抗Human ST2/IL-1 R4抗体(Anti-Human ST2/IL-1 R4 antibody)
掲載日情報：2021/01/28 現在Webページ番号：28424
Human ST2/IL-1 R4に対する抗体(Anti-Human ST2/IL-1 R4 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年06月01日 00時00分現在]
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Anti-ST2/IL-33 R, Human, Mouse-Mono(97203) |
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Anti-ST2/IL-33 R, Human, Mouse-Mono(97203) |
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Anti-ST2/IL-33 R, Human, Mouse-Mono(97203) |
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[在庫・価格 ：2025年06月01日 00時00分現在]
Anti-ST2/IL-33 R, Human, Mouse-Mono(97203)
文献数： 1
- 商品コード：MAB523-100
- メーカー：RSD
- 包装：100μg
- 価格：¥103,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：DER4 クローン：97203 Genbank No： 9173 Protein Accession No： BAA02233 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-ST2/IL-33 R, Human, Mouse-Mono(97203)
文献数： 1
- 商品コード：MAB523-500
- メーカー：RSD
- 包装：500μg
- 価格：¥232,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Human ST2/IL-1 R4 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF523，スタンダードとして#523-ST-100を用いる。 別名：DER4 クローン：97203 Genbank No： 9173 Protein Accession No： BAA02233 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | Helper T Cells Marker Antibodies |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-ST2/IL-33 R, Human, Mouse-Mono(97203)
文献数： 1
- 商品コード：MAB523-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：DER4 クローン：97203 Genbank No： 9173 Protein Accession No： BAA02233 |
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|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Human | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | Helper T Cells Marker Antibodies 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Human |
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| Label | Unconjugated |
| Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ST2/IL-33 RLys19-Phe328Accession # BAA02233 |
| Source | Monoclonal Mouse IgG1 Clone # 97203 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects human ST2/IL-33 R in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse ST2L, recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-1 RA, rhIL-1 alpha, or rhIL-1 beta is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Human ST2/IL‑1 R4 Fc Chimera (Catalog # 523-ST) |
| Human/Primate ST2/IL-33 R Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Human/Primate ST2/IL-33 R Antibody (Catalog #MAB523 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Human/Primate ST2/IL-33 R Biotinylated Antibody (Catalog #BAF523 ) |
| ELISA Standard | Recombinant Human ST2/IL-33 R Fc Chimera Protein, CF (Catalog #523-ST ) | |
| Neutralization | Measured by its ability to neutralize IL‑33-induced IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC).The Neutralization Dose (ND50) is typically 0.3-1.5 μg/mL in the presence of 1 ng/mL Recombinant Human IL‑33. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
![Neutralization ST2/IL-1 R4 Antibody (97203) [Unconjugated]](http://resources.rndsystems.com/images/datasheets/antibody/ST2_MAB523_Block_Neutralize_10981.jpg) click image to view larger | IFN-gamma Secretion Induced by IL‑33 and Neutralization by Human ST2/IL-33 R Antibody. Recombinant Human IL‑33 induces IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC) in the presence of Recombinant Human IL-12 (Catalog # 219-IL) in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Q-kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by IL‑33 is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate ST2/IL-33 R Monoclonal Antibody (Catalog # MAB523). The ND50 is typically 0.3-1.5 μg/mL. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: ST2/IL-33 R | ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks the ability of IL‑33 to signal through transmembrane ST2 (10, 13‑15). |
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References
| Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217. | |
| Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31. | |
| Tominaga, S. et al. (1992) Biochim. Biophys. Acta 1171:215. | |
| Li, H. et al. (2000) Genomics 67:284. | |
| Schmitz, J. et al. (2005) Immunity 23:479. | |
| Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979. | |
| Brint, E.K. et al. (2004) Nat. Immunol. 5:373. | |
| Weinberg, E.O. et al. (2002) Circulation 106:2961. | |
| Sanada S. et al. (2007) J. Clin. Invest. 117:1538. | |
| Palmer, G. et al. (2008) Cytokine 42:358. | |
| Chackerian, A.A. et al. (2007) J. Immunol. 179:2551. | |
| Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051. | |
| Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723. | |
| Miller, A.M. et al. (2008) J. Exp. Med. 205:339. | |
| Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369. | |
| Long Name: | Interleukin 33 Receptor |
| Entrez Gene IDs: | 9173 (Human); 17082 (Mouse) |
| Alternate Names: | DER4; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33R; Ly84; ST2; ST2L; ST2V; suppression of tumorigenicity 2; T1 |
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Related Research Areas
| Group 2 ILCs | ||
| IL-1 Family | ||
| Th2 Cells | ||
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| Neutralization | |
|---|---|
 | IFN-gamma Secretion Induced byIL‑33 and Neutralization by Human ST2/IL-33 R Antibody. Recombinant Human IL‑33 induces IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC) in the presence of Recombinant Human IL-12 (Catalog # 219-IL) in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Q-kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by IL‑33 is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate ST2/IL-33 R Monoclonal Antibody (Catalog # MAB523). The ND50 is typically 0.3-1.5 μg/mL. |
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Related Product & Information
| Long Name | Interleukin 33 Receptor |
|---|---|
| Entrez Gene IDs | 9173 (Human); 17082 (Mouse) |
| Background | ST2/IL-33 R |
| background_content | Background: ST2/IL-33 R ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks the ability of IL‑33 to signal through transmembrane ST2 (10, 13‑15). |
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Citations
- Interleukin-33 Predicts Poor Prognosis and Promotes Renal Cell Carcinoma Cell Growth Through its Receptor ST2 and the JNK Signaling Pathway
Authors: CW Wu, YG Wu, C Cheng, ZD Hong, ZM Shi, SQ Lin, J Li, XY He, AY Zhu
Cell. Physiol. Biochem., 2018;47(1):191-200.
Species: Human
Sample Type: Whole Cells
Application: Neut - A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis
Authors: D Díaz-Jimén, L Núñez, M De la Fuen, K Dubois-Cam, H Sepúlveda, M Montecino, A Torres-Riq, P García-Gon, J Chnaiderma, A Vossenkamp, TT MacDonald, D Simian, MJ González, JA Cidlowski, R Quera, MA Hermoso
Sci Rep, 2017;7(1):10180.
Species: Human
Sample Type: Whole Tissue
Application: IHC-P - The role of IL-33 and its receptor ST2 in human nasal epithelium with allergic rhinitis.
Authors: Kamekura R, Kojima T, Takano K, Go M, Sawada N, Himi T
Clin. Exp. Allergy, 2012;42(2):218-28.
Species: Human
Sample Type: Whole Cells
Application: Neut - Daily exposure to dust alters innate immunity.
Authors: Sahlander K, Larsson K, Palmberg L
PLoS ONE, 2012;7(2):e31646.
Species: Human
Sample Type: Whole Cells
Application: Neut - IL-1 Family Members IL-18 and IL-33 Upregulate the Inflammatory Potential of Differentiated Human Th1 and Th2 Cultures.
Authors: Blom L, Poulsen L
J Immunol, 2012;189(9):4331-7.
Species: Human
Sample Type: Whole Cells
Application: Neut - Effect of ovarian steroids on gene expression profile in human uterine microvascular endothelial cells.
Authors: Yasuo T, Kitaya K
Fertil. Steril., 2009;92(2):709-21.
Species: Human
Sample Type: Cell Lysates
Application: WB
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