抗Mouse IL-15抗体(Anti-Mouse IL-15 antibody)
掲載日情報:2021/01/28 現在Webページ番号:30910
Mouse IL-15に対する抗体(Anti-Mouse IL-15 )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 :2025年04月26日 20時35分現在]
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Anti-Mouse IL-15 MAb (Clone 201136) |
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Anti-Mouse IL-15 MAb (Clone 201136) |
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Anti-Mouse IL-15 MAb (Clone 201136) |
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[在庫・価格 :2025年04月26日 20時35分現在]
Anti-Mouse IL-15 MAb (Clone 201136)
文献数: 1
- 商品コード:MAB447-100
- メーカー:RSD
- 包装:100μg
- 価格:¥100,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 別名:IL15 クローン:201136 Genbank No: 3600 Protein Accession No: P48346 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse IL-15 MAb (Clone 201136)
文献数: 1
- 商品コード:MAB447-500
- メーカー:RSD
- 包装:500μg
- 価格:¥232,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | マッチドペア:Mouse IL-15 サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF447,スタンダードとして#447-ML-010を用いる。 別名:IL15 クローン:201136 Genbank No: 3600 Protein Accession No: P48346 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
|
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製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse IL-15 MAb (Clone 201136)
文献数: 1
- 商品コード:MAB447-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | ※受注発注品。形状:溶液または凍結乾燥 別名:IL15 クローン:201136 Genbank No: 3600 Protein Accession No: P48346 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
|
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製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体/抗造血幹細胞マーカー抗体(R&D Systems社) 使いっきり抗体 |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Mouse |
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Label | Unconjugated |
Immunogen | E. coli-derived recombinant mouse IL-15Asn49-Ser162Accession # P48346 |
Source | Monoclonal Rat IgG2A Clone # 201136 |
Purification | Protein A or G purified from hybridoma culture supernatant |
Specificity | Detects mouse IL-15 in ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) IL‑2, rhIL‑15, or rmIL-21. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 1 µg/mL | Recombinant Mouse IL‑15 (Catalog # 447-ML) |
Mouse IL-15 Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse IL‑15 Antibody (Catalog #MAB447 ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse IL‑15 Biotinylated Antibody (Catalog #BAF447 ) |
ELISA Standard | Recombinant Mouse IL-15 Protein (Catalog #447-ML ) | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: IL-15 | Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1 - 3). Mature mouse IL-15 shares 70% and 96% amino acid sequence identity with human and rat IL-15, respectively. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11 - 13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15). |
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References
Grabstein, K. et al. (1994) Science 264:965. | |
Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259. | |
Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657. | |
Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. 94:14444. | |
Giri, J.G. et al. (1995) EMBO 14:3654. | |
Giri, J. et al. (1994) EMBO J. 13:2822. | |
Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851. | |
Dubois, S. et al. (2002) Immunity 17:537. | |
Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85. | |
Burkett, P.R. et al. (2004) J. Exp. Med. 200:825. | |
Budagian, V. et al. (2004) J. Biol. Chem. 279:40368. | |
Mortier, E. et al. (2004) J. Immunol. 173:1681. | |
Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167. | |
Budagian, V. et al. (2004) J. Biol. Chem. 279:42192. | |
Neely, G.G. et al. (2004) J. Immunol. 172:4225. | |
Long Name: | Interleukin 15 |
Entrez Gene IDs: | 3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline) |
Alternate Names: | IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15 |
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Related Research Areas
Common gamma Chain Receptor Family | ||
Group 1 ILCs | ||
Isolation, Culture, and Identification of Natural Killer Cells | ||
Macrophage Activation Markers | ||
Molecules Secreted by VSMC | ||
Natural Killer T (NKT) Cells | ||
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Related Product & Information
Long Name | Interleukin 15 |
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Entrez Gene IDs | 3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline) |
Background | IL-15 |
background_content | Background: IL-15 Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1 - 3). Mature mouse IL-15 shares 70% and 96% amino acid sequence identity with human and rat IL-15, respectively. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11 - 13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15). |
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Citations
- The Role of IL-15 in Activating STAT5 and Fine-Tuning IL-17A Production in CD4 T Lymphocytes.
Authors: Pandiyan P, Yang X, Saravanamuthu S, Zheng L, Ishihara S
J. Immunol., 2012;189(9):4237-46.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: ELISA Development - IL-12 rapidly alters the functional profile of tumor-associated and tumor-infiltrating macrophages in vitro and in vivo.
Authors: Watkins SK, Egilmez NK, Suttles J, Stout RD
J. Immunol., 2007;178(3):1357-62.
Species: Mouse
Sample Type: Serum
Application: ELISA Development
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