抗NKp46抗体(Anti-NKp46, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human NKp46/NCR1Gln22-Asn254Accession # AAH64806
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human NKp46 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse NKp46 (MAR-1) is observed and less than 1% cross-reactivity with recombinant human (rh) NKp30, rhNKp44, and rhNKp80 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human NKp46/NCR1 Fc Chimera (Catalog # 1850-NK)
Immunohistochemistry	3-25 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Immunocytochemistry
	NKp46/NCR1 in NK‑92 human cell line NKp46/NCR1 was detected in immersion fixedNK-92 human natural killer lymphoma cell line using Goat Anti-Human NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1850) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
	NKp46/NCR1 in Human Lymph Node. NKp46/NCR1 was detected in immersion fixed paraffin-embedded sections of human lymph node using Goat Anti-Human NKp46/NCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1850) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Related Product & Information

Background

NKp46/NCR1

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Background: NKp46/NCR1 NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. The CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is up-regulated by IL-2. NKp46 is a type I transmembrane protein with two extracellular Ig-like domains followed by a short stalk region, a transmembrane domain containing a positively charged amino acid residue, and a short cytoplasmic tail. Through its positive charge in the transmembrane domain, NKp46 associates with theITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon R1 gamma, which are able to form disulfide-linked homodimers and heterodimers (3, 8). Studies with neutralizing antibodies indicate that the three NCRs are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (4). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (5-7). Human NKp46 shares 58% and 59% amino acid sequence identity with the mouse and rat proteins, respectively.

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Citations

1. Fc receptor-mediated phagocytosis in tissues as a potent mechanism for preventive and therapeutic HIV vaccine strategies
   Mucosal Immunol, 2016;9(6):1584-1595.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


2. Evidence of innate lymphoid cell redundancy in humans
   Authors: F Vély, V Barlogis, B Vallentin, B Neven, C Piperoglou, M Ebbo, T Perchet, M Petit, N Yessaad, F Touzot, J Bruneau, N Mahlaoui, N Zucchini, C Farnarier, G Michel, D Moshous, S Blanche, A Dujardin, H Spits, JH Distler, A Ramming, C Picard, R Golub, A Fischer, E Vivier
   Nat. Immunol, 2016;17(11):1291-1299.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


3. In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.
   Authors: Berglin L, Bergquist A, Johansson H, Glaumann H, Jorns C, Lunemann S, Wedemeyer H, Ellis E, Bjorkstrom N
   PLoS ONE, 2014;9(8):e105375.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Frozen


4. Up-regulation of a death receptor renders antiviral T cells susceptible to NK cell-mediated deletion.
   Authors: Peppa, Dimitra, Gill, Upkar S, Reynolds, Gary, Easom, Nicholas, Pallett, Laura J, Schurich, Anna, Micco, Lorenzo, Nebbia, Gaia, Singh, Harsimra, Adams, David H, Kennedy, Patrick, Maini, Mala K
   J Exp Med, 2013;210(1):99-114.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. Innate immunity in multiple sclerosis white matter lesions: expression of natural cytotoxicity triggering receptor 1 (NCR1).
   Authors: Durrenberger PF, Ettorre A, Kamel F
   J Neuroinflammation, 2012;9(0):1.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC

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