抗Human MMP-9抗体(Anti-Human MMP-9 antibody)
掲載日情報：2021/01/28 現在Webページ番号：28674
Human MMP-9に対する抗体(Anti-Human MMP-9 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- Related Research Areas
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月18日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Human MMP-9 MAb (Clone 36020) |
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Anti-Human MMP-9 MAb (Clone 36020) |
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Anti-Human MMP-9 MAb (Clone 36020) |
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[在庫・価格 ：2024年05月18日 00時00分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human MMP-9 MAb (Clone 36020)
文献数： 1
- 商品コード：MAB936-100
- メーカー：RSD
- 包装：100μg
- 価格：¥104,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
別名：92 kDa gelatinase クローン：36020 Genbank No： 4318 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human | 適用 | ELISA,IC,IHC,IP,Western Blot |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Monoclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human MMP-9 MAb (Clone 36020)
文献数： 1
- 商品コード：MAB936-500
- メーカー：RSD
- 包装：500μg
- 価格：¥232,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Human/Primate MMP-9 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF911，スタンダードとして#911-MP-010を用いる。 別名：92 kDa gelatinase クローン：36020 Genbank No： 4318 |
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| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human | 適用 | ELISA,IC,IHC,IP,Western Blot |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Monoclonal | フォーマット | |
| 掲載カタログ |
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| 製品記事 | |||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human MMP-9 MAb (Clone 36020)
文献数： 1
- 商品コード：MAB936-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。 別名：92 kDa gelatinase クローン：36020 Genbank No： 4318 |
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|---|---|---|---|
| 法規制等 | |||
| 保存条件 | -20℃ | 法規備考 | |
| 抗原種 | 免疫動物 | Mouse | |
| 交差性 | Human | 適用 | ELISA,IC,IHC,IP,Western Blot |
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified |
| 吸収処理 | クラス | IgG | |
| クロナリティ | Monoclonal | フォーマット | |
| 掲載カタログ |
|
||
| 製品記事 | |||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
||
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Product Details
| Species Reactivity | Human, Primate |
|---|---|
| Label | Unconjugated |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human MMP-9 |
| Source | Monoclonal Mouse IgG1 Clone # 36020 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human MMP-9 in Western blots. Does not react with the C-terminal truncated form (65 kDa) of recombinant human (rh) MMP-9. In Western blots, approximately 5% cross-reactivity with rhMMP-2 and no cross-reactivity with rhMMP-1 or rhMMP-3 is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Human MMP‑9 Western Blot Standard (Catalog # WBC018) |
| Immunohistochemistry | 8-25 µg/mL | See below |
| Immunoprecipitation | 25 µg/mL | Conditioned cell culture medium spiked with Recombinant Human MMP‑9 (Catalog # 911-MP), see our available Western blot detection antibodies |
| Immunocytochemistry | 3-25 µg/mL | See below |
| Human/Primate MMP-9 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human/Primate MMP‑9 Antibody (Catalog #MAB936 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human/Primate MMP‑9 Biotinylated Antibody (Catalog #BAF911 ) |
| ELISA Standard | Recombinant Human MMP-9 Protein, CF (Catalog #911-MP ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Immunocytochemistry |
![Immunocytochemistry MMP-9 Antibody (36020) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/MMP9_MAB936_Immunocytochemistry__Immunofluorescence_21488.jpg) click image to view larger | MMP‑9 in SW1353 Human Cell Line. MMP‑9 was detected in immersion fixed SW1353 human chondrosarcoma cell line using Mouse Anti-Human/Primate MMP‑9 Monoclonal Antibody (Catalog # MAB936) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Immunohistochemistry |
![Immunohistochemistry MMP-9 Antibody (36020) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/MMP9_MAB936_Immunohistochemistry_21714.jpg) click image to view larger | MMP‑9 in Human Lung. MMP‑9 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human/Primate MMP‑9 Monoclonal Antibody (Catalog # MAB936) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: MMP-9 | Pro Matrix Metalloproteinase 9 (Pro-MMP-9) is an inactive proenzyme secreted by multiple cell types. The N-terminal pro region is proteolytically removed, resulting in active MMP-9, which can degrade collagens and elastin as well as several non-ECM molecules. |
