抗IL-12抗体(Anti-IL-12, Goat-Poly antibody)
掲載日情報：2021/01/28 現在Webページ番号：30609
IL-12に対する抗体(Anti-IL-12, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年05月01日 00時00分現在]
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Anti-IL-12, Goat-Poly <Anti-IL-23 p40> |
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Anti-Feline IL-12/IL-23 p40 Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2025年05月01日 00時00分現在]
Anti-IL-12, Goat-Poly <Anti-IL-23 p40>
文献数： 1
- 商品コード：AF1954
- メーカー：RSD
- 包装：100μg
- 価格：¥103,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Feline IL-12/IL-23 p40 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF1954，スタンダードとして#2117-FL-025を用いる。 別名：IL-12，IL-23 p40 Genbank No： 493741 Protein Accession No： O02744 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | 法規備考 | ||||||
| 抗原種 | Feline | 免疫動物 | Goat | ||||
| 交差性 | Feline | 適用 | ELISA,IC,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Feline IL-12/IL-23 p40 Affinity Purified Polyclonal Ab
文献数： 0
- 商品コード：AF1954-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 Genbank No： 493741 Protein Accession No： O02744 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Feline | 適用 | ELISA,IC,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Feline |
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| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant feline IL‑12/IL‑23 p40Ile23-Ser329 (Glu167Gly)Accession # O02744 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects feline IL‑12/IL‑23 p40 in ELISAs and Western blots. In sandwich immunoassays, approximately 65% cross‑reactivity with recombinant canine IL-12/23 p40 is observed, less than 3% cross-reactivity with recombinant mouse IL-12/23 p40 is observed, and less than 0.2% cross-reactivity with recombinant human IL‑12/23 p40 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Feline IL‑12/IL‑23 p40 (Catalog # 2117-FL) |
| Immunocytochemistry | 10-25 µg/mL | See below |
| Feline IL-12/IL-23 p40 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Feline IL‑12/IL‑23 p40 Antibody (Catalog #AF1954 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Feline IL‑12/IL‑23 p40 Biotinylated Antibody (Catalog #BAF1954 ) |
| ELISA Standard | Recombinant Feline IL-12/IL-23 p40 Protein (Catalog #2117-FL ) | |
| Neutralization | Measured by its ability to neutralize IL‑12-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 25 ng/mL Recombinant Feline IL‑12. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
![Neutralization IL-12/IL-23 p40 Antibody [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/IL-12_p40_AF1954_Block_Neutralize_8806.jpg) click image to view larger | Cell Proliferation Induced by IL‑12 and Neutralization by Feline IL‑12/IL‑23 p40 Antibody.Recombinant Feline IL‑12 (Catalog # 1954‑FL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‑12 (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL‑12/IL‑23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1954). The ND50 is typically 0.5-2.0 µg/mL. |
| Immunocytochemistry |
![Immunocytochemistry IL-12/IL-23 p40 Antibody [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/IL12_p40_AF1954_Immunocytochemistry__Immunofluorescence_19414.jpg) click image to view larger | IL‑12/IL‑23 p40 in Feline PBMCs.IL‑12/IL‑23 p40 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) stimulated with PMA and calcium ionomycin using Goat Anti-Feline IL‑12/IL‑23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1954) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-12/IL-23 p40 | Interleukin 12 (IL-12) and IL-23 are secreted heterodimeric glycoproteins belonging to the IL-12 cytokine family. The two cytokines share a common p40 (40 kDa) subunit, which is disulfide-linked with the p35 (35 kDa) subunit in IL-12, and with the p19 (19 kDa) subunit in IL-23. Feline p40 is synthesized as a 329 amino acid (aa) precursor with a 22 aa signal sequence and a 307 aa mature region. It contains a 90 aa fibronectin type III domain and a 75 aa Ig C2-like region. The expression of p40 is induced by substances such as LPS and CpG that activate antigen-presenting cells. Besides being found as a component of IL-12 or IL-23, free p40 monomers and homodimers are also secreted by cells expressing p40. Feline p40 shares 94%, 85%, 84%, 65%, and 65% aa sequence identity with canine, human, porcine, rat and mouse p40, respectively. Cells known to express p40 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, plasmacytoid dendritic cells, and microglia. From cells that express both the p35 and p40 subunits (dendritic cells, monocytes, and CHO cells), the amount of free p40 secreted is 10‑1000 fold more than the heterodimeric IL-12. The high-affinity IL-12 receptor complex that transduces IL-12 signals is composed of a 100 kDa ligand-binding subunit (IL-12 R beta 1) and a 130 kDa signal transducing subunit (IL-12 R beta 2). Similarly, the high-affinity IL-23 signaling receptor complex is composed of the shared IL-12 R beta 1 and the unique IL-23 R, a novel gp130-like protein. Both the monomeric and the dimeric free p40 can bind to the IL-12 R beta 1 and function as antagonists of IL-12 or IL-23. However, the monomeric p40 binds IL-12 R beta 1 with lower affinity and is less potent as an IL-12 antagonist. Homodimeric mouse p40 has also been shown to have agonistic functions similar to IL-12, inducing nitric oxide expression and NF kappa B activation in mouse primary microglia and peritoneal macrophages. The molecular mechanism for the agonistic effects of homodimeric p40 has not been determined (1‑6). |
