抗VEGF-D抗体(Anti-VEGF-D, Rat-Mono antibody)
掲載日情報：2021/01/28 現在Webページ番号：28392
VEGF-Dに対する抗体(Anti-VEGF-D, Rat-Mono )です。
※ 本製品は研究用です。研究用以外には使用できません。
追加しました。
- 価格
- Product Details
- Applications and Data
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 ：2024年05月18日 08時55分現在]
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Anti-VEGF-D, Rat-Mono(90409) |
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Anti-Mouse VEGF-D MAb (Clone 90409) |
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[在庫・価格 ：2024年05月18日 08時55分現在]
Anti-VEGF-D, Rat-Mono(90409)
文献数： 2
- 商品コード：MAB469
- メーカー：RSD
- 包装：500μg
- 価格：¥121,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
マッチドペア：Mouse VEGF-D サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF469，スタンダードとして#469-VD-025を用いる。 別名：c-fos induced growth factor (vascular endothelial growth factor D) クローン：90409 Genbank No： 2277 Protein Accession No： P97946 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | Mouse | 免疫動物 | Rat | ||||
| 交差性 | Mouse | 適用 | ELISA,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse VEGF-D MAb (Clone 90409)
文献数： 0
- 商品コード：MAB469-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥30,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
※受注発注品。形状：溶液または凍結乾燥 別名：c-fos induced growth factor (vascular endothelial growth factor D) クローン：90409 Genbank No： 2277 Protein Accession No： P97946 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Mouse | 適用 | ELISA,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | |||||||
| 関連記事 |
R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
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| Label | Unconjugated |
| Immunogen | E. coli-derived recombinant mouse VEGF-DPhe98-Ser206Accession # P97946 |
| Source | Monoclonal Rat IgG2A Clone # 90409 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects mouse VEGF-D in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) VEGF165, rmVEGF-B186, recombinant human (rh) VEGF-C, or rhVEGF-D is observed. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Mouse VEGF-D (Catalog # 469-VD) |
| Mouse VEGF-D Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse VEGF‑D Antibody (Catalog #MAB469 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse VEGF‑D Biotinylated Antibody (Catalog #BAF469 ) |
| ELISA Standard | Recombinant Mouse VEGF-D Protein (Catalog #469-VD ) | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | Preparation and Storage | |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: VEGF-D | Vascular endothelia growth factor D (VEGF-D), also known as c-fos-induced growth factor (FIGF), is a secreted glycoprotein of the VEGF/PDGF family. VEGFs regulate angiogenesis and lymphangiogenesis during development and tumor growth, and are characterized by eight conserved cysteine residues that form a cysteine-knot structure (1‑3). VEGF-C and VEGF-D, which share 23% amino acid (aa) sequence identity, are uniquely expressed as preproproteins that contain long N- and C-terminal propeptide extensions around the VEGF homology domain (VHD) (1, 2). Proteolytic processing of either 358 aa or 326 aa splice forms of mouse VEGF-D preproprotein creates a secreted proprotein. Further processing by extracellular serine proteases, such as plasmin or furin-like proprotein convertases, forms mature VEGF-D consisting of non-covalently linked 42 kDa homodimers of the 117 aa VHD (4‑7). Mature mouse VEGF-D shares 94%, 99%, 93%, 91% and 89% aa identity with the VHD of human, rat, equine, canine and bovine VEGF-D, respectively. It is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin (1 - 4). Mouse and human VEGF-D are ligands for VEGF receptor 3 (VEGF-R3, also called Flt-4) that are active across species and show enhanced affinity when processed (8). Unlike human VEGF-D, which is also a ligand for VEGF-R2 (also called Flk-1 or KDR), mouse VEGF-D does not bind to VEGF-R2 (8). VEGF-R3 is strongly expressed in lymphatic endothelial cells and is essential for regulation of the growth and differentiation of lymphatic endothelium (1, 2). While VEGF-C is the critical ligand for VEGF-R3 during embryonic lymphatic development, VEGF-D is most active in neonatal lymphatic maturation and bone growth (9‑11). Both promote tumor lymphangiogenesis (12). Consonant with their activity on VEGF receptors, binding of VEGF-C and VEGF-D to neuropilins contributes to VEGF-R3 signaling in lymphangiogenesis, while binding to integrin alpha 9 beta 1 mediates endothelial cell adhesion and migration (13, 14). |
