抗Human CD25/IL-2 R alpha抗体(Anti-Human CD25/IL-2 R alpha antibody)
掲載日情報：2021/01/28 現在Webページ番号：28493
Human CD25/IL-2 R alphaに対する抗体(Anti-Human CD25/IL-2 R alpha )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年07月04日 14時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Anti-Human CD25/IL-2 R alpha MAb (Clone 24204) |
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Anti-Human CD25/IL-2 R alpha MAb (Clone 24204) |
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Anti-Human CD25/IL-2 R alpha MAb (Clone 24204) |
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[在庫・価格 ：2025年07月04日 14時15分現在]
※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
Anti-Human CD25/IL-2 R alpha MAb (Clone 24204)
文献数： 0
- 商品コード：MAB623-100
- メーカー：RSD
- 包装：100μg
- 価格：¥78,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：CD25 クローン：24204 Genbank No： 3559 Protein Accession No： P01589 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,IC,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human CD25/IL-2 R alpha MAb (Clone 24204)
文献数： 0
- 商品コード：MAB623-500
- メーカー：RSD
- 包装：500μg
- 価格：¥259,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human CD25/IL-2 Rα サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF223，スタンダードとして#223-2A-005を用いる。 別名：CD25 クローン：24204 Genbank No： 3559 Protein Accession No： P01589 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,IC,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Human CD25/IL-2 R alpha MAb (Clone 24204)
文献数： 0
- 商品コード：MAB623-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥27,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：CD25 クローン：24204 Genbank No： 3559 Protein Accession No： P01589 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,IC,IHC,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
|---|---|
| Label | Unconjugated |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha Glu22-Cys213Accession # P01589 |
| Source | Monoclonal Mouse IgG1 Clone # 24204 |
| Purification | Protein A or G purified from ascites |
| Specificity | Detects human CD25/IL-2 R alpha in ELISAs and Western blots. In Western blots, detects recombinant rat CD25/IL-2 R alpha but no cross-reactivity with recombinant mouse CD25/IL-2 R alpha. In sandwich immunoassays, this antibody does not cross-react or interfere with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-2 R beta, rhIL-3 R alpha, rhIL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL-6 R, or rhgp130. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | See below |
| Immunohistochemistry | 8-25 µg/mL | See below |
| Immunocytochemistry | 8-25 µg/mL | See below |
| Human CD25/IL-2 R alpha Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human CD25/IL-2 R alpha Antibody (Catalog #MAB623 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human CD25/IL-2 R alpha Biotinylated Antibody (Catalog #BAF223 ) |
| ELISA Standard | Recombinant Human CD25/IL-2 R alpha Protein (Catalog #223-2A ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Western Blot |
![Western Blot CD25/IL-2 R alpha Antibody (24204) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/CD25_MAB623_Western_Blot_21513.jpg) click image to view larger | Detection of Recombinant Human and Rat CD25/IL-2 R alpha by Western Blot. Western blot shows 25 ng of Recombinant Human CD25/IL-2 R alpha (Catalog # 223-2A), Recombinant Mouse CD25/IL-2 R alpha (Catalog # 2438-RM) and Recombinant Rat CD25/IL-2 R alpha Fc Chimera (Catalog # 5156-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CD25/IL-2 R alpha Monoclonal Antibody (Catalog # MAB623) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD25/IL-2 R alpha at approximately 30-250 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3. |
| Immunocytochemistry |
![Immunocytochemistry CD25/IL-2 R alpha Antibody (24204) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/IL-2_R_alpha_MAB623_Immunocytochemistry_9699.jpg) click image to view larger | CD25/IL‑2 R alpha in Human PBMCs.CD25/IL‑2 R alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB623) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Upper panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Immunohistochemistry |
![Immunohistochemistry CD25/IL-2 R alpha Antibody (24204) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/IL-2_R_alpha_MAB623_Immunohistochemistry_7042.jpg) click image to view larger | CD25/IL‑2 R alpha in Human Lymph Node.CD25/IL‑2 R alpha was detected in immersion fixed frozen sections of human lymph node using 15 µg/mL Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB623) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: CD25/IL-2 R alpha | IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum. | |
| Long Name: | Interleukin 2 Receptor alpha | |
| Entrez Gene IDs: | 3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine) | |
| Alternate Names: | CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR | |
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Related Research Areas
| Acute Lymphoblastic Leukemia | ||
| Acute Myeloid Leukemia | ||
| B Cell Activation | ||
| B Cell Markers | ||
| Cancer Biomarkers | ||
| Common gamma Chain Receptor Family | ||
| Cytokines and Receptors in Allergy and Asthma | ||
| Cytokines and Receptors in Tfh Cell Differentiation and Function | ||
| Group 1 ILCs | ||
| Group 2 ILCs | ||
| Isolation, Culture, and Identification of Natural Killer Cells | ||
| LTi Cells | ||
| Lymphoma | ||
| NCR+ILC3 | ||
| NCR-ILC3 | ||
| Neuroinflammation | ||
| Preeclampsia | ||
| Receptors in the Jak/STAT Pathway | ||
| Regulatory T Cells (Tregs) | ||
| T Cell Cytokine Signaling | ||
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| Western Blot | |
|---|---|
 | Detection of Recombinant Human and Rat CD25/IL-2 R alpha by Western Blot. Western blot shows 25 ng of Recombinant Human CD25/IL-2 R alpha (Catalog # 223-2A), Recombinant Mouse CD25/IL-2 R alpha (Catalog # 2438-RM) and Recombinant Rat CD25/IL-2 R alpha Fc Chimera (Catalog # 5156-RM). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human CD25/IL-2 R alpha Monoclonal Antibody (Catalog # MAB623) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD25/IL-2 R alpha at approximately 30-250 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3. |
| Immunocytochemistry | |
 | CD25/IL‑2 R alpha in Human PBMCs. CD25/IL‑2 R alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB623) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Upper panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
| Immunohistochemistry | |
 | CD25/IL‑2 R alpha in Human Lymph Node. CD25/IL‑2 R alpha was detected in immersion fixed frozen sections of human lymph node using 15 µg/mL Mouse Anti-Human CD25/IL‑2 R alpha Monoclonal Antibody (Catalog # MAB623) overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
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Related Product & Information
| Background | CD25/IL-2 R alpha |
|---|---|
| background_content | Background: CD25/IL-2 R alpha IL-2 R alpha is a type I transmembrane protein that associates with IL-2 R beta and the common gamma chain to form a high affinity heterotrimeric receptor complex for IL-2. A soluble form of IL-2 R alpha has been detected in serum. |
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Citations
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
- Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009;87(12):1814-20.
Species: Human
Sample Type: Urine
Application: Antibody Array Development - Imbalance in the peritoneal levels of interleukin 1 and its decoy inhibitory receptor type II in endometriosis women with infertility and pelvic pain.
Authors: Akoum A, Al-Akoum M, Lemay A, Maheux R, Leboeuf M
Fertil. Steril., 2007;89(6):1618-24.
Species: Human
Sample Type: Peritoneal Fluid
Application: ELISA Development - Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor.
Authors: Wang P, Zhang J, Bian H, Wu P, Kuvelkar R, Kung TT, Crawley Y, Egan RW, Billah MM
Biochem. J., 2004;380(0):425-33.
Species: Human
Sample Type:
Application: WB
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製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
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