抗Human IL-15抗体(Anti-Human IL-15 antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human, Primate
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL-15Asn49-Ser162Accession # P40933
Source	Monoclonal Mouse IgG1 Clone # 34505
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-15 in direct ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) IL-1 alpha, -1 beta, -2, -3, -4, -5, -6, -7, -8, -9, rmIL-1 alpha, -1 beta, -3, -4, -5, -6, -7, -9, or -13 is observed.

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Applications and Data

	Recommended Concentration	Sample
Human/Primate IL-15 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human/Primate IL‑15 Antibody (Catalog #MAB647 )
ELISA Detection (Matched Antibody Pair)	0.5-2.0 µg/mL	Human/Primate IL‑15 Biotinylated Antibody (Catalog #BAM247 )
ELISA Standard		Recombinant Human IL-15 Protein (Catalog #247-IL )
Neutralization	Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 4-20 ng/mL in the presence of 3 ng/mL Recombinant Human IL‑15.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody.Recombinant Human IL‑15 (Catalog # 247-ILB) induces proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL‑15 (3 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL‑15 Monoclonal Antibody (Catalog # MAB647). The ND50 is typically 4-20 ng/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-15	Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

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References

Grabstein, K. et al. (1994) Science 264:965.
Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
Giri, J.G. et al. (1995) EMBO 14:3654.
Giri, J. et al. (1994) EMBO J. 13:2822.
Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
Dubois, S. et al. (2002) Immunity 17:537.
Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
Mortier, E. et al. (2004) J. Immunol. 173:1681.
Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
Neely, G.G. et al. (2004) J. Immunol. 172:4225.
Long Name:	Interleukin 15
Entrez Gene IDs:	3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
Alternate Names:	IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15

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Related Research Areas

Show

Common gamma Chain Receptor Family
Group 1 ILCs
Isolation, Culture, and Identification of Natural Killer Cells
Macrophage Activation Markers
Molecules Secreted by VSMC
Natural Killer T (NKT) Cells
Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling Sci Rep, 2016;6(0):32320. Species: Human Sample Type: Whole Cells Application: Flow
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Excellent		ELISA	Human	Anonymous	11/20/2017			Image Details	ELISA: Human/Primate IL-15 Antibody [MAB647].

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Summary

Application	ELISA
Sample Tested	Serum and Plasma
Species	Human
Other Experimental Details	This antibody was used as the solid phase molecule in an ELISA for IL15. The BAM247 antibody was used as the liquid phase molecule.

Neutralization

Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. Recombinant Human IL‑15 (Catalog # 247-ILB) induces proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by the Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL‑15 (3 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL‑15 Monoclonal Antibody (Catalog # MAB647). The ND50 is typically 4-20 ng/mL.

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Related Product & Information

Long Name	Interleukin 15
Entrez Gene IDs	3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
Background	IL-15
background_content	Background: IL-15 Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

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Citations

1. Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling
   Sci Rep, 2016;6(0):32320.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


2. IL-15 controls T cell functions through its influence on CD30 and OX40 antigens in Celiac Disease.
   Authors: Periolo N, Guillen L, Arruvito M, Alegre N, Niveloni S, Hwang J, Bai J, Chernavsky A
   Cytokine, 2014;67(1):44-51.
   Species: Human
   Sample Type:
   


3. Epstein-Barr virus coinfection in children boosts cytomegalovirus-induced differentiation of natural killer cells.
   Authors: Saghafian-Hedengren S, Sohlberg E, Theorell J, Carvalho-Queiroz C, Nagy N, Persson J, Nilsson C, Bryceson Y, Sverremark-Ekstrom E
   J Virol, 2013;87(24):13446-55.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
   Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
   AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


5. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
   Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
   Blood, 2007;109(10):4272-9.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


6. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
   Authors: Pfleger C, Schloot N, ter Veld F
   J. Immunol. Methods, 2007;329(1):214-8.
   Species: Human
   Sample Type: Serum
   Application: Luminex Development


7. Histological characteristics and altered expression of interleukins (IL) IL-13 and IL-15 in endometria of levonorgestrel users with different uterine bleeding patterns.
   Authors: Rhoton-Vlasak A, Hardt N
   Fertil. Steril., 2005;83(3):659-65.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Paraffin-embedded


8. Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling.
   Authors: Yamasaki S, Maeda M, Ohshima K, Kikuchi M, Otsuka T, Harada M
   Leuk. Res., 2004;28(10):1023-31.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


9. Clinical improvement in chronic plaque-type psoriasis lesions after narrow-band UVB therapy is accompanied by a decrease in the expression of IFN-gamma inducers -- IL-12, IL-18 and IL-23.
   Authors: Piskin G, Tursen U, Sylva-Steenland RM, Bos JD, Teunissen MB
   Exp. Dermatol., 2004;13(12):764-72.
   Species: Human
   Sample Type: Whole Tissue
   Application: IHC Fresh/Frozen


10. NK cell activation by dendritic cells (DCs) requires the formation of a synapse leading to IL-12 polarization in DCs.
    Authors: Borg C, Jalil A, Laderach D, Maruyama K, Wakasugi H, Charrier S, Ryffel B, Cambi A, Figdor C, Vainchenker W, Galy A, Caignard A, Zitvogel L
    Blood, 2004;104(10):3267-75.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC

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