抗GM-CSF抗体(Anti-GM-CSF, Rat, Mouse-Mono antibody)
掲載日情報：2021/01/28 現在Webページ番号：28421
GM-CSFに対する抗体(Anti-GM-CSF, Rat, Mouse-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年04月26日 20時55分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Anti-GM-CSF, Rat, Mouse-Mono(83329) |
|
1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
Anti-Rat GM-CSF MAb (Clone 83329) |
|
0 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2025年04月26日 20時55分現在]
Anti-GM-CSF, Rat, Mouse-Mono(83329)
文献数： 1
- 商品コード：MAB518
- メーカー：RSD
- 包装：500μg
- 価格：¥122,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Rat GM-CSF サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF518，スタンダードとして#518-GM-005を用いる。 別名：colony stimulating factor 2 (granulocyte-macrophage) クローン：83329 Genbank No： 1437 Protein Accession No： P48750 |
||||||
|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Rat | 免疫動物 | Mouse | ||||
| 交差性 | Rat | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
||||||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
||||||
Anti-Rat GM-CSF MAb (Clone 83329)
文献数： 0
- 商品コード：MAB518-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：colony stimulating factor 2 (granulocyte-macrophage) クローン：83329 Genbank No： 1437 Protein Accession No： P48750 |
||||||
|---|---|---|---|---|---|---|---|
| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Mouse | |||||
| 交差性 | Rat | 適用 | ELISA,Neutralising | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
||||||
| 製品記事 | M1/M2 Macrophage Activation Marker 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） 使いっきり抗体 |
||||||
| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
||||||
追加しました。
Product Details
| Species Reactivity | Rat |
|---|---|
| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant rat GM-CSFAla18-Lys144Accession # P48750 |
| Source | Monoclonal Mouse IgG2A Clone # 83329 |
| Purification | Protein A or G purified from ascites |
| Specificity | Detects rat GM-CSF in ELISAs. |
追加しました。
Applications and Data
| Recommended Concentration | Sample | |
| Rat GM-CSF Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Rat GM‑CSF Antibody (Catalog #MAB518 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Rat GM-CSF Biotinylated Antibody (Catalog #BAF518 ) |
| ELISA Standard | Recombinant Rat GM-CSF Protein (Catalog #518-GM ) | |
| Neutralization | Measured by its ability to neutralize GM‑CSF-induced proliferation in the DA3 mouse myeloma cell line. Ihle, J.N. et al. (1984) Advances in Viral Oncology. In G. Klein (eds): Raven Press, New York, NY. 4:95. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 0.5 ng/mL Recombinant Rat GM‑CSF. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
追加しました。
Data Examples
| Neutralization |
 click image to view larger | Cell Proliferation Induced by GM‑CSF and Neutralization by Rat GM‑CSF Antibody.Recombinant Rat GM‑CSF (Catalog # 518-GM) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat GM‑CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat GM‑CSF Monoclonal Antibody (Catalog # MAB518). The ND50 is typically 0.2-0.8 µg/mL. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: GM-CSF | GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL‑3 and IL‑5, is a cytokine with a core of four bundled alpha ‑helices (8-10). Mature rat GM-CSF shares 56-69% amino acid sequence identity with canine, feline, human, mouse, and porcine GM‑CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM‑CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM‑CSF R alpha (13). Rat GM‑CSF is active on mouse cells, although mouse GM‑CSF is only weakly active on rat cells (14, 15). |
|
追加しました。
References
| Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653. | |
| Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509. | |
| Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163. | |
| Cao, Y. (2007) J. Clin. Invest. 117:2362. | |
| Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405. | |
| Heuser, M. et al. (2007) Semin. Hematol. 44:148. | |
| Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321. | |
| Kaushansky, K. et al. (1992) Biochemistry 31:1881. | |
| Diederichs, K. et al. (1991) Science 254:1779. | |
| Smith, L.R. et al. (1994) Immunogenetics 39:80. | |
| Onetto-Pothier, N. et al. (1990) Blood 75:59. | |
| Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655. | |
| Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483. | |
| Oaks, M.K. et al. (1995) J. Interferon Cytokine Res. 15:1095. | |
| Vandenabeele, P. et al. (1990) Lymphokine Res. 9:381. | |
| Long Name: | Granulocyte Macrophage Growth Factor |
| Entrez Gene IDs: | 1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline) |
| Alternate Names: | colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; molgramostin; sargramostim |
追加しました。
Related Research Areas
| Common beta Chain Receptor Family | ||
| Cytokines and Receptors in Allergy and Asthma | ||
| Cytokines and Receptors in Angiogenesis | ||
| Dendritic Cell Development | ||
| Gametes and Fertilization | ||
| gamma delta T Cells | ||
| Inflammatory Mediators | ||
| Isolation, Culture, and Identification of Natural Killer Cells | ||
| Macrophage Activation Markers | ||
| Myeloid-derived Suppressor Cells (MDSC) | ||
| Natural Killer T (NKT) Cells | ||
| T Cell Cytokine Signaling | ||
| ||
| Neutralization | |
|---|---|
 | Cell Proliferation Induced by GM‑CSF and Neutralization by Rat GM‑CSF Antibody. Recombinant Rat GM‑CSF (Catalog # 518-GM) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat GM‑CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat GM‑CSF Monoclonal Antibody (Catalog # MAB518). The ND50 is typically 0.2-0.8 µg/mL. |
追加しました。
Related Product & Information
| Long Name | Granulocyte Macrophage Growth Factor |
|---|---|
| Entrez Gene IDs | 1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline) |
| Background | GM-CSF |
| background_content | Background: GM-CSF GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL‑3 and IL‑5, is a cytokine with a core of four bundled alpha ‑helices (8-10). Mature rat GM-CSF shares 56-69% amino acid sequence identity with canine, feline, human, mouse, and porcine GM‑CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM‑CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM‑CSF R alpha (13). Rat GM‑CSF is active on mouse cells, although mouse GM‑CSF is only weakly active on rat cells (14, 15). |
追加しました。
Citations
- Antibody to granulocyte macrophage colony-stimulating factor reduces the number of activated tissue macrophages and improves left ventricular function after myocardial infarction in a rat coronary artery ligation model.
Authors: Kellar RS, Lancaster JJ, Thai HM, Juneman E, Johnson NM, Byrne HG, Stansifer M, Arsanjani R, Baer M, Bebbington C, Flashner M, Yarranton G, Goldman S
J. Cardiovasc. Pharmacol., 2011;57(5):568-74.
Species: Rat
Sample Type: In Vivo
Application: In vivo
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。