抗ST2抗体(Anti-ST2, Goat-Poly, Biotin antibody)

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• Product Details
• Applications and Data
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Biotin
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ST2/IL-33 RSer27-Ser342Accession # P14719
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse ST2/IL-33 R in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) IL-1 R4, recombinant mouse (rm) IL-1 R1, rmIL-2 R2, rhIL-1 R4, rmIL-1 R6, rmIL-1 R8, and rhIL-1 R9 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse ST2/IL-33 R Fc Chimera (Catalog # 1004-MR)
Mouse ST2/IL-33 R Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse ST2/IL-33 R Antibody (Catalog #MAB10041 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse ST2/IL-33 R Biotinylated Antibody (Catalog #BAF1004 )
ELISA Standard		Recombinant Mouse ST2/IL-33 R Fc Chimera Protein, CF (Catalog #1004-MR )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ST2/IL-33 R	ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses. Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain. Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions. Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes. It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts. IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL‑1 R rp2. In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal. In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy. The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma. Soluble ST2 functions as a decoy receptor that blocks IL-33’s ability to signal through transmembrane ST2.
Long Name:	Interleukin 33 Receptor
Entrez Gene IDs:	9173 (Human); 17082 (Mouse)
Alternate Names:	DER4; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33R; Ly84; ST2; ST2L; ST2V; suppression of tumorigenicity 2; T1

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Related Research Areas

Group 2 ILCs

IL-1 Family

Th2 Cells

The receptor tyrosine kinase c-Kit controls IL-33 receptor signaling in mast cells. Authors: Drube S, Heink S, Walter S, Lohn T, Grusser M, Gerbaulet A, Berod L, Schons J, Dudeck A, Freitag J, Grotha S, Reich D, Rudeschko O, Norgauer J, Hartmann K, Roers A, Kamradt T Blood, 2010;115(19):3899-906. Species: Human Sample Type: Cell Lysates Application: WB

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Related Product & Information

Background

ST2/IL-33 R

background_content

Background: ST2/IL-33 R ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses. Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain. Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions. Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes. It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts. IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL‑1 R rp2. In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal. In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy. The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma. Soluble ST2 functions as a decoy receptor that blocks IL-33’s ability to signal through transmembrane ST2.

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Citations

1. The receptor tyrosine kinase c-Kit controls IL-33 receptor signaling in mast cells.
   Authors: Drube S, Heink S, Walter S, Lohn T, Grusser M, Gerbaulet A, Berod L, Schons J, Dudeck A, Freitag J, Grotha S, Reich D, Rudeschko O, Norgauer J, Hartmann K, Roers A, Kamradt T
   Blood, 2010;115(19):3899-906.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB

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