抗MIP-1α抗体(Anti-MIP-1α, Mouse, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse CCL3/MIP-1 alpha Ala24-Ala92Accession # Q5QNW0
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse CCL3/MIP-1 alpha in ELISAs and Western blots. In sandwich ELISAs, less than 0.03% cross-reactivity with recombinant human (rh) CCL3, recombinant mouse (rm) CCL9/10, and rmCCL4 and less than 0.4% cross-reactivity with rhCCL7.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Mouse CCL3/MIP-1 alpha Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	0.2-0.8 µg/mL	Mouse CCL3/MIP‑1 alpha Antibody (Catalog #AF-450-NA )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CCL3/MIP‑1 alpha Biotinylated Antibody (Catalog #BAF450 )
ELISA Standard		Recombinant Mouse CCL3/MIP-1 alpha Protein (Catalog #450-MA )
Neutralization	Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.05-0.2 µg/mL in the presence of 10 ng/mL Recombinant Mouse CCL3/MIP‑1 alpha Isoform LD78a.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Western Blot	click image to view larger	Detection of Mouse CCL3/MIP‑1 alpha by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 μg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL3/MIP‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL3/MIP‑1 alpha at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry	click image to view larger	CCL3/MIP‑1 alpha in Mouse Thymus.CCL3/MIP‑1 alpha was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL3/MIP‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit  (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Neutralization	click image to view larger	Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Mouse CCL3/MIP‑1 alpha Antibody.Recombinant Mouse CCL3/MIP‑1 alpha (Catalog # 450-MA) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL3/ MIP‑1 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL3/MIP‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA). The ND50 is typically 0.05-0.2 µg/mL.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL3/MIP-1 alpha	The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP-1 alpha shares approximately 77% and 70% amino acid identity with human MIP-1 alpha and mouse MIP-1 beta, respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP-1 proteins are members of the beta (C-C) subfamily of chemokines. Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha  has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, MIP-1 beta was reported to be much less active. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.

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References

Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
Entrez Gene IDs:	6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine)
Alternate Names:	C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; Mip1a; MIP-1alpha; MIP1-alpha; Scya3

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Related Research Areas

Show

Alpha Granule Soluble Molecules
CC Chemokines and Receptors
Chemokines and Receptors
Chemokines and Receptors in Allergy and Asthma
Chemokines and Receptors in Angiogenesis
Group 1 ILCs
Infectious Diseases
Inflammatory Mediators
Macrophage Activation Markers
Molecules Secreted by VSMC
Multiple Myeloma
Neuroinflammation
IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury Authors: VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul Sci Rep, 2017;7(0):46514. Species: Mouse Sample Type: Whole Cells Application: Neut
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Very Good		ELISA	Mouse	Anonymous	11/27/2017			Image Details	ELISA: Mouse CCL3/MIP-1 alpha Antibody [AF-450-NA].

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Summary

Application	ELISA
Sample Tested	Serum and Plasma
Species	Mouse
Other Experimental Details	This antibody was paired with BAF450 to build a sandwich ELISA to measure MIP-1a in mouse plasma.

Western Blot
	Detection of Mouse CCL3/MIP‑1 alpha by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 μg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse CCL3/MIP‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL3/MIP‑1 alpha at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	CCL3/MIP‑1 alpha in Mouse Thymus. CCL3/MIP‑1 alpha was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse CCL3/MIP‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog #AF-450-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit  (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Neutralization
	Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutralization by Mouse CCL3/MIP‑1 alpha Antibody. Recombinant MouseCCL3/MIP‑1 alpha (Catalog #450-MA) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL3/MIP‑1 alpha (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL3/MIP‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-450-NA). The ND50 is typically0.05-0.2 µg/mL.

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Related Product & Information

Entrez Gene IDs	6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine)
Background	CCL3/MIP-1 alpha
background_content	Background: CCL3/MIP-1 alpha The macrophage inflammatory proteins 1 alpha and 1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP-1 alpha shares approximately 77% and 70% amino acid identity with human MIP-1 alpha and mouse MIP-1 beta, respectively. MIP‑1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP-1 proteins are members of the beta (C-C) subfamily of chemokines.Both MIP-1 alpha and MIP-1 beta are monocyte chemoattractants in vitro. Additionally, the MIP-1 proteins have been reported to have chemoattractant and adhesive effects on lymphocytes, with MIP-1 alpha and MIP-1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. MIP-1 alpha has also been shown to attract B cells as well as eosinophils. MIP-1 proteins have been reported to have multiple effects on hematopoietic precursor cells and MIP-1 alpha  has been identified as a stem cell inhibitory factor that can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. In the same assays, MIP-1 beta was reported to be much less active. The functional receptor for MIP-1 alpha has been identified as CCR1 and CCR5.

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Citations

1. IFN? Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury
   Authors: VE Thaney, AM O'Neill, MM Hoefer, R Maung, AB Sanchez, M Kaul
   Sci Rep, 2017;7(0):46514.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


2. Hippocampal T cell infiltration promotes neuroinflammation and cognitive decline in a mouse model of tauopathy
   Authors: David Blum
   Brain, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


4. Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines.
   Authors: Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG
   Am. J. Pathol., 2011;179(6):2730-9.
   Species: Mouse
   Sample Type: BALF
   Application: ELISA Development


5. Recruitment and differentiation of conventional dendritic cell precursors in tumors.
   Authors: Diao J, Zhao J, Winter E, Cattral MS
   J. Immunol., 2010;184(3):1261-7.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


6. Comprehensive assessment of chemokine expression profiles by flow cytometry.
   Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
   J. Clin. Invest., 2010;120(3):907-23.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Flow


7. Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS).
   Authors: Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
   J. Biol. Chem., 2009;0(0):.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


8. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
   Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
   J. Immunol., 2009;183(9):5799-806.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


9. CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process.
   Authors: Wu Y, Li YY, Matsushima K, Baba T, Mukaida N
   J. Immunol., 2008;181(9):6384-93.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


10. Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
    Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
    J. Exp. Med., 2008;205(9):2075-84.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo

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