抗IL-1β抗体(Anti-IL-1β, Human, Mouse-Mono, FITC antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Fluorescein
Immunogen	E. coli-derived recombinant human IL-1 beta /IL-1F2Ala117-Ser269Accession # P01584
Source	Monoclonal Mouse IgG1 Clone # 8516
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-1 beta /IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL-1 beta /IL-1F2 and recombinant porcine IL-1 beta /IL-1F2 and no cross-reactivity with recombinant rat (rr) IL-1 beta /IL-1F2, rmIL‑1 alpha, recombinant human IL‑1ra, rmIL‑1ra, or rrIL‑1 alpha.

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Applications and Data

	Recommended Concentration	Sample
Intracellular Staining by Flow Cytometry	10 µL/106 cells	See below

Intracellular Staining by Flow Cytometry

Detection of IL‑1 beta /IL‑1F2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with (A) Mouse Anti-Human IL‑1 beta /IL‑1F2 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC201F) and Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P). Excess Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) inhibits IC201F staining (B). Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Related Product & Information

Background

IL-1 beta/IL-1F2

background_content

Background: IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R Accessory Protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

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Citations

1. Kinin receptor expression during Staphylococcus aureus infection.
   Authors: Bengtson SH, Phagoo SB, Norrby-Teglund A, Pahlman L, Morgelin M, Zuraw BL, Leeb-Lundberg LM, Herwald H
   Blood, 2006;108(6):2055-63.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells.
   Authors: Lenz P, Bacot SM, Frazier-Jessen MR, Feldman GM
   FEBS Lett., 2003;538(1):149-54.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


3. T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
   Authors: Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
   J. Allergy Clin. Immunol., 2002;110(4):624-33.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


4. Genetic control of IL-1beta bioactivity through differential regulation of the IL-1 receptor antagonist.
   Authors: Vamvakopoulos J, Green C, Metcalfe S
   Eur. J. Immunol., 2002;32(10):2988-96.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow

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