抗Mouse IL-12/IL-23 p40抗体(Anti-Mouse IL-12/IL-23 p40 antibody)
掲載日情報:2021/01/28 現在Webページ番号:28406
Mouse IL-12/IL-23 p40に対する抗体(Anti-Mouse IL-12/IL-23 p40 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 :2025年05月01日 00時00分現在]
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Anti-Mouse IL-12/IL-23 p40 MAb (Clone 30517) |
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Anti-Mouse IL-12/IL-23 p40 MAb (Clone 30517) |
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Anti-Mouse IL-12/IL-23 p40 MAb (Clone 30517) |
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[在庫・価格 :2025年05月01日 00時00分現在]
Anti-Mouse IL-12/IL-23 p40 MAb (Clone 30517)
文献数: 0
- 商品コード:MAB499-100
- メーカー:RSD
- 包装:100μg
- 価格:¥90,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | クローン:30517 Genbank No: 16160 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | 法規備考 | ||||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse IL-12/IL-23 p40 MAb (Clone 30517)
文献数: 0
- 商品コード:MAB499-500
- メーカー:RSD
- 包装:500μg
- 価格:¥232,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | マッチドペア:Mouse IL-12/IL-23 p40 Allele-specific サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF499,スタンダードとして#499-ML-025を用いる。 クローン:30517 Genbank No: 16160 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse IL-12/IL-23 p40 MAb (Clone 30517)
文献数: 0
- 商品コード:MAB499-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | ※受注発注品。形状:溶液または凍結乾燥 クローン:30517 Genbank No: 16160 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | 法規備考 | ||||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | M1/M2 Macrophage Activation Marker Helper T Cells Marker Antibodies 使いっきり抗体 |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Mouse |
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Label | Unconjugated |
Immunogen | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑12 p40 |
Source | Monoclonal Rat IgG2B Clone # 30517 |
Purification | Protein A or G purified from hybridoma culture supernatant |
Specificity | Detects mouse IL-12 p40 in ELISAs. Polymorphisms exist in the mouse IL-12 p40 sequence. This antibody recognizes an allelic epitope found in the p40 subunit of several mouse strains including BALB/c, C57BL/6, CBA, C3H, DBA/1, NOR, I29/J, and A.SW. This antibody does not recognize the allelic variant that occurs in strains AKR, DBA/2, NOD, NZB, NZC, NZO, NZW, or SJL (1). In ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse (rm) IL-23, 30% cross-reactivity with rmIL-12 p70, and no cross-reactivity with recombinant human (rh) IL-12 p70 or rhIL-12 p40. |
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Applications and Data
Recommended Concentration | Sample | |
Mouse IL-12/IL-23 p40 Allele-specific Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse IL‑12/IL‑23 p40 Antibody (Catalog #MAB499 ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse IL‑12/IL‑23 p40 Biotinylated Antibody (Catalog #BAF499 ) |
ELISA Standard | Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein (Catalog #499-ML ) | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: IL-12/IL-23 p40 | Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 has multiple effects on T cells and NK cells and is a key mediator in the development of Th1 cells. IL-12 is a heterodimeric cytokine containing two disulfide-linked subunits, p35 and p40. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. Although mouse IL-12 is active on human or mouse IL-12 responsive cells, human IL-12 is not active on mouse cells. The disulfide-linked mouse p40 homodimer can bind to IL-12 receptors and is an antagonist of IL-12 activities in vitro. The mouse p40 monomer is at least ten times less active than the homodimer as an IL-12 antagonist. At the present time, the existence and the physiological role of mouse p40 homodimer in vivo remains to be determined. |
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References
Ymer, S. et al. (2002) Genes and Immunity 3:151. | |
Long Name: | Interleukin 12/Interleukin 23 p40 |
Entrez Gene IDs: | 3593 (Human); 16160 (Mouse); 64546 (Rat); 403976 (Canine); 493741 (Feline) |
Alternate Names: | CLMF2; IL12 p40; IL-12 p40; IL12B; IL-12B; NKSF2 |
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Related Research Areas
Cytokines and Receptors in Allergy and Asthma | ||
Cytokines and Receptors in Angiogenesis | ||
gamma delta T Cells | ||
IL-12 Family | ||
Th1 Cells | ||
Th17 Cells | ||
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Related Product & Information
Background | IL-12/IL-23 p40 |
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background_content | Background: IL-12/IL-23 p40 Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 has multiple effects on T cells and NK cells and is a key mediator in the development of Th1 cells.IL-12 is a heterodimeric cytokine containing two disulfide-linked subunits, p35 and p40. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. Although mouse IL-12 is active on human or mouse IL-12 responsive cells, human IL-12 is not active on mouse cells.The disulfide-linked mouse p40 homodimer can bind to IL-12 receptors and is an antagonist of IL-12 activities in vitro. The mouse p40 monomer is at least ten times less active than the homodimer as an IL-12 antagonist. At the present time, the existence and the physiological role of mouse p40 homodimer in vivo remains to be determined. |
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Citations
- IL-23 is required for neutrophil homeostasis in normal and neutrophilic mice.
Authors: Smith E, Zarbock A, Stark MA, Burcin TL, Bruce AC, Foley P, Ley K
J. Immunol., 2007;179(12):8274-9.
Species: Mouse
Sample Type: In Vivo
Application: In vivo - AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
Am. J. Pathol., 2002;160(4):1353-60.
Species: Mouse
Sample Type: Tissue Homogenates
Application: ELISA Development
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