抗TNF-α抗体(Anti-TNF-α, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human TNF-alpha Val77-Leu233Accession # P01375
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human TNF‑ alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant feline TNF‑ alpha, recombinant canine TNF‑ alpha, recombinant rhesus monkey TNF‑ alpha, and recombinant equine TNF-alpha is observed and approximately 15% cross‑reactivity with recombinant cotton rat TNF‑ alpha, recombinant mouse TNF‑ alpha, recombinant rat TNF‑ alpha, and recombinant guinea pig TNF‑ alpha  is observed and less than 5% cross‑reactivity with recombinant porcine TNF‑ alpha and recombinant bovine TNF‑ alpha  is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Immunocytochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M. L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 0.01-0.06 μg/ml in the presence of 0.75 ng/mL Recombinant Human TNF‑ alpha.

Neutralization
	Cytotoxicity Induced by TNF‑ alpha and Neutralization by Human TNF-alpha Antibody. Recombinant Human TNF‑ alpha (Catalog # 210-TA) induces cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Human TNF‑ alpha (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-210-NA). The ND50 is typically 0.01‑0.06 μg/mL.
Immunocytochemistry
	TNF‑ alpha in Human PBMCs. TNF‑ alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog #AF-210-NA) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Long Name	Tumor Necrosis Factor alpha
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Background	TNF-alpha
background_content	Background: TNF-alpha Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types. Human TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%‑92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness. Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha. TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-alpha regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-alpha is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

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Citations

1. Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies
   Authors: R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson
   Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
   Application: Affinity Assay


2. Detection of aryl hydrocarbon receptor agonists in human samples
   Authors: V Rothhammer, DM Borucki, JE Kenison, P Hewson, Z Wang, R Bakshi, DH Sherr, FJ Quintana
   Sci Rep, 2018;8(1):4970.
   Species: Human
   Sample Type: Serum
   Application: Neut


3. Promising Role of Toll-Like Receptor 8 Agonist in Concert with Prostratin for Activation of Silent HIV
   Authors: M A Rochat
   J. Virol, 2016;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Inflammatory Adipokines Decrease Expression of Two High Molecular Weight Isoforms of Tropomyosin Similar to the Change in Type 2 Diabetic Patients
   PLoS ONE, 2016;11(9):e0162908.
   Species: Human
   Sample Type: Whole Cells
   Application: Bioassay


5. Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
   Authors: Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G
   Proc Natl Acad Sci U S A, 2014;111(52):E5688-96.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
   Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
   Cytokine, 2013;64(3):660-5.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Development


7. The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes.
   Authors: Cunin P, Caillon A, Corvaisier M, Garo E, Scotet M, Blanchard S, Delneste Y, Jeannin P
   J. Immunol., 2011;186(7):4175-82.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Native IL-32 is released from intestinal epithelial cells via a non-classical secretory pathway as a membrane-associated protein.
   Authors: Hasegawa H, Thomas HJ, Schooley K, Born TL
   Cytokine, 2011;53(1):74-83.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
   Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
   Blood, 2010;116(12):2061-9.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Not specified


10. KiSS1 metastasis suppressor gene product induces suppression of tyrosine kinase receptor signaling to Akt, tumor necrosis factor family ligand expression, and apoptosis.
    Authors: Navenot JM, Fujii N, Peiper SC
    Mol. Pharmacol., 2009;75(5):1074-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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