抗IL-2抗体(Anti-IL-2, Human, Mouse-Mono, PE antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Phycoerythrin
Immunogen	E. coli-derived recombinant human IL‑2Ala21-Thr153Accession # NP_000577
Source	Monoclonal Mouse IgG1 Clone # 5334
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry
	Detection of IL‑2 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and ionomycin were stained with Mouse Anti-Human IL‑2 PE‑conjugated Monoclonal Antibody (Catalog # IC202P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Long Name	Interleukin 2
Background	IL-2
background_content	Background: IL-2 IL-2 is a 15-19 kDa secreted O-glycosylated polypeptide that belongs to the gamma c -receptor utilizing family of molecules. It is a monomer that is expressed by a limited number of cell types, including CD4+ and CD8+ T cells, gamma δ T cells, eosinophils and B cells. Its local concentration, plus the stoichiometry of it receptor ( a two or three subunit complex) appears to determine what effects it has on its target cells. It is suggested to both induce NK and CD8+ T cell proliferation, and promote NK cell and CD8+ T cell effector activity. IL-2 also appears to drive CD4+ Fox P3- thymocytes into mature FoxP3+ Tregs, and to direct the conversion of CD4+ T cells into induced Tregs. Finally, IL-2 induces gamma δ T cells to secrete IFN-gamma, and endothelial cells to upregulate endocytic activity. Human and mouse IL-2 share 56 % amino acid sequence identity.

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Citations

1. Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration.
   Authors: Arneja A, Johnson H, Gabrovsek L, Lauffenburger D, White F
   J Immunol, 2014;192(1):123-35.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Building and optimizing a virus-specific T cell receptor library for targeted immunotherapy in viral infections.
   Authors: Banu, Nasirah, Chia, Adeline, Ho, Zi Zong, Garcia, Alfonso, Paravasivam, Komathi, Grotenbreg, Gijsbert, Bertoletti, Antonio, Gehring, Adam J
   Sci Rep, 2014;4(0):4166.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. Hypoxia potentiates glioma-mediated immunosuppression.
   Authors: Wei J, Wu A, Kong LY, Wang Y, Fuller G, Fokt I, Melillo G, Priebe W, Heimberger AB
   PLoS ONE, 2012;6(1):e16195.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
   Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
   J. Immunol., 2008;180(7):5109-17.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


5. A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients.
   Authors: Hussain SF, Kong LY, Jordan J, Conrad C, Madden T, Fokt I, Priebe W, Heimberger AB
   Cancer Res., 2007;67(20):9630-6.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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