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| Long Name: | Matrix Metalloproteinase 9 | |
| Entrez Gene IDs: | 4318 (Human); 17395 (Mouse); 81687 (Rat) | |
| Alternate Names: | 92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase | |
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Related Research Areas
| Airway Inflammation and Remodeling | ||||||||||
| Alpha Granule Soluble Molecules | ||||||||||
| Cancer Biomarkers | ||||||||||
| Cell to Cell Adhesion Disassembly During EMT | ||||||||||
| Collagen Modifying Enzymes | ||||||||||
| Enzymes Secreted by Endothelial Cells | ||||||||||
| Extracellular Matrix Remodeling and Cell Migration During EMT | ||||||||||
| Gametes and Fertilization | ||||||||||
| Inflammatory Mediators | ||||||||||
| Mesenchymal Cells and EMT | ||||||||||
| Metalloproteases and Regulators | ||||||||||
| MMPs, TIMPs and Related Molecules | ||||||||||
| Molecules Secreted by VSMC | ||||||||||
| Preeclampsia | ||||||||||
| Proteolytic Degradation of Amyloid beta | ||||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|---|
 Enlarge | Very Good | ELISA | Human | Anonymous | 11/10/2017 | Image Details |
 ELISA: Human/Primate MMP-9 Antibody [MAB936]. | |||
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Summary
| Application | ELISA |
| Sample Tested | Serum and Plasma |
| Species | Human |
| Other Experimental Details | A sandwich ELISA was built using this antibody as the capture and AF911 as the detection antibody. MMP-9 was measured in human serum and plasma samples. |
| Immunocytochemistry | |
|---|---|
 | MMP‑9 in SW1353 Human Cell Line. MMP‑9 was detected in immersion fixed SW1353 human chondrosarcoma cell line using Mouse Anti-Human/Primate MMP‑9 Monoclonal Antibody (Catalog # MAB936) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
| Immunohistochemistry | |
 | MMP‑9 in Human Lung. MMP‑9 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human/Primate MMP‑9 Monoclonal Antibody (Catalog # MAB936) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents. |
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Related Product & Information
| Background | MMP-9 |
|---|---|
| background_content | Background: MMP-9 Pro Matrix Metalloproteinase 9 (Pro-MMP-9) is an inactive proenzyme secreted by multiple cell types. The N-terminal pro region is proteolytically removed, resulting in active MMP-9, which can degrade collagens and elastin as well as several non-ECM molecules. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
- Neutrophils from vasculitis patients exhibit an increased propensity for activation by anti-neutrophil cytoplasmic antibodies.
Authors: Ohlsson S, Ohlsson S, Soderberg D, Gunnarsson L, Pettersson A, Segelmark M, Hellmark T
Clin Exp Immunol, 2014;176(3):363-72.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Capture - In vitro secretion of matrix metalloprotease 9 is a prognostic marker in childhood acute lymphoblastic leukemia.
Authors: Schneider P, Costa O, Legrand E
Leuk. Res., 2010;34(1):24-31.
Species: Human
Sample Type: Whole Cells
Application: Flow - Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
Authors: Zhao Z, McCloud B, Fleming R, Klempner MS
Biochem. Biophys. Res. Commun., 2007;358(2):528-33.
Species: Human
Sample Type: Cell Lysates
Application: WB - Distinct synovial immunopathologic characteristics of juvenile-onset spondylarthritis and other forms of juvenile idiopathic arthritis.
Authors: Kruithof E, Van den Bossche V, De Rycke L, Vandooren B, Joos R, Canete JD, Tak PP, Boots AM, Veys EM, Baeten D
Arthritis Rheum., 2006;54(8):2594-604.
Species: Human
Sample Type: Whole Tissue
Application: IHC - Neutrophil-derived metalloproteinase-9 predicts healing quality after sinus surgery.
Authors: Watelet JB, Demetter P, Claeys C, Van Cauwenberge P, Cuvelier C, Bachert C
Laryngoscope, 2005;115(1):56-61.
Species: Human
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded - Increased production of matrix metalloproteinases in Helicobacter pylori-associated human gastritis.
Authors: Bergin PJ, Anders E, Sicheng W, Erik J, Jennie A, Hans L, Pierre M, Qiang PH, Marianne QJ
Helicobacter, 2004;9(0):201.
Species: Human
Sample Type: Tissue Homogenates
Application: WB
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