抗TNF-α抗体(Anti-TNF-α, Human, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	Recombinant human TNF-alpha
Source	Monoclonal Mouse IgG1 Clone # 6402
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects cytoplasmic forms of human TNF-alpha.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of TNF‑ alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and Ca2+ ionomycin were stained with Mouse Anti-Human TNF‑ alpha PE‑conjugated Monoclonal Antibody (Catalog # IC210P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Long Name	Tumor Necrosis Factor alpha
Background	TNF-alpha
background_content	Background: TNF-alpha TNF-alpha, designated TNFSF2, is a trimeric glycoprotein active in both membrane bound and secreted forms. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. TNF-alpha binds to TNF RI and TNF RII present on virtually all cell types where it triggers the activation of multiple signal transduction pathways and modulates the expression of a wide variety of genes.

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Citations

1. Serum microRNA181a: Correlates with the intracellular cytokine levels and a potential biomarker for acute graft-versus-host disease
   Authors: Linna Xie
   Cytokine, 2016;85(0):37-44.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Expansion of anti-mesothelin specific CD4+ and CD8+ T cell responses in patients with pancreatic carcinoma.
   Authors: Chen Y, Ayaru L, Mathew S, Morris E, Pereira S, Behboudi S
   PLoS ONE, 2014;9(2):e88133.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
   Authors: Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
   Lab. Invest., 2012;92(7):967-77.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Acetaldehyde stimulates monocyte adhesion in a P-selectin- and TNFalpha-dependent manner.
   Authors: Redmond EM, Morrow D, Kundimi S, Miller-Graziano CL, Cullen JP
   Atherosclerosis, 2009;204(2):372-80.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


5. Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
   Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
   J. Immunol., 2008;180(7):5109-17.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells.
   Authors: Rossol M, Meusch U, Pierer M, Kaltenhauser S, Hantzschel H, Hauschildt S, Wagner U
   J. Immunol., 2007;179(6):4239-48.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Analysis of CD4+ T-Cell responses to a novel alpha-fetoprotein-derived epitope in hepatocellular carcinoma patients.
   Authors: Alisa A, Ives A, Pathan AA, Navarrete CV, Williams R, Bertoletti A, Behboudi S
   Clin. Cancer Res., 2005;11(18):6686-94.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


8. Different domains of Pseudomonas aeruginosa exoenzyme S activate distinct TLRs.
   Authors: Epelman S, Stack D, Bell C, Wong E, Neely GG, Krutzik S, Miyake K, Kubes P, Zbytnuik LD, Ma LL, Xie X, Woods DE, Mody CH
   J. Immunol., 2004;173(3):2031-40.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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