抗M-CSF抗体(Anti-M-CSF, Human, Goat-Poly, Biotin antibody)
掲載日情報：2021/01/28 現在Webページ番号：29834
M-CSFに対する抗体(Anti-M-CSF, Human, Goat-Poly, Biotin )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月01日 00時01分現在]
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Anti-M-CSF, Human, Goat-Poly, Biotin |
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[在庫・価格 ：2024年05月01日 00時01分現在]
Anti-M-CSF, Human, Goat-Poly, Biotin
文献数： 2
- 商品コード：BAF216
- メーカー：RSD
- 包装：50μg
- 価格：¥119,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Human M-CSF サンドイッチELISAの検出用抗体として利用可能，補足用抗体として#MAB616-500，スタンダードとして#216-MC-005を用いる。 別名：colony stimulating factor 1 (macrophage) Genbank No： 1435 Protein Accession No： NP_757350 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | Human | 免疫動物 | Goat |
| 交差性 | Human | 適用 | ELISA,Western Blot |
| 標識 | Biotin | 性状 | Antigen Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Polyclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 |
抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Biotin |
| Immunogen | E. coli-derived recombinant human M-CSFGlu33-Ser190Accession # NP_757350 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human M-CSF in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse M‑CSF is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human M-CSF (Catalog # 216-MC) |
| Human M-CSF Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human M‑CSF Antibody (Catalog #MAB616 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human M‑CSF Biotinylated Antibody (Catalog #BAF216 ) |
| ELISA Standard | Recombinant Human M-CSF Protein (Catalog #216-MC ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: M-CSF | M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1 - 3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1 - 5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3 - 9). Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific. |
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References
| Pixley, F.J. and E.R. Stanley (2004) Trends Cell Biol. 14:628. | |
| Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39. | |
| Fixe, P. and V. Praloran (1997) Eur. Cytokine Netw. 8:125. | |
| Ryan, G.R. et al. (2001) Blood 98:74. | |
| Makrigiannakis, A. et al. (2006) Trends Endocrinol. Metab. 17:178. | |
| Nandi, S. et al. (2006) Blood 107:786. | |
| Rettenmier, C.W. and M.F. Roussel (1988) Mol. Cell Biol. 8:5026. | |
| Suzu, S. et al. (1992) J. Biol. Chem. 267:16812. | |
| Manos, M.M. (1988) Mol. Cell. Biol. 8:5035. | |
| Koths, K. (1997) Mol. Reprod. Dev. 46:31. | |
| Jang, M-H. et al. (2006) J. Immunol. 177:4055. | |
| Kawasaki, E.S. et al. (1985) Science 230: 291. | |
| Wong, G.G. et al. (1987) Science 235:1504. | |
| Long Name: | Macrophage Colony Stimulating Factor |
| Entrez Gene IDs: | 1435 (Human); 12977 (Mouse) |
| Alternate Names: | colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930 |
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Related Research Areas
| Cancer Biomarkers | ||
| Dendritic Cell Development | ||
| Gametes and Fertilization | ||
| Inflammatory Mediators | ||
| Molecules Secreted by VSMC | ||
| Myeloid-derived Suppressor Cells (MDSC) | ||
| SCF, Flt-3 Ligand and M-CSF | ||
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Related Product & Information
| Background | M-CSF |
|---|---|
| background_content | Background: M-CSF M-CSF, also known as CSF-1, is a four-alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation (1 - 3). M-CSF is also essential for the survival and proliferation of osteoclast progenitors (1, 4). M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis (2, 3). M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta (5). Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells (1 - 5). The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur (3 - 9). Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen (8). Shorter transcripts encode M-CSF that lacks cleavage and PG sites and produces an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer (7). Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor (10, 11). The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively (12, 13). Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific. |
追加しました。
Citations
- A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010;56(0):1320.
Species: Human
Sample Type: Cell Culture Supernates
Application: Luminex Development
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