抗CD117/c-kit抗体(Anti-CD117/c-kit, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kitGln25-Thr519 (Ala207Glu)Accession # P05532
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human and mouse CD117/c-kit in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD117 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Simple Western	5 µg/mL	See below
Flow Cytometry	2.5 µg/106 cells	Lineage depleted mouse bone marrow cells
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunohistochemistry
	CD117/c-kit in Mouse Embryo. CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Western Blot
	Detection of Human and Mouse CD117/c-kit by Western Blot. Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western
	Detection of Human and Mouse CD117/c-kit by Simple WesternTM. Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Long Name	Stem Cell Factor Receptor
Background	CD117/c-kit
background_content	Background: CD117/c-kit Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells.

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Citations

1. Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis
   Authors: Y Kawai, A Oda, Y Kanai, R Goitsuka
   PLoS ONE, 2018;13(1):e0190702.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


2. Multicolor quantitative confocal imaging cytometry
   Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
   Nat. Methods, 2018;15(1):39-46.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


3. Esrp1 is a marker of mouse fetal germ cells and differentially expressed during spermatogenesis
   Authors: S Saeidi, F Shapouri, RU de Iongh, F Casagranda, JM Sutherland, PS Western, EA McLaughlin, M Familari, GR Hime
   PLoS ONE, 2018;13(1):e0190925.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


4. Cardiac Nestin+ Cells Derived from Early Stage of Dilated Cardiomyopathy Enhanced the Survival of the Doxorubicin-Injured Cardiac Muscle HL-1 Cells
   Authors: D Xie, Y Liao, B Wu, Y Chen, W Lin, D Lu, S Gao, S Zhu, C Peng, MH Jiang
   Int Heart J, 2018;59(1):180-189.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


5. A transit-amplifying population underpins the efficient regenerative capacity of the testis
   Authors: C Carrieri, S Comazzetto, A Grover, M Morgan, A Buness, C Nerlov, D O'Carroll
   J. Exp. Med., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


6. Live cell screening platform identifies PPAR? as a regulator of cardiomyocyte proliferation and cardiac repair
   Authors: A Magadum, Y Ding, L He, T Kim, MD Vasudevara, Q Long, K Yang, N Wickramasi, HV Renikunta, N Dubois, G Weidinger, Q Yang, FB Engel
   Cell Res., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


7. Concurrent Isolation of Three Distinct Cardiac Stem Cell Populations from a Single Human Heart Biopsy
   Authors: MM Monsanto, KS White, T Kim, BJ Wang, K Fisher, K Ilves, FG Khalafalla, A Casillas, K Broughton, S Mohsin, WP Dembitsky, MA Sussman
   Circ. Res., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


8. Fabrication of Synthetic Mesenchymal Stem Cells for the Treatment of Acute Myocardial Infarction in Mice
   Authors: L Luo, J Tang, K Nishi, C Yan, PU Dinh, J Cores, T Kudo, J Zhang, TS Li, K Cheng
   Circ. Res, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


9. Paracrine GABA and insulin regulate pancreatic alpha cell proliferation in a mouse model of type 1 diabetes
   Authors: AL Feng, YY Xiang, L Gui, G Kaltsidis, Q Feng, WY Lu
   Diabetologia, 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


10. Estrogen induces c-Kit and an aggressive phenotype in a model of invasive lobular breast cancer
    Authors: JC Harrell, TM Shroka, BM Jacobsen
    Oncogenesis, 2017;6(11):2.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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