抗NKp46/MAR-1抗体(Anti-NKp46/MAR-1, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse NKp46/NCR1Glu22-Asn255Accession # Q8C567
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse NKp46/NCR1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) NKp46 is observed and less than 1% cross-reactivity with rhNKp80 and rhNKp30 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse NKp46/NCR1 Fc Chimera (Catalog # 2225-NK)
Flow Cytometry	2.5 µg/106 cells	See below
Immunohistochemistry	3-15 µg/mL	See below
Agonist Activity	0.4-2.4 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Agonist Activity
	Mouse NKp46/NCR1 Antibody Induces IFN-gamma Secretion in Activated Mouse NK Cells. Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody induces IFN-gamma secretion in mouse natural killer (NK) cells activated with 25 ng/mL Recombinant Mouse IL-2 (Catalog # 402-ML) and 25 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML), in a dose-dependent manner, as measured using the Quantikine Mouse IFN-gamma ELISA Kit (Catalog # MIF00). The ED50 for this effect is typically0.4-2.4 μg/mL.
Flow Cytometry
	Detection of NKp46/NCR1 in Mouse DX5/CD49b+ Splenocytes by Flow Cytometry. Mouse DX5/CD49b+ splenocytes were stained with Goat Anti-Mouse NKp46/NCR1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2225, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Immunohistochemistry
	NKp46/NCR1 in Mouse Spleen. NKp46/NCR1 was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2225) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Related Product & Information

Entrez Gene IDs	9437 (Human); 17086 (Mouse)
Background	NKp46/NCR1
background_content	Background: NKp46/NCR1 NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. In human systems, the CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is upregulated by IL-2. Mouse NKp46, also known as MAR-1 (3), is a type I transmembrane protein with two extracellular Ig-like domains. It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon RI gamma (4). Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (5). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (6-8).

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Citations

1. NKp46 Calibrates Tumoricidal Potential of Type 1 Innate Lymphocytes by Regulating TRAIL Expression
   Authors: G Turchinovi, S Ganter, A Bärenwaldt, D Finke
   J. Immunol., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Bioassay


2. NK cell heparanase controls tumor invasion and immune surveillance
   Authors: EM Putz, AJ Mayfosh, K Kos, DS Barkauskas, K Nakamura, L Town, KJ Goodall, DY Yee, IK Poon, N Baschuk, F Souza-Fons, MD Hulett, MJ Smyth
   J. Clin. Invest., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


3. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
   Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
   J. Immunol, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. IL-2 in the tumor microenvironment is necessary for Wiskott-Aldrich syndrome protein deficient NK cells to respond to tumors in vivo
   Sci Rep, 2016;6(0):30636.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


5. Independent control of natural killer cell responsiveness and homeostasis at steady-state by CD11c+ dendritic cells
   Sci Rep, 2016;6(0):37996.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


6. Transcription factor KLF2 regulates homeostatic NK cell proliferation and survival
   Authors: W Rabacal, SK Pabbisetty, KL Hoek, D Cendron, Y Guo, D Maseda, E Sebzda
   Proc Natl Acad Sci USA, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Frozen


7. Murine liver-resident group 1 innate lymphoid cells regulate optimal priming of anti-viral CD8+ T cells
   J Leukoc Biol, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC OCT-embedded


8. IL-15 Superagonist-Mediated Immunotoxicity: Role of NK Cells and IFN-gamma.
   Authors: Guo Y, Luan L, Rabacal W, Bohannon J, Fensterheim B, Hernandez A, Sherwood E
   J Immunol, 2015;195(5):2353-64.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


9. NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
   Authors: Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D
   J Leukoc Biol, 2015;97(3):583-98.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


10. Antigen dependently activated cluster of differentiation 8-positive T cells cause perforin-mediated neurotoxicity in experimental stroke.
    Authors: Mracsko E, Liesz A, Stojanovic A, Lou W, Osswald M, Zhou W, Karcher S, Winkler F, Martin-Villalba A, Cerwenka A, Veltkamp R
    J Neurosci, 2014;34(50):16784-95.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay

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