抗IL-15抗体(Anti-IL-15, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL-15Asn49-Ser162Accession # P48346
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse IL-15 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human IL‑15 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse IL‑15 (Catalog # 447-ML)
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑15-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 0.4-2.4 µg/mL in the presence of 30 ng/mL Recombinant Mouse IL‑15.

Neutralization
	Cell Proliferation Induced by IL‑15 and Neutralization by Mouse IL‑15 Antibody. Recombinant Mouse IL‑15 (Catalog # 447-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑15 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF447). The ND50 is typically 0.4-2.4 µg/mL.
Immunohistochemistry
	IL‑15 in Mouse Intestine. IL‑15 was detected in immersion fixed frozen sections of mouse intestine (Peyer patch) using Goat Anti-Mouse IL‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF447) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes in villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Related Product & Information

Long Name	Interleukin 15
Entrez Gene IDs	3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
Background	IL-15
background_content	Background: IL-15 Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature mouse IL-15 shares 70% and 96% amino acid sequence identity with human and rat IL-15, respectively. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

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Citations

1. TCR-pMHC encounter differentially regulates transcriptomes of(-) tissue-resident CD8 T cells
   Authors: A Yoshikawa, K Bi, DB Keskin, G Zhang, B Reinhold, EL Reinherz
   Eur. J. Immunol., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Frozen


2. ESAT-6-dependent cytosolic pattern recognition drives noncognate tuberculosis control in vivo
   Authors: A Kupz, U Zedler, M Stäber, C Perdomo, A Dorhoi, R Brosch, SH Kaufmann
   J Clin Invest, 2016;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


3. Transcription factor KLF2 regulates homeostatic NK cell proliferation and survival
   Authors: W Rabacal, SK Pabbisetty, KL Hoek, D Cendron, Y Guo, D Maseda, E Sebzda
   Proc Natl Acad Sci USA, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC - Frozen


4. Combination of TACI-IgG and anti-IL-15 treats murine lupus by reducing mature and memory B cells.
   Authors: Ma N, Xiao H, Marrero B, Xing C, Wang X, Zheng M, Han G, Chen G, Hou C, Shen B, Li Y, Wang R, Jiang Z
   Cell Immunol, 2014;289(1):140-4.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


5. Blockade of IL-15 activity inhibits microglial activation through the NFkappaB, p38, and ERK1/2 pathways, reducing cytokine and chemokine release.
   Authors: Gomez-Nicola D, Valle-Argos B, Nieto-Sampedro M
   Glia, 2010;58(3):264-76.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


6. IL-15Ralpha chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentation.
   Authors: Mortier E, Woo T, Advincula R, Gozalo S, Ma A
   J. Exp. Med., 2008;205(5):1213-25.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: ELISA Development


7. A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
   Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J
   Blood, 2007;109(5):2234-42.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: WB

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