抗Human GM-CSF抗体(Anti-Human GM-CSF antibody)

• 価格
• Product Details
• Applications and Data
• Related Research Areas
• Summary
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human GM-CSFAla18-Glu144Accession # P04141
Source	Monoclonal Mouse IgG1 Clone # 6804
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human GM-CSF in ELISAs. In ELISAs, no cross-reactivity with recombinant mouse GM‑CSF, recombinant rat GM‑CSF, recombinant human (rh) M‑CSF, rhG‑CSF, rhIL‑5‑R alpha, or rhIL‑3 R alpha is observed.

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Applications and Data

	Recommended Concentration	Sample
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	2.5 µg/106 cells	Human mature dendritic cells treated with LPS, Recombinant Human TNF‑ alpha (Catalog # 210‑TA), and Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) fixed with paraformaldehyde and permeabilized with saponin
Human GM-CSF Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human GM‑CSF Antibody (Catalog #MAB615 )
ELISA Detection (Matched Antibody Pair)	0.5-2.0 µg/mL	Human GM‑CSF Biotinylated Antibody (Catalog #BAM215 )
ELISA Standard		Recombinant Human GM-CSF Protein (Catalog #215-GM )
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.		Preparation and Storage
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GM-CSF	GM-CSF is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta  chain that is shared by the high affinity receptors for IL-3 and IL-5.
Long Name:	Granulocyte Macrophage Growth Factor
Entrez Gene IDs:	1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
Alternate Names:	colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; molgramostin; sargramostim

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Related Research Areas

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Common beta Chain Receptor Family
Cytokines and Receptors in Allergy and Asthma
Cytokines and Receptors in Angiogenesis
Dendritic Cell Development
Gametes and Fertilization
gamma delta T Cells
Inflammatory Mediators
Isolation, Culture, and Identification of Natural Killer Cells
Macrophage Activation Markers
Myeloid-derived Suppressor Cells (MDSC)
Natural Killer T (NKT) Cells
T Cell Cytokine Signaling
Azithromycin decreases NALP3 mRNA stability in monocytes to limit inflammasome-dependent inflammation Authors: EA Lendermon, TA Coon, JS Bednash, NM Weathingto, JF McDyer, RK Mallampall Respir. Res., 2017;18(1):131. Species: Human Sample Type: Cell Lysates Application: WB
Images	Ratings	Applications	Species	Reviewed By	Date	Details
	Very Good		Affinity Purification	Hamster	Anonymous	12/13/2017

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Summary

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Application	Affinity Purification
Sample Tested	CHO Chinese hamster ovary cell line
Species	Hamster
Enlarge	Very Good		ELISA	Human	Anonymous	11/02/2017			Image Details	ELISA: Human GM-CSF Antibody [MAB615].

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Summary

Application	ELISA
Sample Tested	Serum and Plasma
Species	Human
Other Experimental Details	This antibody was used as a capture in developing a sandwich ELISA for measuring GM-CSF in human serum and plasma. BAM215 paired very well with this antibody.

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Related Product & Information

Background	GM-CSF
background_content	Background: GM-CSF GM-CSF is a growth factor produced by a variety of lymphoid cells. It binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta  chain that is shared by the high affinity receptors for IL-3 and IL-5.

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Citations

1. Azithromycin decreases NALP3 mRNA stability in monocytes to limit inflammasome-dependent inflammation
   Authors: EA Lendermon, TA Coon, JS Bednash, NM Weathingto, JF McDyer, RK Mallampall
   Respir. Res., 2017;18(1):131.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


2. A multiplex immunoassay for human adipokine profiling.
   Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
   Clin. Chem., 2010;56(0):1320.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


3. Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
   Authors: Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
   AIDS Res. Hum. Retroviruses, 2008;24(3):453-62.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


4. Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
   Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
   Blood, 2007;109(10):4272-9.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: Luminex Development


5. Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
   Authors: Hirst SJ, Hallsworth MP, Peng Q
   Am. J. Respir. Crit. Care Med., 2002;165(8):1161-71.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development

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