抗IL-1β抗体(Anti-IL-1β, Human, Goat-Poly antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL-1 beta /IL-1F2Ala117-Ser269Accession # NP_000567
Source	Polyclonal Goat IgG
Purification	Antigen Affinity-purified
Specificity	Detects human IL-1 beta /IL-1F2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30%‑75% cross-reactivity with recombinant mouse IL‑1 beta, recombinant porcine IL-1 beta, recombinant rat IL-1 beta, recombinant canine IL-1 beta, recombinant equine IL-1 beta, recombinant cotton rat IL-1 beta, recombinant feline IL‑1 beta, recombinant rhesus monkey IL-1 beta, and recombinant guinea pig IL-1 beta  is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below
Neutralization	Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND50) is typically 0.004-0.02 µg/mL in the presence of 50 pg/mL Recombinant Human IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.

Western Blot
	Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
	IL‑1 beta /IL‑1F2 in Human PBMCs. IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-HumanIL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Neutralization
	Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral­ization by Human IL‑1 beta /IL‑1F2 Antibody. Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA). The ND50 is typically 0.004-0.02 µg/mL in the presence of concanavalin A (1.25 µg/mL).

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Related Product & Information

Long Name	Interleukin 1 beta
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Background	IL-1 beta/IL-1F2
background_content	Background: IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus monkey and 67-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

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Citations

1. An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition
   Authors: HL Young, EJ Rowling, M Bugatti, E Giurisato, N Luheshi, I Arozarena, JC Acosta, J Kamarashev, DT Frederick, ZA Cooper, A Reuben, J Gil, KT Flaherty, JA Wargo, W Vermi, MP Smith, C Wellbrock, A Hurlstone
   J. Exp. Med., 2017;0(0):.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


2. The Regulation of Uterine Proinflammatory Gene Expression during Pregnancy in the Live-Bearing Lizard, Pseudemoia entrecasteauxii
   Authors: K Hendrawan, CM Whittingto, MC Brandley, K Belov, MB Thompson
   J. Exp. Zool. B Mol. Dev. Evol, 2017;0(0):.
   Species: Lizard
   Sample Type: Tissue Homogenates
   Application: WB


3. Autophagy gene ATG7 regulates ultraviolet radiation-induced inflammation and skin tumorigenesis
   Authors: L Qiang, A Sample, CR Shea, K Soltani, KF Macleod, YY He
   Autophagy, 2017;0(0):0.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. ACN9 Regulates the Inflammatory Responses in Human Bronchial Epithelial Cells
   Authors: JH Jeong, J Kim, J Kim, HR Heo, JS Jeong, YJ Ryu, Y Hong, SS Han, SH Hong, SJ Lee, WJ Kim
   Tuberc Respir Dis (Seoul), 2017;80(3):247-254.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


5. 17-oxo-DHA displays additive anti-inflammatory effects with fluticasone propionate and inhibits the NLRP3 inflammasome
   Sci Rep, 2016;6(0):37625.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


6. Pyrin inflammasome activation and RhoA signaling in the autoinflammatory diseases FMF and HIDS
   Authors: Yong Hwan Park
   Nat Immunol, 2016;0(0):.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


7. Efficacy and Pharmacology of the NLRP3 Inflammasome Inhibitor CP-456,773 (CRID3) in Murine Models of Dermal and Pulmonary Inflammation
   J Immunol, 2016;0(0):.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: WB


8. A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases.
   Authors: Coll R, Robertson A, Chae J, Higgins S, Munoz-Planillo R, Inserra M, Vetter I, Dungan L, Monks B, Stutz A, Croker D, Butler M, Haneklaus M, Sutton C, Nunez G, Latz E, Kastner D, Mills K, Masters S, Schroder K, Cooper M, O'Neill L
   Nat Med, 2015;21(3):248-55.
   Species: Mouse
   Sample Type: Cell Lysates
   Application: WB


9. Methylsulfonylmethane inhibits NLRP3 inflammasome activation.
   Authors: Ahn H, Kim J, Lee M, Kim Y, Cho Y, Lee G
   Cytokine, 2015;71(2):223-31.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: WB


10. Acid-dependent Interleukin-1 (IL-1) Cleavage Limits Available Pro-IL-1beta for Caspase-1 Cleavage.
    Authors: Edye M, Brough D, Allan S
    J Biol Chem, 2015;290(42):25374-81.
    Species: Human
    Sample Type: Recombinant Protein
    Application: WB

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