抗VEGF抗体(Anti-VEGF, Canine, Mouse-Mono antibody)
掲載日情報：2021/01/28 現在Webページ番号：30848
VEGFに対する抗体(Anti-VEGF, Canine, Mouse-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月18日 11時15分現在]
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Anti-VEGF, Canine, Mouse-Mono(247109) |
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Anti-Canine VEGF MAb (Clone 247109) |
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[在庫・価格 ：2024年05月18日 11時15分現在]
Anti-VEGF, Canine, Mouse-Mono(247109)
文献数： 1
- 商品コード：MAB1603
- メーカー：RSD
- 包装：500μg
- 価格：¥122,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Canine VEGF サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF1603，スタンダードとして#1603-CV-010を用いる。 別名：MVCD1 クローン：247109 Genbank No： 7422 Protein Accession No： NP_001103972 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Canine | 免疫動物 | Mouse | ||||
| 交差性 | Canine | 適用 | ELISA,IC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
M1/M2 Macrophage Activation Marker |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Canine VEGF MAb (Clone 247109)
文献数： 0
- 商品コード：MAB1603-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 別名：MVCD1 クローン：247109 Genbank No： 7422 Protein Accession No： NP_001103972 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Canine | 適用 | ELISA,IC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 |
M1/M2 Macrophage Activation Marker |
||||||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Canine |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant canine VEGF164Pro28-Arg190Accession # NP_001103972 |
| Source | Monoclonal Mouse IgG1 Clone # 247109 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects canine VEGF in ELISAs and Western blots. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) VEGF121 and rhVEGF165, less than 25% cross-reactivity with recombinant rat VEGF164, and no cross-reactivity with rhVEGF-B186, rhVEGF-C, rhVEGF‑D, recombinant mouse (rm) VEGF115, rmVEGF120, rmVEGF165, or recombinant zebrafish VEGF165 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Canine VEGF (Catalog # 1603-CV) |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Canine VEGF Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Canine VEGF Antibody (Catalog #MAB1603 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Canine VEGF164 Biotinylated Antibody (Catalog #BAF1603 ) |
| ELISA Standard | Recombinant Canine VEGF Protein (Catalog #1603-CV ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Immunocytochemistry |
 click image to view larger | VEGF in Canine PBMCs.VEGF was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine VEGF Monoclonal Antibody (Catalog # MAB1603) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: VEGF | Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternately spliced isoforms of VEGF ranging from 206 amino acids (aa) to 121 aa in length are known. Three isoforms, VEGF188, VEGF182, and VEGF164, have been identified in canine. Canine VEGF164 shares 91%, 90%, and 98% aa sequence identity with the rat, mouse, and feline homologs, respectively. Two type I transmembrane receptor tyrosine kinases, VEGF R1 and VEGF R2, that bind VEGF with high affinity, have been identified. Neuropilin-1, a receptor for semaphorin, also binds VEGF and acts as a co-receptor to enhance the affinity between VEGF and VEGF R2. Neuropilin-1 alone can also mediate VEGF-induced endothelial cell migration. VEGF regulates cell proliferation, migration, and survival of endothelial cells. These functions are partially mediated through the induction of nitric oxide, prostacyclin, and metalloproteinases. Together with angiopoietins or other vascular-specific growth factors, VEGF plays a separate but complementary role in angiogenesis and vasculogenesis (1-7). |
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References
| JingJing, L. et al. (2000) Mol. Cell Biochem. 214:23. | |
| Scheidegger, P. et al. (1999) Biol. Chem. 380:1449. | |
| Thurston, G. (2002) J. Anat. 200:575. | |
| Zachary, I. and G. Gliki (2001) Cardiovasc. Res. 49:568. | |
| Chevalier, S. (2002) Mol. Cell Endocrinol. 189:169. | |
| Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853. | |
| Wang, L. et al. (2003) J. Biol. Chem. 278:48848. | |
| Long Name: | Vascular Endothelial Growth Factor |
| Entrez Gene IDs: | 7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish) |
| Alternate Names: | MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor |
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Related Research Areas
| Adipocytokines | ||
| Adult Neurogenesis | ||
| Airway Inflammation and Remodeling | ||
| Alpha Granule Soluble Molecules | ||
| ALS Related Molecules | ||
| Cancer Biomarkers | ||
| Developmental Factors Implicated in Neurodegeneration | ||
| HIF Transcription Factors | ||
| Inflammatory Mediators | ||
| Macrophage Activation Markers | ||
| Myeloid-derived Suppressor Cells (MDSC) | ||
| Preeclampsia | ||
| VEGF Family | ||
| VEGF Family Ligands | ||
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| Immunocytochemistry | |
|---|---|
 | VEGF in Canine PBMCs. VEGF was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine VEGF Monoclonal Antibody (Catalog # MAB1603) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
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Related Product & Information
| Background | VEGF |
|---|---|
| background_content | Background: VEGF Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternately spliced isoforms of VEGF ranging from 206 amino acids (aa) to 121 aa in length are known. Three isoforms, VEGF188, VEGF182, and VEGF164, have been identified in canine. Canine VEGF164 shares 91%, 90%, and 98% aa sequence identity with the rat, mouse, and feline homologs, respectively. Two type I transmembrane receptor tyrosine kinases, VEGF R1 and VEGF R2, that bind VEGF with high affinity, have been identified. Neuropilin-1, a receptor for semaphorin, also binds VEGF and acts as a co-receptor to enhance the affinity between VEGF and VEGF R2. Neuropilin-1 alone can also mediate VEGF-induced endothelial cell migration. VEGF regulates cell proliferation, migration, and survival of endothelial cells. These functions are partially mediated through the induction of nitric oxide, prostacyclin, and metalloproteinases. Together with angiopoietins or other vascular-specific growth factors, VEGF plays a separate but complementary role in angiogenesis and vasculogenesis (1-7). |
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Citations
- A functional comparison of canine and murine bone marrow derived cultured mast cells.
Authors: Lin TY, London CA,
Vet. Immunol. Immunopathol., 2006;114(3):320-34.
Species: Canine
Sample Type: Cell Culture Supernates
Application: ELISA Development
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