抗Mouse IFN-gamma抗体(Anti-Mouse IFN-gamma antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IFN-gamma Accession # P01580
Source	Monoclonal Hamster IgG Clone # H22
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IFN-gamma  in direct ELISAs. Does not cross-react with rat, cotton rat, human, rhesus macaque, porcine, feline, or canine IFN-gamma.

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Applications and Data

	Recommended Concentration	Sample
Immunocytochemistry	8-25 µg/mL	See below
Neutralization	Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Schreiber, R. et al. (1985) J. Immunol. 134:1609; Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 4-20 ng/mL in the presence of 2.5 ng/mL Recombinant Mouse IFN‑ gamma.

Neutralization
	IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma Antibody. Recombinant Mouse IFN‑ gamma (Catalog # 485‑MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IFN‑ gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti‑Mouse IFN‑ gamma Monoclonal Antibody (Catalog # MAB4851). The ND50 is typically 4‑20 ng/mL.
Immunocytochemistry
	IFN‑ gamma in Mouse Splenocytes. IFN‑ gamma was detected in immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA (left panel) or unstimulated (right panel) using Hamster Anti-Mouse IFN‑ gamma Monoclonal Antibody (Catalog # MAB4851) at 15 µg/mL for 3 hours at room temperature. Cells were stained using an anti-hamster IgG secondary antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Background	IFN-gamma
background_content	Background: IFN-gamma Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature mouse IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with rat IFN-gamma and 38%‑44% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

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Citations

1. Galectin-1-Driven Tolerogenic Programs Aggravate Yersinia enterocolitica Infection by Repressing Antibacterial Immunity
   Authors: RC Davicino, SP Méndez-Hue, RJ Eliçabe, JC Stupirski, I Autenrieth, MS Di Genaro, GA Rabinovich
   J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: In Vivo


2. The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
   Authors: El-Behi M, Ciric B, Dai H
   Nat. Immunol., 2011;12(6):568-75.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. Lack of TNFR p55 results in heightened expression of IFN-gamma and IL-17 during the development of reactive arthritis.
   Authors: Elicabe RJ, Cargnelutti E, Serer MI, Stege PW, Valdez SR, Toscano MA, Rabinovich GA, Di Genaro MS
   J. Immunol., 2010;185(7):4485-95.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


4. Anti-Asthma Simplified Herbal Medicine Intervention-induced long-lasting tolerance to allergen exposure in an asthma model is interferon-gamma, but not transforming growth factor-beta dependent.
   Authors: Srivastava K, Zhang T, Yang N, Sampson H, Li XM
   Clin. Exp. Allergy, 2010;40(11):1678-88.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


5. Antigen-specific CD25- Foxp3- IFN-gamma(high) CD4+ T cells restrain the development of experimental allergic encephalomyelitis by suppressing Th17.
   Authors: Wildbaum G, Zohar Y, Karin N
   Am. J. Pathol., 2010;176(6):2764-75.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


6. Bystander central memory but not effector memory CD8+ T cells suppress allograft rejection.
   Authors: Wan N, Dai H, Wang T, Moore Y, Zheng XX, Dai Z
   J. Immunol., 2008;180(1):113-21.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


7. Role of Valpha14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of Valpha14+ NKT cells promotes the breakage of CTL tolerance.
   Authors: Ito H, Ando K, Ishikawa T
   Int. Immunol., 2008;20(7):869-79.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


8. In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation.
   Authors: Seshasayee D, Lee WP, Zhou M, Shu J, Suto E, Zhang J, Diehl L, Austin CD, Meng YG, Tan M, Bullens SL, Seeber S, Fuentes ME, Labrijn AF, Graus YM, Miller LA, Schelegle ES, Hyde DM, Wu LC, Hymowitz SG, Martin F
   J. Clin. Invest., 2007;117(12):3868-3878.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut

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