抗Human IL-1 beta/IL-1F2抗体(Anti-Human IL-1 beta/IL-1F2 antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	E. coli-derived recombinant human IL-1 beta /IL-1F2
Source	Monoclonal Mouse IgG1 Clone # 2805
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-1 beta /IL-1F2 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 4% cross-reactivity with recombinant rat (rr) IL‑1 beta and less than 0.1% with recombinant porcine (rp) IL-1 beta, recombinant human IL-1 alpha, rpIL-1 alpha, rrIL-1 alpha, recombinant mouse (rm) IL-1 alpha, and rmIL-1 beta is observed.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Immunocytochemistry	8-25 µg/mL	See below
Human IL-1 beta /IL-1F2 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human IL‑1 beta /IL‑1F2 Antibody (Catalog #MAB601 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog #BAF201 )
ELISA Standard		Recombinant Human IL-1 beta/IL-1F2 Protein (Catalog #201-LB )
Neutralization	Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 0.05-0.2 µg/mL in the presence of 0.05 ng/mL Recombinant Human IL‑1 beta /IL‑1F2 and 1.25 µg/mL concanavalin A.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

目次に戻る

Data Examples

Western Blot	click image to view larger	Detection of Human IL‑1 beta /IL‑1F2 by Western Blot.Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Neutralization	click image to view larger	Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 beta /IL‑1F2 Antibody.Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑1 beta /IL‑1F2 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601). The ND50 is typically 0.05-0.2 µg/mL in the presence of concanavalin A (1.25 µg/mL).
Immunocytochemistry	click image to view larger	IL‑1 beta /IL‑1F2 in Human PBMCs. IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2	IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

目次に戻る

References

Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
March, C.J. et al. (1985) Nature 315:641.
Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. USA 81:7907.
Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Long Name:	Interleukin 1 beta
Entrez Gene IDs:	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names:	catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

目次に戻る

Related Research Areas

Alpha Granule Soluble Molecules

Cancer Biomarkers

Conventional/Classical Dendritic Cells

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

IL-1 Family

Infectious Diseases

Inflammatory Mediators

Isolation, Culture, and Identification of Natural Killer Cells

LTi Cells

Macrophage Activation Markers

Multiple Sclerosis and Myelin Related Molecules

Myeloid-derived Suppressor Cells (MDSC)

NCR-ILC3

Neuroinflammation

NOD-like Receptors and the Inflammasome

Regulatory T Cells (Tregs)

T Cell Cytokine Signaling

Th17 Cells

Th9 Cells

Targeting the miR-665-3p-ATG4B-autophagy axis relieves inflammation and apoptosis in intestinal ischemia/reperfusion Authors: Z Li, G Wang, D Feng, G Zu, Y Li, X Shi, Y Zhao, H Jing, S Ning, W Le, J Yao, X Tian Cell Death Dis, 2018;9(5):483. Species: Human Sample Type: Tissue Lysates Application: WB

Western Blot
	Detection of Human IL‑1 beta /IL‑1F2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Neutralization
	Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by HumanIL‑1 beta /IL‑1F2 Antibody. Recombinant HumanIL‑1 beta /IL‑1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑1 beta /IL‑1F2 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601). The ND50 is typically 0.05-0.2 µg/mL in the presence of concanavalin A (1.25 µg/mL).
Immunocytochemistry
	IL‑1 beta /IL‑1F2 in Human PBMCs. IL‑1 beta /IL‑1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-HumanIL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

目次に戻る

Related Product & Information

Long Name	Interleukin 1 beta
Entrez Gene IDs	3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Background	IL-1 beta/IL-1F2
background_content	Background: IL-1 beta/IL-1F2 IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

目次に戻る

Citations

1. Targeting the miR-665-3p-ATG4B-autophagy axis relieves inflammation and apoptosis in intestinal ischemia/reperfusion
   Authors: Z Li, G Wang, D Feng, G Zu, Y Li, X Shi, Y Zhao, H Jing, S Ning, W Le, J Yao, X Tian
   Cell Death Dis, 2018;9(5):483.
   Species: Human
   Sample Type: Tissue Lysates
   Application: WB


2. Innate activation of human primary epithelial cells broadens the host response to Mycobacterium tuberculosis in the airways
   Authors: AK Reuschl, MR Edwards, R Parker, DW Connell, L Hoang, A Halliday, H Jarvis, N Siddiqui, C Wright, S Bremang, SM Newton, P Beverley, RJ Shattock, OM Kon, A Lalvani
   PLoS Pathog., 2017;13(9):e1006577.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Zinc inhibits high glucose-induced NLRP3 inflammasome activation in human peritoneal mesothelial cells
   Authors: Y Fan, X Zhang, L Yang, J Wang, Y Hu, A Bian, J Liu, J Ma
   Mol Med Rep, 2017;0(0):.
   Species: Human
   Sample Type: Cell Lysates
   Application: WB


4. Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
   Authors: Sommer A, Fabri M
   PLoS ONE, 2015;10(6):e0130395.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Alpha 1-antitrypsin does not inhibit human monocyte caspase-1.
   Authors: Rahman M, Mitra S, Sarkar A, Wewers M
   PLoS ONE, 2015;10(2):e0117330.
   Species: Human
   Sample Type: Plasma
   Application: ELISA Capture


6. Inflammasome priming by lipopolysaccharide is dependent upon ERK signaling and proteasome function.
   Authors: Ghonime M, Shamaa O, Das S, Eldomany R, Fernandes-Alnemri T, Alnemri E, Gavrilin M, Wewers M
   J Immunol, 2014;192(8):3881-8.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


7. IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.
   Authors: Ramirez-Carrozzi V, Sambandam A, Luis E
   Nat. Immunol., 2011;12(12):1159-66.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


8. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
   Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
   J. Immunol., 2009;183(9):5799-806.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


9. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
   Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
   Transplantation, 2009;87(12):1814-20.
   Species: Human
   Sample Type: Urine
   Application: Antibody Array Development


10. Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines.
    Authors: Mitchell CM, Balkus J, Agnew KJ, Cohn S, Luque A, Lawler R, Coombs RW, Hitti JE
    AIDS Res. Hum. Retroviruses, 2008;24(5):667-71.
    Species: Human
    Sample Type: Vaginal Fluid
    Application: ELISA Development

目次に戻る