抗Mouse CCL17/TARC抗体(Anti-Mouse CCL17/TARC antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived Recombinant Mouse CCL17/TARCAla24-Pro93Accession # Q9WUZ6
Source	Monoclonal Rat IgG2A Clone # 110904
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse CCL17/TARC in ELISAs.

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Applications and Data

	Recommended Concentration	Sample
Mouse CCL17/TARC Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse CCL17/TARC Antibody (Catalog #MAB529 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse CCL17/TARC Biotinylated Antibody (Catalog #BAF529 )
ELISA Standard		Recombinant Mouse CCL17/TARC Protein (Catalog #529-TR )
Neutralization	Measured by its ability to neutralize CCL17/TARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.75-3.0 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL17/TARC.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Chemotaxis Induced by CCL17/TARC and Neutralization by Mouse CCL17/TARC Antibody.Recombinant Mouse CCL17/TARC (Catalog # 529-TR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL17/TARC (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL17/TARC Monoclonal Antibody (Catalog # MAB529). The ND50 is typically 0.75-3.0 µg/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL17/TARC	Human thymus and activation-regulated chemokine (TARC) also known as CCL17, is a novel CC chemokine identified using a signal sequence trap method. Mouse CCL17 was discovered as a dendritic cell (DC) specific gene by differentiation RNA display. Mouse CCL17 cDNA encodes a highly basic 93 amino acid (aa) residue precursor protein with a 23 aa residue putative signal peptide that is cleaved to generate the 70 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3, and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Mouse CCL17 is constitutively expressed in thymic DC, and at a lower level in lymph node DC in the lung. Recombinant CCL17 has been shown to be chemotactic for T cell lines and antigen-primed T helper cells. In humans, CCL17 was identified to be a specific functional ligand for CCR4 and CCR8, receptors that are selectively expressed on T cells.

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References

Imai, T. et al. (1997) J. Biol. Chem. 272:15036.
Imai, T. et al. (1996) J. Biol. Chem. 271:21514.
Nomiyama, H. et al. (1997) Genomics 40:211.
Lieberam, I. et al. (1999) Eur. J. Immunol. 29:2684.
Entrez Gene IDs:	6361 (Human); 20295 (Mouse)
Alternate Names:	ABCD-2; CC chemokine TARC; C-C motif chemokine 17; CCL17; chemokine (C-C motif) ligand 17; MGC138273; SCYA17MGC138271; small inducible cytokine subfamily A (Cys-Cys), member 17; Small-inducible cytokine A17; T cell-directed CC chemokine; TARC; TARCA-152E5.3; Thymus and activation-regulated chemokine

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Related Research Areas

CC Chemokines and Receptors

Chemokines and Receptors

Chemokines and Receptors in Allergy and Asthma

Macrophage Activation Markers

Regulatory T Cells (Tregs)

Th9 Cells

TNF and granulocyte macrophage-colony stimulating factor interdependence mediates inflammation via CCL17 Authors: AD Cook, MC Lee, R Saleh, HW Khiew, AD Christense, A Achuthan, AJ Fleetwood, DC Lacey, JE Smith, I Förster, JA Hamilton JCI Insight, 2018;3(6):. Species: Mouse Sample Type: In Vivo Application: Neut

Neutralization

Chemotaxis Induced by CCL17/TARC and Neutralization by Mouse CCL17/TARC Antibody. Recombinant Mouse CCL17/TARC (Catalog # 529-TR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL17/TARC (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CCL17/TARC Monoclonal Antibody (Catalog # MAB529). The ND50 is typically 0.75-3.0 µg/mL.

