抗Endoglin抗体(Anti-Endoglin, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Endoglin/CD105Glu27-Gly581Accession # Q8K100
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects mouse Endoglin in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant rat Endoglin is observed and less than 5% cross-reactivity with recombinant human Endoglin is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	5 µg/mL	See below
Flow Cytometry	2.5 µg/106 cells	MS-1 mouse cell line
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below

Western Blot
	Detection of Mouse Endoglin/CD105 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line and MS‑1 mouse pancreatic islet endothelial cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Endoglin/CD105 at approximately 90-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	Endoglin/CD105 in Rat Mesenchymal Stem Cells. Endoglin/CD105 was detected in immersion fixed rat mesenchymal stem cells using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunocytochemistry
	Endoglin/CD105 in MS-1 Mouse Cell Line. Endoglin/CD105 was detected in immersion fixed MS-1 mouse pancreatic islet endothelial cell line using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
	Endoglin/CD105 in Mouse Embryo. Endoglin/CD105 was detected in immersion fixed frozen sections of mouse embryo (E13-15) using Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western
	Detection of Mouse Endoglin/CD105 by Simple WesternTM. Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Endoglin/CD105 at approximately 121 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1320) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

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Related Product & Information

Background

Endoglin/CD105

background_content

Background: Endoglin/CD105 Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

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Citations

1. Multicolor quantitative confocal imaging cytometry
   Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
   Nat. Methods, 2018;15(1):39-46.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


2. Blockade of myeloid-derived suppressor cell expansion with all-trans retinoic acid increases the efficacy of anti-angiogenic therapy
   Authors: R Bauer, F Udonta, M Wroblewski, I Ben-Batall, IM Santos, F Taverna, M Kuhlencord, V Gensch, S Päsler, S Vinckier, JM Brandner, K Pantel, C Bokemeyer, T Vogl, J Roth, P Carmeliet, S Loges
   Cancer Res., 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC-P


3. Pharmacologic or Genetic Targeting of Glutamine Synthetase Skews Macrophages toward an M1-like Phenotype and Inhibits Tumor Metastasis
   Authors: EM Palmieri, A Menga, R Martín-Pér, A Quinto, C Riera-Domi, G De Tullio, DC Hooper, WH Lamers, B Ghesquière, DW McVicar, A Guarini, M Mazzone, A Castegna
   Cell Rep, 2017;20(7):1654-1666.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


4. DNA sequences within glioma-derived extracellular vesicles can cross the intact blood-brain barrier and be detected in peripheral blood of patients
   Authors: N García-Rom, J Carrión-Na, S Esteban-Ru, E Lázaro-Ibá, M Peris-Celd, MM Alonso, J Guzmán-De-, C Fernández-, AO de Mendivi, S García-Duq, C Escobedo-L, R Prat-Acín, C Belda-Inie, A Ayuso-Saci
   Oncotarget, 2017;8(1):1416-1428.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


5. Mast cells decrease efficacy of anti-angiogenic therapy by secreting matrix-degrading granzyme B
   Authors: M Wroblewski, R Bauer, M Cubas Córd, F Udonta, I Ben-Batall, K Legler, C Hauser, J Egberts, M Janning, J Velthaus, C Schulze, K Pantel, C Bokemeyer, S Loges
   Nat Commun, 2017;8(1):269.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


6. PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia
   Nat Commun, 2016;7(0):13650.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC


7. Gene Electrotransfer of Plasmid with Tissue Specific Promoter Encoding shRNA against Endoglin Exerts Antitumor Efficacy against Murine TS/A Tumors by Vascular Targeted Effects.
   Authors: Stimac M, Dolinsek T, Lampreht U, Cemazar M, Sersa G
   PLoS ONE, 2015;10(4):e0124913.
   Species: Mouse
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. LF-15 & T7, synthetic peptides derived from tumstatin, attenuate aspects of airway remodelling in a murine model of chronic OVA-induced allergic airway disease.
   Authors: Grafton K, Moir L, Black J, Hansbro N, Hansbro P, Burgess J, Oliver B
   PLoS ONE, 2014;9(1):e85655.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Not specified


9. Multiple delivery of siRNA against endoglin into murine mammary adenocarcinoma prevents angiogenesis and delays tumor growth.
   Authors: Dolinsek T, Markelc B, Sersa G, Coer A, Stimac M, Lavrencak J, Brozic A, Kranjc S, Cemazar M
   PLoS ONE, 2013;8(3):e58723.
   Species: Human
   Sample Type: Whole Cells
   Application: IF


10. Focal adhesion kinase regulates the localization and retention of pro-B cells in bone marrow microenvironments.
    Authors: Park S, Wolfram P, Canty K, Harley B, Nombela-Arrieta C, Pivarnik G, Manis J, Beggs H, Silberstein L
    J Immunol, 2013;190(3):1094-102.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded

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