抗IL-1 Receptor Antagonist抗体(Anti-IL-1 Receptor Antagonist, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL‑1ra/IL-1F3Arg26-Glu177Accession # P18510
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL‑1ra/IL-1F3 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 15% cross-reactivity with recombinant mouse IL-1ra is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Simple Western	20 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑1ra/IL‑1F3 inhibition of IL‑1 alpha /IL‑1F1-dependent proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically ≤4 µg/mL in the presence of 50 ng/mL Recombinant Human IL‑1ra/IL‑1F3 and 50 pg/mL Recombinant Human IL‑1 alpha /IL‑1F1.

Western Blot
	Detection of Human IL‑1ra/IL‑1F3 by Western Blot. Western blot shows lysates of RT-4 human bladder carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑1ra/IL‑1F3 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
	IL‑1ra/IL‑1F3 in Human Skin. IL‑1ra/IL‑1F3 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western
	Detection of Human and Mouse IL‑1ra/IL‑1F3 by Simple WesternTM. Simple Western lane view shows lysates of RT-4 human bladder carcinoma cell line, A431 human epithelial carcinoma cell line, human skin cancer tissue, and mouse skin cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for IL‑1ra/IL‑1F3 at approximately 24-26 kDa (as indicated) using 20 µg/mL of Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Neutralization
	IL‑1ra/IL‑1F3 Inhibition ofIL‑1 alpha /IL‑1F1-dependent Cell Proliferation and Neutralization by HumanIL‑1ra/IL‑1F3 Antibody. Recombinant HumanIL‑1ra/IL‑1F3 (Catalog #280‑RA) inhibits Recombinant Human IL‑1 alpha /IL‑1F1 (Catalog #200‑LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog #AR002). Inhibition of Recombinant HumanIL‑1 alpha /IL‑1F1 (50 pg/mL) activity elicited by Recombinant Human IL‑1ra/IL‑1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑1ra/IL‑1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA). The ND50 is typically ≤4 µg/mL.

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Related Product & Information

Long Name	Interleukin 1 Receptor Antagonist
Entrez Gene IDs	3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)
Background	IL-1ra/IL-1F3
background_content	Background: IL-1ra/IL-1F3 IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

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Citations

1. Intranasal Administration of Interleukin-1 Receptor Antagonist in a Transient Focal Cerebral Ischemia Rat Model
   Authors: JH Lee, EH Kam, JM Kim, SY Kim, EJ Kim, SY Cheon, BN Koo
   Biomol Ther (Seoul), 2017;25(2):149-157.
   Species: Rat
   Sample Type: Whole Tissue
   Application: IHC


2. Prostaglandin E2 Indicates Therapeutic Efficacy Of Mesenchymal Stem Cells In Experimental Traumatic Brain Injury
   Authors: DJ Kota, KS Prabhakara, N Toledano-F, D Bhattarai, Q Chen, B DiCarlo, P Smith, F Triolo, PL Wenzel, CS Cox, SD Olson
   Stem Cells, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


3. IL-1 Receptor Antagonist Chimeric Protein: Context-Specific and Inflammation-Restricted Activation.
   Authors: Rider P, Carmi Y, Yossef R, Guttman O, Eini H, Azam T, Dinarello C, Lewis E
   J Immunol, 2015;195(4):1705-12.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. IL-4 enhances IFN-lambda1 (IL-29) production by plasmacytoid DCs via monocyte secretion of IL-1Ra.
   Authors: Megjugorac NJ, Gallagher GE, Gallagher G
   Blood, 2010;115(21):4185-90.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
   Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
   Dig. Dis. Sci., 2007;52(10):2775-83.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


6. Immunolocalization of interleukin-1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies.
   Authors: Grundtman C, Salomonsson S, Dorph C, Bruton J, Andersson U, Lundberg IE
   Arthritis Rheum., 2007;56(2):674-87.
   Species: Human
   Sample Type: Whole Cells
   Application: ICC


7. P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
   Authors: Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC
   Br. J. Pharmacol., 2007;151(1):115-27.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


8. IL-22 increases the innate immunity of tissues.
   Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
   Immunity, 2004;21(2):241-54.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


9. T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
   Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
   J. Allergy Clin. Immunol., 2002;110(4):624-33.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut

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