抗Porcine IL-1 beta/IL-1F2抗体(Anti-Porcine IL-1 beta/IL-1F2 antibody)

掲載日情報:2021/01/28 現在Webページ番号:29408

Porcine IL-1 beta/IL-1F2に対する抗体(Anti-Porcine IL-1 beta/IL-1F2 )です。
本製品は研究用です。研究用以外には使用できません。

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Anti-Porcine IL-1 beta/IL-1F2 MAb (Clone 77724)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
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説明文
別名:catabolin
クローン:77724
Genbank No: 3553
Protein Accession No: P26889.
別包装品 別包装品あり
法規制等
保存条件 法規備考
抗原種 免疫動物 Mouse クラス IgG 標識 Unlabeled
交差性 Porcine 適用 ELISA,Neutralising,Western Blot
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
Helper T Cells Marker Antibodies
R&D Systems社 IL-1β関連製品
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド
Anti-Porcine IL-1 beta/IL-1F2 MAb (Clone 77724)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
  • メーカーサイト
  • お問い合わせ
説明文
マッチドペア:Porcine IL-1β/IL-1F2 サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF681,スタンダードとして#681-PI-010を用いる。
別名:catabolin
クローン:77724
Genbank No: 3553
Protein Accession No: P26889.
別包装品 別包装品あり
法規制等
保存条件 法規備考
抗原種 免疫動物 Mouse クラス IgG 標識 Unlabeled
交差性 Porcine 適用 ELISA,Neutralising,Western Blot
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
Helper T Cells Marker Antibodies
R&D Systems社 IL-1β関連製品
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド
Anti-Porcine IL-1 beta/IL-1F2 MAb (Clone 77724)
2~3週間 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 0
  • メーカーサイト
  • お問い合わせ
説明文
※受注発注品。形状:溶液または凍結乾燥
別名:catabolin
クローン:77724
Genbank No: 3553
Protein Accession No: P26889.
別包装品 別包装品あり
法規制等
保存条件 法規備考
抗原種 免疫動物 Mouse クラス IgG 標識 Unlabeled
交差性 Porcine 適用 ELISA,Neutralising,Western Blot
クロナリティ Monoclonal フォーマット 性状 Protein A/G Affinity Purified 吸収処理
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
Helper T Cells Marker Antibodies
R&D Systems社 IL-1β関連製品
使いっきり抗体
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

[在庫・価格 :2025年04月26日 20時55分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Anti-Porcine IL-1 beta/IL-1F2 MAb (Clone 77724)

文献数: 0

説明文 別名:catabolin
クローン:77724
Genbank No: 3553
Protein Accession No: P26889.
別包装品 別包装品あり
法規制等
保存条件 法規備考
抗原種 免疫動物 Mouse
交差性 Porcine 適用 ELISA,Neutralising,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
Helper T Cells Marker Antibodies
R&D Systems社 IL-1β関連製品
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

Anti-Porcine IL-1 beta/IL-1F2 MAb (Clone 77724)

文献数: 0

説明文 マッチドペア:Porcine IL-1β/IL-1F2 サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF681,スタンダードとして#681-PI-010を用いる。
別名:catabolin
クローン:77724
Genbank No: 3553
Protein Accession No: P26889.
別包装品 別包装品あり
法規制等
保存条件 法規備考
抗原種 免疫動物 Mouse
交差性 Porcine 適用 ELISA,Neutralising,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
Helper T Cells Marker Antibodies
R&D Systems社 IL-1β関連製品
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド

Anti-Porcine IL-1 beta/IL-1F2 MAb (Clone 77724)

文献数: 0

説明文 ※受注発注品。形状:溶液または凍結乾燥
別名:catabolin
クローン:77724
Genbank No: 3553
Protein Accession No: P26889.
別包装品 別包装品あり
法規制等
保存条件 法規備考
抗原種 免疫動物 Mouse
交差性 Porcine 適用 ELISA,Neutralising,Western Blot
標識 Unlabeled 性状 Protein A/G Affinity Purified
吸収処理 クラス IgG
クロナリティ Monoclonal フォーマット
掲載カタログ

製品記事 M1/M2 Macrophage Activation Marker
Helper T Cells Marker Antibodies
R&D Systems社 IL-1β関連製品
使いっきり抗体
関連記事 R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド



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Product Details

Species ReactivityPorcine
LabelUnconjugated
ImmunogenE. coli-derived recombinant porcine IL‑1 beta /IL-1F2Ala115-Pro267Accession # P26889.
SourceMonoclonal Mouse IgG1 Clone # 77724
PurificationProtein A or G purified from hybridoma culture supernatant
SpecificityDetects porcine IL-1 beta in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) IL‑1 beta and recombinant mouse (rm) IL‑1 beta is observed and no cross-reactivity with rhIL-1 alpha, rmIL-1 alpha, recombinant porcine IL-1 alpha or recombinant rat IL-1 alpha is observed.