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References
| Roy, H. et al. (2006) FEBS Lett. 580:2879. | |
| Otrock, Z.H. et al. (2007) Blood Cells Mol. Dis. 38:258. | |
| Orlandini, M. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11675. | |
| Stacker, S.A. et al. (1999) J. Biol. Chem. 274:32127. | |
| McColl, B.K. et al. (2003) J. Exp. Med. 198:863. | |
| McColl, B.K. et al. (2007) FASEB J. 21:1088. | |
| Baldwin, M.E. et al. (2001) J. Biol. Chem. 276:44307. | |
| Baldwin, M.E. et al. (2001) J. Biol. Chem. 276:19166. | |
| Baldwin, M.E. et al. (2005) Mol. Cell. Biol. 25:2441. | |
| Karpanen, T. et al. (2006) Am. J. Pathol. 169:708. | |
| Orlandini, M. et al. (2006) J. Biol. Chem. 281:17961. | |
| Stacker, S.A. et al. (2001) Nature Med. 7:186. | |
| Karpanen, T. et al. (2006) FASEB J. 20:1462. | |
| Vlahakis, N.E. et al. (2005) J. Biol. Chem. 280:4544. | |
| Long Name: | Vascular Endothelial Growth Factor D/cFos-induced Growth Factor |
| Entrez Gene IDs: | 2277 (Human); 14205 (Mouse) |
| Alternate Names: | c-fos induced growth factor (vascular endothelial growth factor D); FIGF; vascular endothelial growth factor D; VEGFD; VEGF-D; VEGF-DVEGFDc-Fos-induced growth factor |
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Related Research Areas
| VEGF Family | ||
| VEGF Family Ligands | ||
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Related Product & Information
| Background | VEGF-D |
|---|---|
| background_content | Background: VEGF-D Vascular endothelia growth factor D (VEGF-D), also known as c-fos-induced growth factor (FIGF), is a secreted glycoprotein of the VEGF/PDGF family. VEGFs regulate angiogenesis and lymphangiogenesis during development and tumor growth, and are characterized by eight conserved cysteine residues that form a cysteine-knot structure (1‑3). VEGF-C and VEGF-D, which share 23% amino acid (aa) sequence identity, are uniquely expressed as preproproteins that contain long N- and C-terminal propeptide extensions around the VEGF homology domain (VHD) (1, 2). Proteolytic processing of either 358 aa or 326 aa splice forms of mouse VEGF-D preproprotein creates a secreted proprotein. Further processing by extracellular serine proteases, such as plasmin or furin-like proprotein convertases, forms mature VEGF-D consisting of non-covalently linked 42 kDa homodimers of the 117 aa VHD (4‑7). Mature mouse VEGF-D shares 94%, 99%, 93%, 91% and 89% aa identity with the VHD of human, rat, equine, canine and bovine VEGF-D, respectively. It is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin (1 - 4). Mouse and human VEGF-D are ligands for VEGF receptor 3 (VEGF-R3, also called Flt-4) that are active across species and show enhanced affinity when processed (8). Unlike human VEGF-D, which is also a ligand for VEGF-R2 (also called Flk-1 or KDR), mouse VEGF-D does not bind to VEGF-R2 (8). VEGF-R3 is strongly expressed in lymphatic endothelial cells and is essential for regulation of the growth and differentiation of lymphatic endothelium (1, 2). While VEGF-C is the critical ligand for VEGF-R3 during embryonic lymphatic development, VEGF-D is most active in neonatal lymphatic maturation and bone growth (9‑11). Both promote tumor lymphangiogenesis (12). Consonant with their activity on VEGF receptors, binding of VEGF-C and VEGF-D to neuropilins contributes to VEGF-R3 signaling in lymphangiogenesis, while binding to integrin alpha 9 beta 1 mediates endothelial cell adhesion and migration (13, 14). |
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Citations
- VEGF-D-induced draining lymphatic enlargement and tumor lymphangiogenesis promote lymph node metastasis in a xenograft model of ovarian carcinoma.
Authors: Du, Li-Cheng, Chen, Xian-Che, Wang, Dong, Wen, Yan-Jun, Wang, Chun-Tin, Wang, Xue-Mei, Kan, Bing, Wei, Yu-Quan, Zhao, Xia
Reprod Biol Endocrinol, 2014;12(0):14.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded - A tumor-selective biotherapy with prolonged impact on established metastases based on cytokine gene-engineered MSCs.
Authors: Chen X, Lin X, Zhao J, Shi W, Zhang H, Wang Y, Kan B, DU L, Wang B, Wei Y, Liu Y, Zhao X
Mol. Ther., 2008;16(4):749-56.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded
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