抗IL-6抗体(Anti-IL-6, Porcine, Mouse-Mono antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Porcine
Label	Unconjugated
Immunogen	E. coli-derived recombinant porcine IL-6Pro29-Met212Accession # P26893
Source	Monoclonal Mouse IgG1 Clone # 77830
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects porcine IL-6 in direct ELISAs and Western blots. Approximately 2% cross-reactivity with recombinant mouse (rm) IL-6, recombinant rat IL-6, and rmLIF is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Porcine IL‑6 (Catalog # 686-PI)
Immunocytochemistry	8-25 µg/mL	See below

Immunocytochemistry

IL‑6 in Porcine PBMCs. IL‑6 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Porcine IL‑6 Monoclonal Antibody (Catalog # MAB686) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Related Product & Information

Background

IL-6

background_content

Background: IL-6 Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is central to the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 61%, 42%, and 42% aa sequence identity with human, mouse, and rat IL-6 (5). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (6). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (7). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL‑6 R (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 8).

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Citations

1. Characterization of a Broadly Reactive Anti-CD40 Agonistic Monoclonal Antibody for Potential Use as an Adjuvant
   Authors: C Martin, SD Waghela, S Lokhandwal, A Ambrus, J Bray, C Vuong, V Vinodkumar, PJ Dominowski, S Rai, D Mwangi, DL Foss, W Mwangi
   PLoS ONE, 2017;12(1):e0170504.
   Species: Porcine
   Sample Type: Whole Cells
   Application: Flow


2. Dendritic Cell Sensing of Hydrophobic Di- and Triacylated Lipopeptides Self-Assembled within Synthetic Virus-like Particles
   Authors: R Sharma, A Ghasparian, JA Robinson, KC McCullough
   J. Immunol., 2017;0(0):.
   Species: Porcine
   Sample Type: Whole Cells
   Application: Functional Assay


3. Altered expression of cytokines IL-1?, IL-6, IL-8 and TNF-? in bovine follicular persistence
   Authors: AF Stassi, ME Baravalle, EM Belotti, F Rey, NC Gareis, PU Díaz, FM Rodríguez, CJ Leiva, HH Ortega, NR Salvetti
   Theriogenology, 2017;97(0):104-112.
   Species: Bovine
   Sample Type: Whole Tissue
   Application: IHC


4. Mutations in classical swine fever virus NS4B affect virulence in swine.
   Authors: Fernandez-Sainz I, Gladue DP, Holinka LG
   J. Virol., 2010;84(3):1536-49.
   Species: Porcine
   Sample Type: Whole Tissue
   Application: IHC Frozen


5. Low vasa vasorum densities correlate with inflammation and subintimal thickening: potential role in location--determination of atherogenesis.
   Authors: Gossl M, Versari D, Lerman LO, Chade AR, Beighley PE, Erbel R, Ritman EL
   Atherosclerosis, 2009;206(2):362-8.
   Species: Porcine
   Sample Type: Whole Tissue
   Application: IHC


6. Sex difference in link between interleukin-6 and stress.
   Authors: Jankord R, Turk JR, Schadt JC, Casati J, Ganjam VK, Price EM, Keisler DH, Laughlin MH
   Endocrinology, 2007;148(8):3758-64.
   Species: Porcine
   Sample Type: Tissue Homogenates
   Application: WB

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