抗Galectin-1抗体(Anti-Galectin-1, Mouse, Goat-Poly antibody)
掲載日情報：2021/01/28 現在Webページ番号：30212
Galectin-1に対する抗体(Anti-Galectin-1, Mouse, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Summary
- Summary
- Summary
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2026年04月06日 15時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Galectin-1, Mouse, Goat-Poly |
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Anti-Mouse Galectin-1 Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2026年04月06日 15時35分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Galectin-1, Mouse, Goat-Poly
文献数： 22
- 商品コード：AF1245
- メーカー：RSD
- 包装：100μg
- 価格：¥81,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Mouse Galectin-1 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF1245，スタンダードとして#1245-GA-050を用いる。 別名：14 kDa laminin-binding protein Genbank No： 3956 Protein Accession No： P16045 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Mouse | 免疫動物 | Goat | ||||
| 交差性 | Mouse | 適用 | ELISA,FCM,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse Galectin-1 Affinity Purified Polyclonal Ab
文献数： 16
- 商品コード：AF1245-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥28,000
- 在庫：1個
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：14 kDa laminin-binding protein Genbank No： 3956 Protein Accession No： P16045 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Mouse | 適用 | ELISA,FCM,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 免疫染色システム ImmPRESS® Reagent Anti-Goat IgG 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant mouse Galectin-1Ala2-Glu135Accession # P16045 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects mouse Galectin-1 in ELISAs and Western blots. In sandwich ELISAs, less than 5% cross-reactivity with recombinant human (rh) Galectin‑1 and less than 0.5% cross-reactivity with recombinant mouse (rm) Galectin-3, rhGalectin-4, rmGalectin-7, and rhGalectin‑8 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Mouse Galectin‑1 (Catalog # 1245-GA) |
| Immunohistochemistry | 5-15 µg/mL | See below |
| CyTOF-ready | Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. | |
| Intracellular Staining by Flow Cytometry | 2.5 µg/106 cells | See below |
| Mouse Galectin-1 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Mouse Galectin‑1 Antibody (Catalog #AF1245 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse Galectin‑1 Biotinylated Antibody (Catalog #BAF1245 ) |
| ELISA Standard | Recombinant Mouse Galectin-1 Protein (Catalog #1245-GA ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Immunohistochemistry |
 click image to view larger | Galectin‑1 in Mouse Thymus.Galectin‑1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
| Intracellular Staining by Flow Cytometry |
 click image to view larger | Detection of Galectin‑1 in Mouse Splenocytes by Flow Cytometry.Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: Galectin-1 | The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4). Mouse Galectin-1, also known as beta-galactoside-binding lectin L-14-I, lactose-binding lectin 1, S-Lac lectin 1, galaptin and 14 kDa lectin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, lymph nodes, spleen, thymus, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth, proliferation and differentiation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Mouse and human Galectin-1 share about 88% amino acid sequence similarity (1-5). |
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References
| Rabinovich, A. et al. (2002) Trends in Immunol. 23:313. | |
| Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741. | |
| Hughes, R.C. (2001) Biochimie 83:667. | |
| R&D Systems’ Cytokine Bulletin, Summer (2002). | |
| Goldring, K. et al. (2002) J. Cell Science 115:355. | |
| Entrez Gene IDs: | 3956 (Human); 16852 (Mouse) |
| Alternate Names: | 14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1 |
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Related Research Areas
| Acute Lymphoblastic Leukemia | ||||||||||
| Adult Neurogenesis | ||||||||||
| Galectins | ||||||||||
| Lectins | ||||||||||
| Multiple Sclerosis and Myelin Related Molecules | ||||||||||
| Regulatory T Cells (Tregs) | ||||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
 Enlarge | Excellent | WB | Mouse | Anonymous | 08/17/2016 | Image Details |
 Western Blot: Mouse Galectin-1 Antibody [AF1245]. | |||
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Summary
| Application | Western Blot | |||||||||
| Sample Tested | U-87 MG human glioblastoma/astrocytoma cell line | |||||||||
| Species | Mouse | |||||||||
| Other Experimental Details | 1:2000 dilution in TBS-Tween 20 + 5% BSA | |||||||||
 Enlarge | Excellent | WB | Human | Anonymous | 10/26/2015 | Image Details |
 Western Blot: Mouse Galectin‑1 Antibody [AF1245]. |
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Summary
| Application | Western Blot | |||||||||
| Sample Tested | Cell Lysates | |||||||||
| Species | Human | |||||||||
| Other Experimental Details | The antibody is very specific at 1:1000 and also at 1:2500 dilutions. Specificity: Specific Sensitivity: Sensitive Buffer: BSA Dilution: 1:1000 | |||||||||
 Enlarge | Excellent | IHC | Mouse | Anonymous | 10/26/2015 | Image Details |
 Immunohistochemistry: Mouse Galectin‑1 Antibody [AF1245]. |
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Summary
| Application | Immunohistochemistry | |||||||||
| Sample Tested | Colon tissue | |||||||||
| Species | Mouse | |||||||||
| Other Experimental Details | The antibody specifically detects the stromal galectin-1 in normal tissues. Specificity: Specific Sensitivity: Sensitive Buffer: PBS Dilution: 1:1000 | |||||||||
 Enlarge | Excellent | ICC/IF | Human | Anonymous | 10/26/2015 | Image Details |
