抗IFN-γ抗体(Anti-IFN-γ, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF)Met1-Gln144Accession # Q14609
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant rat IFN-gamma and recombinant mouse IFN-gamma is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IFN-gamma (Catalog # 285-IF)
Immunocytochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 5 ng/mL Recombinant Human IFN‑ gamma.

Neutralization
	IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma Antibody. Recombinant Human IFN‑ gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN‑ gamma (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA). The ND50 is typically 0.1‑0.3 µg/mL.
Immunocytochemistry
	IFN‑ gamma in Human PBMCs. IFN‑ gamma was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PMA and ionomycin using 10 µg/mL Goat Anti-Human IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑285‑NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Background	IFN-gamma
background_content	Background: IFN-gamma Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

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Citations

1. The Therapeutic Effect of Pamidronate on Lethal Avian Influenza A H7N9 Virus Infected Humanized Mice.
   Authors: Zheng J, Wu W, Liu Y, Xiang Z, Liu M, Chan K, Lau S, Lam K, To K, Chan J, Li L, Chen H, Lau Y, Yuen K, Tu W
   PLoS ONE, 2015;10(8):e0135999.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. Immunoproteasome expression is induced in mesial temporal lobe epilepsy.
   Authors: Mishto M, Ligorio C, Bellavista E, Martucci M, Santoro A, Giulioni M, Marucci G, Franceschi C
   Biochem. Biophys. Res. Commun., 2011;408(1):65-70.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


3. Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma.
   Authors: Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C
   PLoS Pathog., 2011;7(4):e1002016.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
   Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
   Blood, 2010;116(12):2061-9.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Not specified


5. IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
   Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK
   Immunol. Lett., 2010;127(2):150-6.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Evolving immunosuppressive microenvironment during human cervical carcinogenesis.
   Authors: Kobayashi A, Weinberg V, Darragh T, Smith-McCune K
   Mucosal Immunol, 2008;1(5):412-20.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


7. Upregulated IL-23 and IL-17 in Behcet patients with active uveitis.
   Authors: Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A
   Invest. Ophthalmol. Vis. Sci., 2008;49(7):3058-64.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
   Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
   Eur. J. Immunol., 2008;38(4):1012-23.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma.
   Authors: Hessian PA, Highton J, Kean A, Sun CK, Chin M
   Arthritis Rheum., 2003;48(2):334-8.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


10. Natural killer cell-produced IFN-gamma and TNF-alpha induce target cell cytolysis through up-regulation of ICAM-1.
    Authors: Wang R, Jaw JJ, Jaw J, Stutzman N, Stutzman NC, Zou Z, Sun PD
    J Leukoc Biol, 0;91(2):299-309.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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