抗VEGF抗体(Anti-VEGF, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF165Ala27-Arg191Accession # NP_001165097.1
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human VEGF in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant canine VEGF is observed, and less then 20% cross-reactivity with recombinant mouse VEGF165 and recombinant rat VEGF164 is observed, and less than 5% cross-reactivity with recombinant zebrafish VEGF is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	5-10 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize VEGF165-induced proliferation in HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The Neutralization Dose (ND50) is typically 0.02‑0.12 µg/mL in the presence of 10 ng/mL Recombinant Human VEGF165.

Western Blot
	Detection of Human VEGF by Western Blot. Western blot shows lysates of human lung tissue, human kidney tissue, and human liver tissue. PVDF membrane was probed with 5-10 µg/mL of Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VEGF at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	VEGF in HUVEC Cells. VEGF was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) using Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Neutralization
	Cell Proliferation Induced by VEGF165 and Neutralization by Human VEGF Antibody. Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF165 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑293‑NA). The ND50 is typically 0.02-0.12 µg/mL.

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Related Product & Information

Long Name	Vascular Endothelial Growth Factor
Entrez Gene IDs	7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Background	VEGF
background_content	Background: VEGF VEGF is a soluble protein secreted by a wide variety of cell types. It binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (Flk-1). VEGF stimulates vascular endothelial cell proliferation and is a potent inducer of angiogenesis. Several VEGF isoforms occur resulting from alternative mRNA splicing.

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Citations

1. Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages
   Authors: S Donzelli, E Milano, M Pruszko, A Sacconi, S Masciarell, I Iosue, E Melucci, E Gallo, I Terrenato, M Mottolese, M Zylicz, A Zylicz, F Fazi, G Blandino, G Fontemaggi
   Breast Cancer Res., 2018;20(1):59.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies
   Authors: R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson
   Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
   Application: Affinity Assay


3. A randomized, single-blind, Phase I trial (INVICTAN-1) assessing the bioequivalence and safety of BI 695502, a bevacizumab biosimilar candidate, in healthy subjects
   Authors: W Hettema, C Wynne, B Lang, M Altendorfe, N Czeloth, R Lohmann, S Athalye, D Schliephak
   Expert Opin Investig Drugs, 2017;0(0):.
   Species: Human
   Sample Type: Protein
   Application: ELISA detection


4. Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease
   Authors: J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard
   PLoS ONE, 2017;12(9):e0184039.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


5. CD147 promotes the proliferation, invasiveness, migration and angiogenesis of human lung carcinoma cells
   Authors: S Yang, F Qi, C Tang, H Wang, H Qin, X Li, J Li, W Wang, C Zhao, H Gao
   Oncol Lett, 2017;13(2):898-904.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression
   Authors: WW Xu, B Li, XY Guan, SK Chung, Y Wang, YL Yip, SY Law, KT Chan, NP Lee, KW Chan, LY Xu, EM Li, SW Tsao, QY He, AL Cheung
   Nat Commun, 2017;8(0):14399.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently
   Authors: L Zheng, C Zhao, Y Du, X Lin, Y Jiang, C Lee, G Tian, J Mi, X Li, Q Chen, Z Ye, L Huang, S Wang, X Ren, L Xing, W Chen, D Huang, Z Gao, S Zhang, W Lu, Z Tang, B Wang, R Ju, X Li
   Oncotarget, 2016;7(47):77902-77915.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling.
   Authors: Shvartsman, Dmitry, Storrie-White, Hannah, Lee, Kangwon, Kearney, Cathal, Brudno, Yevgeny, Ho, Nhi, Cezar, Christin, McCann, Corey, Anderson, Erin, Koullias, John, Tapia, Juan Car, Vandenburgh, Herman, Lichtman, Jeff W, Mooney, David J
   Mol Ther, 2014;22(7):1243-53.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


9. Endothelial cell adhesion to soluble vascular endothelial growth factor receptor-1 triggers a cell dynamic and angiogenic phenotype.
   Authors: Orecchia A, Mettouchi A, Uva P, Simon G, Arcelli D, Avitabile S, Ragone G, Meneguzzi G, Pfenninger K, Zambruno G, Failla C
   FASEB J, 2014;28(2):692-704.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. Detection of VEGF-A(xxx)b isoforms in human tissues.
    Authors: Bates, David O, Mavrou, Athina, Qiu, Yan, Carter, James G, Hamdollah-Zadeh, Maryam, Barratt, Shaney, Gammons, Melissa, Millar, Ann B, Salmon, Andrew H, Oltean, Sebastia, Harper, Steven J
    PLoS ONE, 2013;8(7):e68399.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP

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