抗Porcine IL-1 beta/IL-1F2抗体(Anti-Porcine IL-1 beta/IL-1F2 antibody)
掲載日情報:2021/01/28 現在Webページ番号:29408
Porcine IL-1 beta/IL-1F2に対する抗体(Anti-Porcine IL-1 beta/IL-1F2 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Summary
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 :2025年04月26日 20時55分現在]
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Anti-Porcine IL-1 beta/IL-1F2 MAb (Clone 77724) |
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[在庫・価格 :2025年04月26日 20時55分現在]
Anti-Porcine IL-1 beta/IL-1F2 MAb (Clone 77724)
文献数: 0
- 商品コード:MAB6811-100
- メーカー:RSD
- 包装:100μg
- 価格:¥101,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 別名:catabolin クローン:77724 Genbank No: 3553 Protein Accession No: P26889. |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | 法規備考 | ||||||
抗原種 | 免疫動物 | Mouse | |||||
交差性 | Porcine | 適用 | ELISA,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies R&D Systems社 IL-1β関連製品 |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Porcine IL-1 beta/IL-1F2 MAb (Clone 77724)
文献数: 0
- 商品コード:MAB6811-500
- メーカー:RSD
- 包装:500μg
- 価格:¥232,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | マッチドペア:Porcine IL-1β/IL-1F2 サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF681,スタンダードとして#681-PI-010を用いる。 別名:catabolin クローン:77724 Genbank No: 3553 Protein Accession No: P26889. |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | 法規備考 | ||||||
抗原種 | 免疫動物 | Mouse | |||||
交差性 | Porcine | 適用 | ELISA,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies R&D Systems社 IL-1β関連製品 |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Porcine IL-1 beta/IL-1F2 MAb (Clone 77724)
文献数: 0
- 商品コード:MAB6811-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | ※受注発注品。形状:溶液または凍結乾燥 別名:catabolin クローン:77724 Genbank No: 3553 Protein Accession No: P26889. |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | 法規備考 | ||||||
抗原種 | 免疫動物 | Mouse | |||||
交差性 | Porcine | 適用 | ELISA,Neutralising,Western Blot | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies R&D Systems社 IL-1β関連製品 使いっきり抗体 |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Porcine |
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Label | Unconjugated |
Immunogen | E. coli-derived recombinant porcine IL‑1 beta /IL-1F2Ala115-Pro267Accession # P26889. |
Source | Monoclonal Mouse IgG1 Clone # 77724 |
Purification | Protein A or G purified from hybridoma culture supernatant |
Specificity | Detects porcine IL-1 beta in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) IL‑1 beta and recombinant mouse (rm) IL‑1 beta is observed and no cross-reactivity with rhIL-1 alpha, rmIL-1 alpha, recombinant porcine IL-1 alpha or recombinant rat IL-1 alpha is observed. |
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Applications and Data
Recommended Concentration | Sample | |
Western Blot | 1 µg/mL | Recombinant Porcine IL‑1 beta /IL‑1F2 (Catalog # 681-PI) |
Porcine IL-1 beta /IL-1F2 Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Porcine IL‑1 beta /IL‑1F2 Antibody (Catalog #MAB6811 ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Porcine IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog #BAF681 ) |
ELISA Standard | Recombinant Porcine IL-1 beta/IL-1F2 Protein (Catalog #681-PI ) | |
Neutralization | Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 4-15 µg/mL in the presence of 75 ng/mL Recombinant Porcine IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A. | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. |
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Data Examples
Neutralization |
![]() click image to view larger | Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Porcine IL‑1 beta /IL‑1F2 Antibody.Recombinant Porcine IL‑1 beta /IL‑1F2 (Catalog # 681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑1 beta /IL‑1F2 (75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB6811). The ND50 is typically 4-15 µg/mL in the presence of concanavalin A (1.25 µg/mL). |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: IL-1 beta/IL-1F2 | IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus macaque IL-1 beta. |
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References
Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629. | |
Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229. | |
Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S. | |
Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21. | |
Huether, M. et al. (1993) Gene 129:285. | |
Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10. | |
Long Name: | Interleukin 1 beta |
Entrez Gene IDs: | 3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig) |
Alternate Names: | catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta |
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Related Research Areas
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Summary
Application | Western Blot | |||||||||
Sample Tested | Cell Lysates | |||||||||
Species | Porcine | |||||||||
![]() Enlarge | Excellent | ELISA | Porcine | Anonymous | 11/07/2017 | Image Details |
![]() ELISA: Porcine IL-1 beta /IL-1F2 Antibody [MAB6811]. |
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Summary
Application | ELISA | |||||||||
Sample Tested | Serum and Plasma | |||||||||
Species | Porcine | |||||||||
Other Experimental Details | We used MAB6811 as the capture antibody for Porcine IL-1b in a sandwich ELISA. Biotinylated AF6911 was used as the detection antibody. The antigen standard was 681-PI. We were able to quantify serum and plasma samples. | |||||||||
![]() Enlarge | Excellent | ELISA | swine | Anonymous | 09/07/2017 | Image Details |
![]() ELISA: Porcine IL-1 beta /IL-1F2 Antibody [MAB6811]. |
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Summary
Application | ELISA |
Sample Tested | EDTA Plasma |
Species | swine |
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Related Product & Information
Long Name | Interleukin 1 beta |
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Entrez Gene IDs | 3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig) |
Background | IL-1 beta/IL-1F2 |
background_content | Background: IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus macaque IL-1 beta. |
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Citations
- Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology.
Authors: Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M
Cytometry A, 2006;69(5):391-5.
Species: Porcine
Sample Type: Serum
Application: Luminex Development - Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate.
Authors: Zhang F, Hopwood P, Abrams CC, Downing A, Murray F, Talbot R, Archibald A, Lowden S, Dixon LK
J. Virol., 2006;80(21):10514-21.
Species: Porcine
Sample Type: Cell Lysates
Application: WB
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