抗Endoglin抗体(Anti-Endoglin, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant human Endoglin/CD105Glu26-Gly586Accession # Q5T9B9
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human Endoglin in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant mouse Endoglin and recombinant rat Endoglin is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	U937 human histiocytic lymphoma cell line
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below
Knockout Validated	Endoglin/CD105 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Endoglin/CD105 knockout HeLa cell line.

Western Blot
	Detection of Human Endoglin/CD105 by Western Blot. Western blot shows lysates of human kidney tissue, human lung tissue, human placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endoglin/CD105 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	Endoglin/CD105 in U937 Human Cell Line. Endoglin/CD105 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	Endoglin/CD105 in Human Prostate. Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
Immunohistochemistry
	Endoglin/CD105 in Mouse Kidney. Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Knockout Validated
	Western Blot Shows Human Endoglin/CD105 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Endoglin/CD105 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endoglin/CD105 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Related Product & Information

Background

Endoglin/CD105

background_content

Background: Endoglin/CD105 Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and Betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Human endoglin cDNA encodes 658 amino acids including a 25 amino acid (aa) signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) endoglin (6, 7). The human endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine, and canine endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse endoglin causes lethal vascular and cardiovascular defects, and human endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14).  In preeclampsia of pregnancy, high levels of proteolytically generated soluble endoglin and VEGF R1 (sFlt-1), along with low Placental Growth Factor (PlGF), are pathogenic due to antiangiogenic activity (15).

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Citations

1. Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling
   Authors: Y Jin, L Muhl, M Burmakin, Y Wang, AC Duchez, C Betsholtz, HM Arthur, L Jakobsson
   Nat. Cell Biol., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Hit-and-run programming of therapeutic cytoreagents using mRNA nanocarriers
   Authors: HF Moffett, ME Coon, S Radtke, SB Stephan, L McKnight, A Lambert, BL Stoddard, HP Kiem, MT Stephan
   Nat Commun, 2017;8(1):389.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. A Unique Cellular and Molecular Microenvironment Is Present in Tertiary Lymphoid Organs of Patients with Spontaneous Prostate Cancer Regression
   Authors: ML García-Her, NO Uribe-Urib, R Espinosa-G, WM Kast, SA Khader, J Rangel-Mor
   Front Immunol, 2017;8(0):563.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC-P


4. Novel Rat Model of Repetitive Portal Venous Embolization Mimicking Human Non-Cirrhotic Idiopathic Portal Hypertension
   PLoS ONE, 2016;11(9):e0162144.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


5. Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease
   Sci Rep, 2016;6(0):32382.
   Species: Human
   Sample Type: Whole Cells
   Application: IHC - Not specified


6. Evaluation of Nonperfused Retinal Vessels in Ischemic Retinopathy
   Invest Ophthalmol Vis Sci, 2016;57(11):5031-5037.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Paraffin embedded


7. Umbilical cord mesenchymal stromal cells affected by gestational diabetes mellitus display premature aging and mitochondrial dysfunction.
   Authors: Kim J, Piao Y, Pak Y, Chung D, Han Y, Hong J, Jun E, Shim J, Choi J, Kim C
   Stem Cells Dev, 2015;24(5):575-86.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


8. Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
   Authors: Riordan D, Varma S, West R, Brown P
   PLoS ONE, 2015;10(7):e0128975.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


9. CD105 expression on CD34-negative spindle-shaped stromal cells of primary tumor is an unfavorable prognostic marker in early breast cancer patients.
   Authors: Martinez L, Labovsky V, Calcagno M, Davies K, Garcia Rivello H, Bianchi M, Wernicke A, Fernandez Vallone V, Chasseing N
   PLoS ONE, 2015;10(3):e0121421.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


10. Expression of uPAR in tumor-associated stromal cells is associated with colorectal cancer patient prognosis: a TMA study.
    Authors: Boonstra M, Verbeek F, Mazar A, Prevoo H, Kuppen P, van de Velde C, Vahrmeijer A, Sier C
    BMC Cancer, 2014;14(0):269.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC- Not specified

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