抗Human IFN-gamma Fluorescein抗体(Anti-Human IFN-gamma Fluorescein antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Fluorescein
Immunogen	E. coli-derived recombinant human IFN-gamma Gln24-Gln166Accession # AAP20098.1
Source	Monoclonal Mouse IgG2B Clone # 25723
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IFN-gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma, recombinant rat IFN-gamma, or recombinant porcine IFN-gamma is observed.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of IFN‑ gamma in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA, 1 μg/mL Ionomycin, and 3 μM Monensin overnight were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human IFN‑ gamma Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC285F) or (B) Mouse IgG2B Fluorescein Isotype Control (Catalog # IC0041F).  To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background

IFN-gamma

background_content

Background: IFN-gamma Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta  subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

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Citations

1. B Lymphocytes in Multiple Sclerosis: Bregs and BTLA/CD272 Expressing-CD19+ Lymphocytes Modulate Disease Severity
   Sci Rep, 2016;6(0):29699.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
   Sci Rep, 2016;6(0):32320.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Expansion of anti-mesothelin specific CD4+ and CD8+ T cell responses in patients with pancreatic carcinoma.
   Authors: Chen Y, Ayaru L, Mathew S, Morris E, Pereira S, Behboudi S
   PLoS ONE, 2014;9(2):e88133.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. IL-10-producing regulatory B cells in the pathogenesis of chronic hepatitis B virus infection.
   Authors: Das, Abhishek, Ellis, Gidon, Pallant, Celeste, Lopes, A Ross, Khanna, Pooja, Peppa, Dimitra, Chen, Antony, Blair, Paul, Dusheiko, Geoffrey, Gill, Upkar, Kennedy, Patrick, Brunetto, Maurizia, Lampertico, Pietro, Mauri, Claudia, Maini, Mala K
   J Immunol, 2012;189(8):3925-35.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. NF-kappaB modulates sensitivity to apoptosis, proinflammatory and migratory potential in short- versus long-term cultured human gammadelta lymphocytes.
   Authors: Ferrarini M, Delfanti F, Gianolini M, Rizzi C, Alfano M, Lazzarin A, Biswas P
   J. Immunol., 2008;181(9):5857-64.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


6. Overlapping human leukocyte antigen class I/II binding peptide vaccine for the treatment of patients with stage IV melanoma: evidence of systemic immune dysfunction.
   Authors: Celis E, Celis E
   Cancer, 2007;110(1):203-14.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


7. Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes.
   Authors: D'Ambrosio D
   Eur. J. Immunol., 2002;32(10):2934-43.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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