抗TIM-1抗体(Anti-TIM-1, Goat-Poly antibody)
掲載日情報：2021/01/28 現在Webページ番号：28997
TIM-1に対する抗体(Anti-TIM-1, Goat-Poly )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月03日 00時01分現在]
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Anti-TIM-1, Goat-Poly |
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Anti-Human TIM-1/KIM-1/HAVCR Affinity Purified Polyclonal Ab |
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[在庫・価格 ：2024年05月03日 00時01分現在]
Anti-TIM-1, Goat-Poly
文献数： 8
- 商品コード：AF1750
- メーカー：RSD
- 包装：100μg
- 価格：¥106,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Human TIM-1/KIM-1/HAVCR サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF1750，スタンダードとして#1750-TM-050を用いる。 別名：CD365 Genbank No： 26762 Protein Accession No： Q96D42 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Human | 免疫動物 | Goat | ||||
| 交差性 | Human | 適用 | ELISA,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 |
免疫染色システム ImmPRESS® Reagent Anti-Goat IgG |
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| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human TIM-1/KIM-1/HAVCR Affinity Purified Polyclonal Ab
文献数： 1
- 商品コード：AF1750-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 別名：CD365 Genbank No： 26762 Protein Accession No： Q96D42 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Goat | |||||
| 交差性 | Human | 適用 | ELISA,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Antigen Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Polyclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 |
免疫染色システム ImmPRESS® Reagent Anti-Goat IgG |
||||||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human TIM‑1/KIM-1/HAVCR (R&D Systems, Catalog # 1750-TM)Ser21-Thr288Accession # Q96D42 |
| Source | Polyclonal Goat IgG |
| Purification | Antigen Affinity-purified |
| Specificity | Detects human TIM‑1/KIM-1/HAVCR in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant mouse (rm) TIM-1, recombinant rat TIM-1, and recombinant human (rh) TIM-4 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 0.1 µg/mL | Recombinant Human TIM‑1/KIM‑1/HAVCR (Catalog # 1750-TM) |
| Immunohistochemistry | 1-15 µg/mL | See below |
| Human TIM-1/KIM-1/HAVCR Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 0.2-0.8 µg/mL | Human TIM‑1/KIM‑1/HAVCR Antibody (Catalog #AF1750 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human TIM‑1/KIM‑1/HAVCR Biotinylated Antibody (Catalog #BAF1750 ) |
| ELISA Standard | Recombinant Human TIM-1/KIM-1/HAVCR Protein, CF (Catalog #1750-TM ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Immunohistochemistry |
![Immunohistochemistry TIM-1/KIM-1/HAVCR Antibody [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/TIM1_AF1750_Immunohistochemistry_20209.jpg) click image to view larger | TIM‑1/KIM‑1/HAVCR in Human Kidney Cancer Tissue. TIM‑1/KIM‑1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1750) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
| Reconstitution | Reconstitute at 0.2 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: TIM-1/KIM-1/HAVCR | TIM-1 (T cell-immunoglobulin-mucin; also known as KIM-1 and HAVCR) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-3). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain (4-6). The ECD contains oneV-type Ig-like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation. The TIM-1 gene is highly polymorphic and undergoes alternate splicing (1). For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A (7, 8). There are two cytoplasmic alternate splice forms of TIM‑1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in 1a (6). TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85-90 kDa soluble molecule (6). The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (4, 9). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). |
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References
| Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471. | |
| Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. | |
| Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681. | |
| Feigelstock, D. et al. (1998) J. Virol. 72:6621. | |
| Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135. | |
| Bailly, V. et al. (2002) J. Biol. Chem. 277:39739. | |
| Umetsu, D.T. et al. (2005) J. Pediatr. Gastroenterol. Nutr. 40:S43. | |
| Gao, P-S. et al. (2005) J. Allergy Clin. Immunol. 115:982. | |
| Zhu, C. et al. (2005) Nat. Immunol. 6:1245. | |
| Meyers, J.H. et al. (2005) Nat. Immunol. 6:455. | |
| Long Name: | T Cell Immunoglobulin Mucin-1 |
| Entrez Gene IDs: | 26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey) |
| Alternate Names: | CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1 |
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Related Research Areas
| B Cell Markers | ||||||||
| Co-stimulatory and Co-inhibitory Molecules | ||||||||
| Infectious Diseases | ||||||||
| Renal Markers | ||||||||
| Special Glycoproteins | ||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||
|---|---|---|---|---|---|---|---|---|
| Very Good | Detection Assay | Human | Anonymous | 12/15/2017 | ||||
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Summary
| Application | Detection Assay |
| Sample Tested | Recombinant protein |
| Species | Human |
| Immunohistochemistry | |
|---|---|
 | TIM‑1/KIM‑1/HAVCR in Human Kidney Cancer Tissue. TIM‑1/KIM‑1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human TIM‑1/KIM‑1/HAVCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1750) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
追加しました。
Related Product & Information
| Background | TIM-1/KIM-1/HAVCR |
|---|---|
| background_content | Background: TIM-1/KIM-1/HAVCR TIM-1 (T cell-immunoglobulin-mucin; also known as KIM-1 and HAVCR) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-3). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain (4-6). The ECD contains oneV-type Ig-like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation. The TIM-1 gene is highly polymorphic and undergoes alternate splicing (1). For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A (7, 8). There are two cytoplasmic alternate splice forms of TIM‑1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in 1a (6). TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85-90 kDa soluble molecule (6). The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (4, 9). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). |
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Citations
- Human Sertoli cells support high levels of Zika virus replication and persistence
Authors: A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman
Sci Rep, 2018;8(1):5477.
Species: Human
Sample Type: Whole Cells
Application: Neut - Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells
Nat Protoc, 2017;12(1):195-207.
Species: Human
Sample Type: Whole Tissue
Application: IHC Not Specified - Urinary kidney injury molecule?1 as an early diagnostic biomarker of obstructive acute kidney injury and development of a rapid detection method
Authors: Y Jin, X Shao, B Sun, C Miao, Z Li, Y Shi
Mol Med Rep, 2017;0(0):.
Species: Rat
Sample Type: Urine
Application: Immunochromatographic Assay - Nanoparticle Detection of Urinary Markers for Point-of-Care Diagnosis of Kidney Injury.
Authors: Chung H, Pellegrini K, Chung J, Wanigasuriya K, Jayawardene I, Lee K, Lee H, Vaidya V, Weissleder R
PLoS ONE, 2015;10(7):e0133417.
Species: Human
Sample Type: Urine
Application: ELISA Development - Characterizing functional domains for TIM-mediated enveloped virus entry.
Authors: Moller-Tank S, Albritton L, Rennert P, Maury W
J Virol, 2014;88(12):6702-13.
Species: Human
Sample Type: Whole Cells
Application: Flow
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