抗IL-4抗体(Anti-IL-4, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL‑4His25-Ser153Accession # NP_000580
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant feline IL-4, recombinant canine IL-4, recombinant equine IL-4, and recombinant porcine IL-4 is observed and less than 1% cross-reactivity with recombinant mouse IL-4 and recombinant rat IL-4 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL‑4 (Catalog # 204-IL)
Neutralization	Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.02-0.1µg/mL in the presence of 0.5 ng/mL Recombinant Human IL‑4.

Neutralization

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody. Recombinant Human IL‑4 (Catalog # 204-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-204-NA). The ND50 is typically 0.02-0.1 µg/mL.

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Related Product & Information

Long Name	Interleukin 4
Entrez Gene IDs	3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Background	IL-4
background_content	Background: IL-4 Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure. Human IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities. IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma  chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma.

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Citations

1. Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
   Authors: Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
   Blood, 2010;116(12):2061-9.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC - Not specified


2. IL-10 suppresses Th17 cells and promotes regulatory T cells in the CD4+ T cell population of rheumatoid arthritis patients.
   Authors: Heo YJ, Joo YB, Oh HJ, Park MK, Heo YM, Cho ML, Kwok SK, Ju JH, Park KS, Cho SG, Park SH, Kim HY, Min JK
   Immunol. Lett., 2010;127(2):150-6.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
   Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
   Eur. J. Immunol., 2008;38(4):1012-23.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
   Authors: Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC
   Blood, 2007;109(11):4856-64.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Peripheral expansion of circulating T-helper 1 cells predicts coronary endothelial dysfunction after cardiac transplantation.
   Authors: Methe H, Wiegand D, Welsch U, Meiser B, Nabauer M, Koglin J
   J. Heart Lung Transplant., 2005;24(7):833-40.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC


6. Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression.
   Authors: Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G
   Eur. J. Immunol., 2004;34(12):3485-96.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
   Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
   J. Allergy Clin. Immunol., 2002;110(4):624-33.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut

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