抗IL-1β/IL-1F2抗体(Anti-IL-1β/IL-1F2, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：30663
IL-1β/IL-1F2に対する抗体(Anti-IL-1β/IL-1F2, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
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[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-IL-1β/IL-1F2, Goat-Poly, Biotin
文献数： 0
- 商品コード：BAF1796
- メーカー：RSD
- 包装：50μg
- 価格：¥113,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Feline IL-1β/IL-1F2 サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#AF1796，スタンダードとして#1796-FL-010を用いる。 別名：catabolin Genbank No： 3553 Protein Accession No： P41687 |
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| 法規制等 | |||
| 保存条件 | 法規備考 | ||
| 抗原種 | Feline | 免疫動物 | Goat |
| 交差性 | Feline | 適用 | ELISA,IC,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies R&D Systems社 IL-1β関連製品 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Feline |
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| Label | Biotin |
| Immunogen | E. coli-derived recombinant feline IL‑1 beta Ala116-Ser267Accession # P41687 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects feline IL‑1 beta /IL‑1F2 in ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human IL-1 beta and recombinant mouse IL-1 beta is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Feline IL‑1 beta /IL‑1F2 (Catalog # 1796-FL) |
| Immunocytochemistry | 5-15 µg/mL | See below |
| Feline IL-1 beta /IL-1F2 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Feline IL‑1 beta /IL‑1F2 Antibody (Catalog #AF1796 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Feline IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog #BAF1796 ) |
| ELISA Standard | Recombinant Feline IL-1 beta/IL-1F2 Protein (Catalog #1796-FL ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Immunocytochemistry |
![Immunocytochemistry IL-1 beta/IL-1F2 Antibody [Biotin]](http://resources.rndsystems.com/images/datasheets/antibody/IL1_beta_BAF1796_Immunocytochemistry__Immunofluorescence_17427.jpg) click image to view larger | IL‑1 beta /IL‑1F2 in Feline PBMCs. IL‑1 beta /IL‑1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1796) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-1 beta/IL-1F2 | IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in feline. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with the IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1‑4). The feline IL-1 beta cDNA encodes a 267 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature feline IL-1 beta shares 63%‑78% aa sequence identity with canine, cotton rat, equine, human, mouse, porcine, rat, and rhesus IL-1 beta. |
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References
| Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629. | |
| Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229. | |
| Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S. | |
| Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21. | |
| Accession # AAA30814. | |
| Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10. | |
| Long Name: | Interleukin 1 beta |
| Entrez Gene IDs: | 3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig) |
| Alternate Names: | catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta |
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Related Research Areas
| Immunocytochemistry | |
|---|---|
 | IL‑1 beta /IL‑1F2 in Feline PBMCs. IL‑1 beta /IL‑1F2 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-FelineIL‑1 beta /IL‑1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1796) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
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Related Product & Information
| Background | IL-1 beta/IL-1F2 |
|---|---|
| background_content | Background: IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in feline. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with the IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1‑4). The feline IL-1 beta cDNA encodes a 267 aa precursor. A 115 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature feline IL-1 beta shares 63%‑78% aa sequence identity with canine, cotton rat, equine, human, mouse, porcine, rat, and rhesus IL-1 beta. |
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