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Applications and Data

 Recommended
Concentration
Sample
Western Blot1 µg/mLRecombinant Porcine IL‑1 beta /IL‑1F2 (Catalog # 681-PI)
Porcine IL-1 beta /IL-1F2 Sandwich ImmunoassayReagent
ELISA Capture (Matched Antibody Pair)2-8 µg/mL Porcine IL‑1 beta /IL‑1F2 Antibody (Catalog #MAB6811 )
ELISA Detection (Matched Antibody Pair)0.1-0.4 µg/mL Porcine IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog #BAF681 )
ELISA Standard  Recombinant Porcine IL-1 beta/IL-1F2 Protein (Catalog #681-PI )
NeutralizationMeasured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 4-15 µg/mL in the presence of 75 ng/mL Recombinant Porcine IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization Neutralization IL-1 beta/IL-1F2 Antibody (77724) [Unconjugated]
click image to view larger
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Porcine
IL‑1 beta /IL‑1F2 Antibody.
Recombinant Porcine
IL‑1 beta /IL‑1F2 (Catalog # 681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine
IL‑1 beta /IL‑1F2 (75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB6811). The ND50 is typically 4-15 µg/mL in the presence of concanavalin A (1.25 µg/mL).
ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.Reconstitution Buffer Available
ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus macaque IL-1 beta.

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References

Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
Huether, M. et al. (1993) Gene 129:285.
Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Long Name:Interleukin 1 beta
Entrez Gene IDs:3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names:catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

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Related Research Areas

Show
Alpha Granule Soluble Molecules
Cancer Biomarkers
Conventional/Classical Dendritic Cells
Cytokines and Receptors in Allergy and Asthma
Cytokines and Receptors in Angiogenesis
IL-1 Family
Infectious Diseases
Inflammatory Mediators
Isolation, Culture, and Identification of Natural Killer Cells
LTi Cells
Macrophage Activation Markers
Multiple Sclerosis and Myelin Related Molecules
Myeloid-derived Suppressor Cells (MDSC)
NCR-ILC3
Neuroinflammation
NOD-like Receptors and the Inflammasome
Regulatory T Cells (Tregs)
T Cell Cytokine Signaling
Th17 Cells
Th9 Cells
  1. Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology.
    Authors: Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M
    Cytometry A, 2006;69(5):391-5.
    Species: Porcine
    Sample Type: Serum
    Application: Luminex Development
ImagesRatingsApplicationsSpeciesReviewed ByDateDetails
Western Blot Porcine IL-1 beta /IL-1F2 Antibody MAB6811 Array MAB6811
Enlarge
Excellent
 WB Porcine Anonymous 04/25/2018 Image Details Western Blot Porcine IL-1 beta /IL-1F2 Antibody MAB6811
Western Blot: Porcine IL-1 beta /IL-1F2 Antibody [MAB6811].

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Summary

Show
Application Western Blot
Sample Tested Cell Lysates
Species Porcine
ELISA Porcine IL-1 beta /IL-1F2 Antibody MAB6811 Array MAB6811
Enlarge
Excellent
 ELISA Porcine Anonymous 11/07/2017 Image Details ELISA Porcine IL-1 beta /IL-1F2 Antibody MAB6811
ELISA: Porcine IL-1 beta /IL-1F2 Antibody [MAB6811].

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Summary

Show
Application ELISA
Sample Tested Serum and Plasma
Species Porcine
Other Experimental Details We used MAB6811 as the capture antibody for Porcine IL-1b in a sandwich ELISA. Biotinylated AF6911 was used as the detection antibody. The antigen standard was 681-PI. We were able to quantify serum and plasma samples.
ELISA Porcine IL-1 beta /IL-1F2 Antibody MAB6811 Array MAB6811
Enlarge
Excellent
 ELISA swine Anonymous 09/07/2017 Image Details ELISA Porcine IL-1 beta /IL-1F2 Antibody MAB6811
ELISA: Porcine IL-1 beta /IL-1F2 Antibody [MAB6811].

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Summary

Application ELISA
Sample Tested EDTA Plasma
Species swine


Neutralization
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by PorcineIL‑1 beta /IL‑1F2 Antibody.
Recombinant PorcineIL‑1 beta /IL‑1F2 (Catalog # 681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant PorcineIL‑1 beta /IL‑1F2 (75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB6811). The ND50 is typically 4-15 µg/mL in the presence of concanavalin A (1.25 µg/mL).


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Related Product & Information

Long NameInterleukin 1 beta
Entrez Gene IDs3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
BackgroundIL-1 beta/IL-1F2
background_contentBackground:
IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus macaque IL-1 beta.


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Citations

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
  1. Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology.
    Authors: Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M
    Cytometry A, 2006;69(5):391-5.
    Species: Porcine
    Sample Type: Serum
    Application: Luminex Development

  2. Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate.
    Authors: Zhang F, Hopwood P, Abrams CC, Downing A, Murray F, Talbot R, Archibald A, Lowden S, Dixon LK
    J. Virol., 2006;80(21):10514-21.
    Species: Porcine
    Sample Type: Cell Lysates
    Application: WB



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