抗Mouse IFN-gamma抗体(Anti-Mouse IFN-gamma antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IFN-gamma
Source	Monoclonal Rat IgG2A Clone # 37895
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IFN-gamma in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) IFN‑ gamma, rrIFN‑ gamma, rpIFN‑ gamma, rrmIFN‑ gamma, rfeIFN‑ gamma, or rcaIFN‑ gamma.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse IFN-gamma (Catalog # 485-MI)
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry	0.25 µg/106 cells	See below
Neutralization	Measured by its ability to neutralize IFN‑ gamma inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.075-0.3 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse IFN‑ gamma.

Intracellular Staining by Flow Cytometry
	Detection of IFN‑ gamma in Mouse Splenoctyes by Flow Cytometry. Mouse splenocyteseither (A) stimulated to induce Th1 cells or (B) unstimulated were stained with Rat Anti-Mouse IFN‑ gamma Monoclonal Antibody (Catalog # MAB485) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB554A). Quadrant markers were set based on control antibody staining (Catalog # MAB006). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Neutralization
	IFN‑ gamma Inhibition ofEMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma Antibody. Recombinant Mouse IFN‑ gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Mouse IFN‑ gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IFN‑ gamma Monoclonal Antibody (Catalog # MAB485). The ND50 is typically 0.075-0.3 µg/mL.

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Related Product & Information

Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Background	IFN-gamma
background_content	Background: IFN-gamma Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototypepro-inflammatory cytokine (1, 2). Mature mouse IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with rat IFN-gamma and 38-44% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus macaque IFN-gamma. IFN-gamma dimers bind to IFN‑ gamma  RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits anti-viral, anti-proliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

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Citations

1. Intraperitoneal infusion of mesenchymal stem cell attenuates severity of collagen antibody induced arthritis
   Authors: Y Nam, SM Jung, YA Rim, H Jung, K Lee, N Park, J Kim, Y Jang, YB Park, SH Park, JH Ju
   PLoS ONE, 2018;13(6):e0198740.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


2. Crucial role of OX40/OX40L signaling in a murine model of asthma
   Authors: W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang
   Mol Med Rep, 2018;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


3. Armc5 deletion causes developmental defects and compromises T-cell immune responses
   Authors: Y Hu, L Lao, J Mao, W Jin, H Luo, T Charpentie, S Qi, J Peng, B Hu, MM Marcinkiew, A Lamarre, J Wu
   Nat Commun, 2017;8(0):13834.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


4. Transcriptional regulator CTR9 inhibits Th17 differentiation via repression of IL-17 expression.
   Authors: Yoo, Hyeon-Se, Choi, Yongwook, Ahn, Narae, Lee, Saseong, Kim, Wan-Uk, Jang, Min Seon, Jang, Myoung H, Kim, Yon Su, Yoo, Joo-Yeon
   J Immunol, 2014;192(4):1440-8.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Functional Assay


5. CD4(+) T cells from food allergy model are resistant to TCR-dependent apoptotic induction.
   Authors: He W, Yang C, Xia L, Zhao M, Ge R, Huang H, Ji Q, Yang P, Liu Z
   Cytokine, 2014;68(1):32-9.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


6. Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
   Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
   FASEB J., 2011;25(1):409-19.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


7. The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function.
   Authors: Jin W, Qi S, Luo H
   BMC Immunol., 2011;12(0):68.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


8. IL-21 Promotes Skin Recruitment of CD4+ Cells and Drives IFN-{gamma}-Dependent Epidermal Hyperplasia.
   Authors: Sarra M, Caruso R, Cupi ML, Monteleone I, Stolfi C, Campione E, Diluvio L, Mazzotta A, Botti E, Chimenti S, Costanzo A, MacDonald TT, Pallone F, Monteleone G
   J. Immunol., 2011;186(9):5435-42.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


9. IL-13 from Th2-type cells suppresses induction of antigen-specific Th1 immunity in a T-cell lymphoma.
   Authors: Deepak P, Kumar S, Kishore D, Acharya A
   Int. Immunol., 2010;22(1):53-63.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


10. Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease.
    Authors: Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM
    J. Immunol., 2010;184(8):4159-69.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo

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