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Related Product & Information

Entrez Gene IDs	6361 (Human); 20295 (Mouse)
Background	CCL17/TARC
background_content	Background: CCL17/TARC Human thymus and activation-regulated chemokine (TARC) also known as CCL17, is a novel CC chemokine identified using a signal sequence trap method. Mouse CCL17 was discovered as a dendritic cell (DC) specific gene by differentiation RNA display. Mouse CCL17 cDNA encodes a highly basic 93 amino acid (aa) residue precursor protein with a 23 aa residue putative signal peptide that is cleaved to generate the 70 aa residue mature secreted protein. Among CC chemokine family members, CCL17 has approximately 24-29% amino acid sequence identity with RANTES, MIP-1 alpha, MIP-1 beta, MCP-1, MCP-2, MCP-3, and I-309. The gene for human CCL17 has been mapped to chromosome 16q13 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Mouse CCL17 is constitutively expressed in thymic DC, and at a lower level in lymph node DC in the lung. Recombinant CCL17 has been shown to be chemotactic for T cell lines and antigen-primed T helper cells. In humans, CCL17 was identified to be a specific functional ligand for CCR4 and CCR8, receptors that are selectively expressed on T cells.

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Citations

1. TNF and granulocyte macrophage-colony stimulating factor interdependence mediates inflammation via CCL17
   Authors: AD Cook, MC Lee, R Saleh, HW Khiew, AD Christense, A Achuthan, AJ Fleetwood, DC Lacey, JE Smith, I Förster, JA Hamilton
   JCI Insight, 2018;3(6):.
   Species: Mouse
   Sample Type: In Vivo
   Application: Neut


2. CCL17 blockade as a therapy for osteoarthritis pain and disease
   Authors: MC Lee, R Saleh, A Achuthan, AJ Fleetwood, I Förster, JA Hamilton, AD Cook
   Arthritis Res. Ther., 2018;20(1):62.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


3. RNA Aptamers Recognizing Murine CCL17 Inhibit T Cell Chemotaxis and Reduce Contact Hypersensitivity In�Vivo
   Authors: L Fülle, N Steiner, M Funke, F Gondorf, F Pfeiffer, J Siegl, FV Opitz, SK Ha beta el, AB Erazo, O Schanz, HJ Stunden, M Blank, C Gröber, K Händler, M Beyer, H Weighardt, E Latz, JL Schultze, G Mayer, I Förster
   Mol. Ther., 2017;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


4. iNKT Cell Emigration out of the Lung Vasculature Requires Neutrophils and Monocyte-Derived Dendritic Cells in Inflammation
   Cell Rep, 2016;16(12):3260-72.
   


5. Granulocyte macrophage colony-stimulating factor induces CCL17 production via IRF4 to mediate inflammation
   J Clin Invest, 2016;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


6. Engagement of two distinct binding domains on CCL17 is required for signaling through CCR4 and establishment of localized inflammatory conditions in the lung.
   Authors: Santulli-Marotto S, Boakye K, Lacy E, Wu S, Luongo J, Kavalkovich K, Coelho A, Hogaboam C, Ryan M
   PLoS ONE, 2013;8(12):e81465.
   Species: Mouse
   Sample Type: Recombinant Protein
   Application: DirELISA


7. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


8. CCL17 controls mast cells for the defense against filarial larval entry.
   Authors: Specht S, Frank JK, Alferink J, Dubben B, Layland LE, Denece G, Bain O, Forster I, Kirschning CJ, Martin C, Hoerauf A
   J. Immunol., 2011;186(8):4845-52.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


9. Resolution of allergic inflammation and airway hyperreactivity is dependent upon disruption of the T1/ST2-IL-33 pathway.
   Authors: Kearley J, Buckland KF, Mathie SA, Lloyd CM
   Am. J. Respir. Crit. Care Med., 2009;179(9):772-81.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


10. IL-12 produced by dendritic cells augments CD8+ T cell activation through the production of the chemokines CCL1 and CCL17.
    Authors: Henry CJ, Ornelles DA, Mitchell LM, Brzoza-Lewis KL, Hiltbold EM
    J. Immunol., 2008;181(12):8576-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut

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