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References
| Buttner, M. et al. (1997) Cytokine 10:241. | |
| Park, A.Y. and P. Scott (2001) Scand. J. Immunol. 53:529. | |
| Trinchieri, G. et al. (2003) Immunity 19:641. | |
| Brombacher, F. et al. (2003) Trends Immunol. 24:207. | |
| Lankford, C.S. and D.M. Frucht, 2003, J. Leukoc. Biol. 73:49. | |
| Abdi, K. (2002) Scand. J. Immunol. 5:1. | |
| Long Name: | Interleukin 12/Interleukin 23 p40 |
| Entrez Gene IDs: | 3593 (Human); 16160 (Mouse); 64546 (Rat); 403976 (Canine); 493741 (Feline) |
| Alternate Names: | CLMF2; IL12 p40; IL-12 p40; IL12B; IL-12B; NKSF2 |
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Related Research Areas
| Cytokines and Receptors in Allergy and Asthma | ||
| Cytokines and Receptors in Angiogenesis | ||
| gamma delta T Cells | ||
| IL-12 Family | ||
| Th1 Cells | ||
| Th17 Cells | ||
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| Neutralization | |
|---|---|
 | Cell Proliferation Induced by IL‑12 and Neutralization by Feline IL‑12/IL‑23 p40 Antibody. Recombinant Feline IL‑12 (Catalog # 1954‑FL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‑12 (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-FelineIL‑12/IL‑23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1954). The ND50 is typically 0.5-2.0 µg/mL. |
| Immunocytochemistry | |
 | IL‑12/IL‑23 p40 in Feline PBMCs. IL‑12/IL‑23 p40 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) stimulated with PMA and calcium ionomycin using Goat Anti-Feline IL‑12/IL‑23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1954) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
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Related Product & Information
| Long Name | Interleukin 12/Interleukin 23 p40 |
|---|---|
| Entrez Gene IDs | 3593 (Human); 16160 (Mouse); 64546 (Rat); 403976 (Canine); 493741 (Feline) |
| Background | IL-12/IL-23 p40 |
| background_content | Background: IL-12/IL-23 p40 Interleukin 12 (IL-12) and IL-23 are secreted heterodimeric glycoproteins belonging to the IL-12 cytokine family. The two cytokines share a common p40 (40 kDa) subunit, which is disulfide-linked with the p35 (35 kDa) subunit in IL-12, and with the p19 (19 kDa) subunit in IL-23. Feline p40 is synthesized as a 329 amino acid (aa) precursor with a 22 aa signal sequence and a 307 aa mature region. It contains a 90 aa fibronectin type III domain and a 75 aa Ig C2-like region. The expression of p40 is induced by substances such as LPS and CpG that activate antigen-presenting cells. Besides being found as a component of IL-12 or IL-23, free p40 monomers and homodimers are also secreted by cells expressing p40. Feline p40 shares 94%, 85%, 84%, 65%, and 65% aa sequence identity with canine, human, porcine, rat and mouse p40, respectively. Cells known to express p40 include macrophages, dendritic cells, monocytes, Langerhans cells, neutrophils, keratinocytes, plasmacytoid dendritic cells, and microglia. From cells that express both the p35 and p40 subunits (dendritic cells, monocytes, and CHO cells), the amount of free p40 secreted is 10‑1000 fold more than the heterodimeric IL-12. The high-affinity IL-12 receptor complex that transduces IL-12 signals is composed of a 100 kDa ligand-binding subunit (IL-12 R beta 1) and a 130 kDa signal transducing subunit (IL-12 R beta 2). Similarly, the high-affinity IL-23 signaling receptor complex is composed of the shared IL-12 R beta 1 and the unique IL-23 R, a novel gp130-like protein. Both the monomeric and the dimeric free p40 can bind to the IL-12 R beta 1 and function as antagonists of IL-12 or IL-23. However, the monomeric p40 binds IL-12 R beta 1 with lower affinity and is less potent as an IL-12 antagonist. Homodimeric mouse p40 has also been shown to have agonistic functions similar to IL-12, inducing nitric oxide expression and NF kappa B activation in mouse primary microglia and peritoneal macrophages. The molecular mechanism for the agonistic effects of homodimeric p40 has not been determined (1‑6). |
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Citations
- Efficient plant-based production of chicken interleukin-12 yields a strong immunostimulatory cytokine.
Authors: Medrano G, Dolan MC, Stephens NT, McMickle A, Erf G, Radin D, Cramer CL
J. Interferon Cytokine Res., 2010;30(3):143-54.
Species: Chicken
Sample Type: Recombinant Protein
Application: WB
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