抗CD11b抗体(Anti-CD11b, Rat-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Con A-activated C57BL/10 splenocytes
Source	Monoclonal Rat IgG2B Clone # M1/70
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse CD11b/Integrin alpha M. Cross-reaction with human CD11b/Integrin alpha M has been reported (1, 2).

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	Mouse splenocytes
Immunohistochemistry	Hata, H., et al. (2004) J. Clin. Invest. 114:582. Iwasaki, A. and Kelsall, B.L. (2002) J. Immunol. 166:4884. Zhang, Y., et al. (2002) J. Immunol. 168:3088.
Immunoprecipitation	Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.
CyTOF-reported	Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry

CD11b/Integrin alpha M in Mouse Splenocytes. CD11b/Integrin alpha M was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB1124) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Background

CD11b/Integrin alpha M

background_content

Background: CD11b/Integrin alpha M The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha  and a beta  subunit. The Integrin  alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin  beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin  alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin  alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

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Citations

1. Chi3l3: a potential key orchestrator of eosinophil recruitment in meningitis induced by Angiostrongylus cantonensis
   Authors: S Wan, X Sun, F Wu, Z Yu, L Wang, D Lin, Z Li, Z Wu, X Sun
   J Neuroinflammation, 2018;15(1):31.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


2. CD24 expression does not affect dopamine neuronal survival in a mouse model of Parkinson's disease
   Authors: SR Stott, S Hayat, T Carnwath, S Garas, JP Sleeman, RA Barker
   PLoS ONE, 2017;12(2):e0171748.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Impact of CD200-Fc on dendritic cells in lupus-prone NZB/WF1 mice
   Sci Rep, 2016;6(0):31874.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


4. Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice
   Authors: Clara Jana-Lui Busch
   Hepatology, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Role of Rip2 in development of tumor-infiltrating MDSCs and bladder cancer metastasis.
   Authors: Zhang, Hanwei, Chin, Arnold I
   PLoS ONE, 2014;9(4):e94793.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


6. Absence of T and B lymphocytes modulates dystrophic features in dysferlin deficient animal model.
   Authors: Farini A, Sitzia C, Navarro C, D'Antona G, Belicchi M, Parolini D, Del Fraro G, Razini P, Bottinelli R, Meregalli M, Torrente Y
   Exp. Cell Res., 2012;318(10):1160-74.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


7. Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis.
   J Neuroinflammation, 2012;9(0):193.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


8. Inhibition of experimental autoimmune encephalomyelitis by a novel small molecular weight proinflammatory cytokine suppressing drug.
   Authors: Karpus WJ, Reynolds N, Behanna HA, Van Eldik LJ, Watterson DM
   J. Neuroimmunol., 2008;203(1):73-8.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


9. Sialoadhesin expression in intact degenerating retinas and following transplantation.
   Authors: Sancho-Pelluz J, Wunderlich KA, Rauch U, Romero FJ, van Veen T, Limb GA, Crocker PR, Perez MT
   Invest. Ophthalmol. Vis. Sci., 2008;49(12):5602-10.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


10. LDL-receptor-related protein regulates beta2-integrin-mediated leukocyte adhesion.
    Authors: Spijkers PP, da Costa Martins P, Westein E, Gahmberg CG, Zwaginga JJ, Lenting PJ
    Blood, 2005;105(1):170-7.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP

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