抗IL-6抗体(Anti-IL-6, Human, Mouse-Mono, FITC antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Fluorescein
Immunogen	E. coli-derived recombinant human IL‑6Val30-Met212Accession # P05231
Source	Monoclonal Mouse IgG2B Clone # 1936
Purification	Protein A or G purified from ascites
Specificity	Detects human IL-6 in direct ELISAs. Does not cross-react with recombinant IL-6 from mouse, rat, or pig.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of IL‑6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with LPS were stained with Mouse Anti-Human IL‑6 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC206F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background

IL-6

background_content

Background: IL-6 Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22‑28 kDa phosphorylated and variably glycosylated molecule (1‑4). Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6 (5). Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (6‑9). Human IL-6 is equally active on mouse and rat cells (10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans‑signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3). Trans‑signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans‑signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 13).

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Citations

1. A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis.
   Authors: Gironi, Maira, Saresella, Marina, Rovaris, Marco, Vaghi, Matilde, Nemni, Raffaell, Clerici, Mario, Grossi, Enzo
   Immun Ageing, 2013;10(1):1.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Vitamin D receptor gene polymorphisms and HLA DRB1*04 cosegregation in Saudi type 2 diabetes patients.
   Authors: Al-Daghri NM, Al-Attas O, Alokail MS
   J. Immunol., 2012;188(3):1325-32.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Expression of the T cell receptor alpha/beta on a CD123+ BDCA2+ HLA-DR+ subpopulation in head and neck squamous cell carcinoma.
   Authors: Thiel A, Kesselring R, Pries R, Puzik A, Wittkopf N, Wollenberg B
   PLoS ONE, 2011;6(1):e15997.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Kinin receptor expression during Staphylococcus aureus infection.
   Authors: Bengtson SH, Phagoo SB, Norrby-Teglund A, Pahlman L, Morgelin M, Zuraw BL, Leeb-Lundberg LM, Herwald H
   Blood, 2006;108(6):2055-63.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells.
   Authors: Lenz P, Bacot SM, Frazier-Jessen MR, Feldman GM
   FEBS Lett., 2003;538(1):149-54.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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