91%‑95% aa sequence identity with bovine, canine, feline, human, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

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Citations

1. Progesterone dose-dependently modulates hepatocyte growth factor production in 3T3-L1 mouse preadipocytes
   Authors: T Ito, D Yamaji, A Kamikawa, MA Abd Eldaim, Y Okamatsu-O, A Terao, M Saito, K Kimura
   Endocr. J., 2017;0(0):.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


2. Epigenetic regulation of HGF/Met receptor axis is critical for the outgrowth of bone metastasis from breast carcinoma
   Authors: P Bendinelli, P Maroni, E Matteucci, MA Desiderio
   Cell Death Dis, 2017;8(2):e2578.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Targeting Serglycin Prevents Metastasis in Murine Mammary Carcinoma
   PLoS ONE, 2016;11(5):e0156151.
   Species: Mouse
   Sample Type: N/A
   Application: Not Specified


4. MET is required for the recruitment of anti-tumoural neutrophils.
   Authors: Finisguerra V, Di Conza G, Di Matteo M, Serneels J, Costa S, Thompson A, Wauters E, Walmsley S, Prenen H, Granot Z, Casazza A, Mazzone M
   Nature, 2015;522(7556):349-53.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
   Authors: Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R
   Cell Death Differ, 2015;22(7):1219-30.
   Species: Mouse
   Sample Type: Whole Cells
   Application: IHC Not Specified


6. Bone marrow progenitor cell therapy-mediated paracrine regulation of cardiac miRNA-155 modulates fibrotic response in diabetic hearts.
   Authors: Kishore, Raj, Verma, Suresh K, Mackie, Alexande, Vaughan, Erin E, Abramova, Tatiana, Aiko, Ito, Krishnamurthy, Prasanna
   PLoS ONE, 2013;8(4):e60161.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


7. Hepatocyte growth factor activator inhibitor type 1 is a suppressor of intestinal tumorigenesis.
   Authors: Hoshiko S, Kawaguchi M, Fukushima T, Haruyama Y, Yorita K, Tanaka H, Seiki M, Inatsu H, Kitamura K, Kataoka H
   Cancer Res, 2013;73(8):2659-70.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


8. Enhanced c-Met activity promotes G-CSF-induced mobilization of hematopoietic progenitor cells via ROS signaling.
   Authors: Tesio M, Golan K, Corso S
   Blood, 2011;117(2):419-28.
   Species: Mouse
   Sample Type: Plasma
   Application: ELISA Development


9. c-Met and its ligand hepatocyte growth factor/scatter factor regulate mature B cell survival in a pathway induced by CD74.
   Authors: Gordin M, Tesio M, Cohen S
   J. Immunol., 2010;185(4):2020-31.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


10. MCP-1 antibody treatment enhances damage and impedes repair of the alveolar epithelium in influenza pneumonitis.
    Authors: Narasaraju T, Ng HH, Phoon MC
    Am. J. Respir. Cell Mol. Biol., 2010;42(6):732-43.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

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