抗Mouse Gr-1抗体(Anti-Mouse Gr-1 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
Source	Monoclonal Rat IgG2B Clone # RB6-8C5
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects Ly-6G/Ly-6C (Gr-1). Weak cross-reactivity with Ly-6C is observed.

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Applications and Data

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/106 cells	Mouse splenocytes or peripheral blood cells
Immunohistochemistry	Nitta, H. et al. (1997) Cell Vision 4:73.
Immunoprecipitation	Conlan, J.W. and R.J. North (1994) J. Exp. Med. 179:259.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry
	Ly-6G/Ly-6C (Gr-1) in Mouse Spleno­cytes. Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed mouse splenocytes using MouseLy-6G/Ly-6C (Gr-1) Monoclonal Anti­body (Catalog # MAB1037) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
	Ly-6G/Ly-6C (Gr-1) in Mouse Spleen.  Ly-6G/Ly-6C (Gr-1) was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1)Monoclonal Antibody (Catalog # MAB1037) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Background	Ly-6G/Ly-6C (Gr-1)
background_content	Background: Ly-6G/Ly-6C (Gr-1) The myeloid differentiation antigen Gr-1 is a member of the Ly-6 family, Ly-6G (1). The RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). In the periphery, this antibody specifically recognized granulocytes (3, 4).

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Citations

1. Vesicular nucleotide transporter mediates ATP release and migration in neutrophils
   Authors: Y Harada, Y Kato, T Miyaji, H Omote, Y Moriyama, M Hiasa
   J. Biol. Chem., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


2. [EXPRESS] Colchicine alleviates acute postoperative pain but delays wound repair in mice: roles of neutrophils and macrophages
   Authors: A Sukeishi, K Isami, H Hiyama, S Imai, K Nagayasu, H Shirakawa, T Nakagawa, S Kaneko
   Mol Pain, 2017;13(0):1744806917743.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-Fr


3. Monocytic and granulocytic myeloid derived suppressor cells differentially regulate spatiotemporal tumour plasticity during metastatic cascade
   Authors: M Ouzounova, E Lee, R Piranliogl, A El Andalou, R Kolhe, MF Demirci, D Marasco, I Asm, A Chadli, KA Hassan, M Thangaraju, G Zhou, AS Arbab, JK Cowell, H Korkaya
   Nat Commun, 2017;8(0):14979.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


4. Diabetic Wounds Exhibit Decreased Ym1 and Arginase Expression with Increased Expression of IL-17 and IL-20
   Authors: Laurie P Shornick
   Adv Wound Care (New Rochelle), 2016;5(11):486-494.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation
   Authors: F Aigner
   Am. J. Transplant., 2016;16(3):808-20.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice
   Authors: Clara Jana-Lui Busch
   Hepatology, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. PTPN22 Is a Critical Regulator of Fc? Receptor-Mediated Neutrophil Activation
   Authors: Sonja Vermeren
   J. Immunol, 2016;197(12):4771-4779.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


8. Discovery of a Metastatic Immune Escape Mechanism Initiated by the Loss of Expression of the Tumour Biomarker Interleukin-33
   Sci Rep, 2016;6(0):30555.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC OCT-embedded


9. Rhadinovirus host entry by co-operative infection.
   Authors: Lawler C, Milho R, May J, Stevenson P
   PLoS Pathog, 2015;11(3):e1004761.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC OCT-embedded


10. The Src-Family Kinases Hck and Fgr Regulate Early Lipopolysaccharide-Induced Myeloid Cell Recruitment into the Lung and Their Ability To Secrete Chemokines.
    Authors: Mazzi P, Caveggion E, Lapinet-Vera J, Lowell C, Berton G
    J Immunol, 2015;195(5):2383-95.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded

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