 Immunocytochemistry/Immunofluorescence: Mouse Galectin‑1 Antibody [AF1245]. |
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Summary
| Application | Immunocytochemistry/Immunofluorescence | |||||||||
| Sample Tested | Human fibroblast | |||||||||
| Species | Human | |||||||||
| Other Experimental Details | These are HFF-2 cells that show the staining for this antibody. Specificity: Specific Sensitivity: Sensitive Buffer: PBS` Dilution: 1:500 | |||||||||
 Enlarge | Excellent | ICC/IF | Human | Anonymous | 10/26/2015 | Image Details |
 Immunocytochemistry/Immunofluorescence: Mouse Galectin‑1 Antibody [AF1245]. |
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Summary
| Application | Immunocytochemistry/Immunofluorescence |
| Sample Tested | Cancer Cells |
| Species | Human |
| Other Experimental Details | Very specific in detection of galectin-1 in cancer cells. Specificity: Specific Sensitivity: Sensitive Buffer: PBS Dilution: 1:1000 |
| Immunohistochemistry | |
|---|---|
 | Galectin‑1 in Mouse Thymus. Galectin‑1 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
| Intracellular Staining by Flow Cytometry | |
 | Detection of Galectin‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Normal Goat IgG Control (Catalog # AB-108-C) or (B) Goat Anti-Mouse Galectin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1245) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. |
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Related Product & Information
| Background | Galectin-1 |
|---|---|
| background_content | Background: Galectin-1 The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4).Mouse Galectin-1, also known as beta-galactoside-binding lectin L-14-I, lactose-binding lectin 1, S-Lac lectin 1, galaptin and 14 kDa lectin, is a monomeric or homodimeric prototype galectin that is expressed in a variety of cells and tissues including muscle, heart, lymph nodes, spleen, thymus, macrophages, B cells, T cells, dendritic cells, and tumor cells. It preferentially binds laminin, fibronectin, 90K/Mac-2BP, CD45, CD43, CD7, CD2, CD3, and ganglioside GM1. Galectin-1 modulates cell growth, proliferation and differentiation, either positively or negatively, depending on the cell type and activation status. It controls cell survival by inducing apoptosis of activated T cells and immature thymocytes. It modulates cytokine secretion by inducing Th2 type cytokines and inhibiting pro-inflammatory cytokine production. Galectin-1 can also modulate cell-cell as well as cell-matrix interactions and depending on the cell type and developmental stage, promote cell attachment or detachment. Galectin-1 has immunosuppressive and anti-inflammatory properties and has been shown to suppress acute and chronic inflammation and autoimmunity. Mouse and human Galectin-1 share about 88% amino acid sequence similarity (1-5). |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
- Silencing of galectin-1 inhibits retinal neovascularization and ameliorates retinal hypoxia in a murine model of oxygen-induced ischemic retinopathy
Authors: N Yang, W Zhang, T He, Y Xing
Exp. Eye Res, 2017;0(0):.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC - Mechanism of Ca(2)(+)-triggered ESCRT assembly and regulation of cell membrane repair.
Authors: Scheffer L, Sreetama S, Sharma N, Medikayala S, Brown K, Defour A, Jaiswal J
Nat Commun, 2014;5(0):5646.
Species: Mouse
Sample Type: Cell Lysates
Application: WB - Natural killer cells eradicate galectin-1-deficient glioma in the absence of adaptive immunity.
Authors: Baker G, Chockley P, Yadav V, Doherty R, Ritt M, Sivaramakrishnan S, Castro M, Lowenstein P
Cancer Res, 2014;74(18):5079-90.
Species: Mouse
Sample Type: Cell Lysates
Application: WB - Identification of galectin-1 as a critical factor in function of mouse mesenchymal stromal cell-mediated tumor promotion.
Authors: Szebeni G, Kriston-Pal E, Blazso P, Katona R, Novak J, Szabo E, Czibula A, Fajka-Boja R, Hegyi B, Uher F, Krenacs L, Joo G, Monostori E
PLoS ONE, 2012;7(7):e41372.
Species: Mouse
Sample Type: Whole Cells
Application: ICC - Impaired spatial and contextual memory formation in galectin-1 deficient mice.
Mol Brain, 2011;4(0):33.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Frozen - Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia.
Authors: Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W
J. Neurochem., 2011;116(2):217-26.
Species: Rat
Sample Type: Tissue Homogenates
Application: WB - Endogenous galectin-1 and acute inflammation: emerging notion of a galectin-9 pro-resolving effect.
Authors: Iqbal AJ, Sampaio AL, Maione F, Greco KV, Niki T, Hirashima M, Perretti M, Cooper D
Am. J. Pathol., 2011;178(3):1201-9.
Species: Mouse
Sample Type: Tissue Homogenates
Application: WB - Expression of galectin-1, a new component of slit diaphragm, is altered in minimal change nephrotic syndrome.
Authors: Shimizu M, Khoshnoodi J, Akimoto Y, Kawakami H, Hirano H, Higashihara E, Hosoyamada M, Sekine Y, Kurayama R, Kurayama H, Joh K, Hirabayashi J, Kasai K, Tryggvason K, Ito N, Yan K
Lab. Invest., 2009;89(2):178-95.
Species: Mouse
Sample Type: Tissue Homogenates
Application: WB - Identification of a lectin causing the degeneration of neuronal processes using engineered embryonic stem cells.
Authors: Plachta N, Annaheim C, Bissiere S, Lin S, Ruegg M, Hoving S, Muller D, Poirier F, Bibel M, Barde YA
Nat. Neurosci., 2007;10(6):712-9.
Species: Mouse
Sample Type: Whole Cells
Application: ICC - Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection.
Authors: Silva-Monteiro E, Reis Lorenzato L, Kenji Nihei O, Junqueira M, Rabinovich GA, Hsu DK, Liu FT, Savino W, Chammas R, Villa-Verde DM
Am. J. Pathol., 2007;170(2):546-56.
Species: Mouse
Sample Type: Whole Cells
Application: ICC
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