抗Mouse VEGF R2/Flk-1抗体(Anti-Mouse VEGF R2/Flk-1 antibody)
掲載日情報:2021/01/28 現在Webページ番号:28363
Mouse VEGF R2/Flk-1に対する抗体(Anti-Mouse VEGF R2/Flk-1 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Related Product & Information
- Citations
価格
[在庫・価格 :2025年04月26日 20時35分現在]
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Anti-Mouse VEGF R2/Flk-1 MAb (Clone 91202) |
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Anti-Mouse VEGF R2/Flk-1 MAb (Clone 91202) |
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Anti-Mouse VEGF R2/Flk-1 MAb (Clone 91202) |
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[在庫・価格 :2025年04月26日 20時35分現在]
Anti-Mouse VEGF R2/Flk-1 MAb (Clone 91202)
文献数: 1
- 商品コード:MAB4431-100
- メーカー:RSD
- 包装:100μg
- 価格:¥92,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | 別名:CD309 クローン:91202 Genbank No: 3791 Protein Accession No: P35918 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Neutralising | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | |||||||
関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse VEGF R2/Flk-1 MAb (Clone 91202)
文献数: 1
- 商品コード:MAB4431-500
- メーカー:RSD
- 包装:500μg
- 価格:¥232,000
- 在庫:無(未発注)
- 納期:10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | マッチドペア:Mouse VEGF R2/Flk-1 サンドイッチELISAの補足用抗体として利用可能,検出用抗体として#BAF644,スタンダードとして#443-KD-050を用いる。 別名:CD309 クローン:91202 Genbank No: 3791 Protein Accession No: P35918 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Neutralising | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | Myeloid-derived Suppressor Cell Marker |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
Anti-Mouse VEGF R2/Flk-1 MAb (Clone 91202)
文献数: 1
- 商品コード:MAB4431-SP
- メーカー:RSD
- 包装:25μg
- 価格:¥30,000
- 在庫:無(未発注)
- 納期:2~3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等:
説明文 | ※受注発注品。形状:溶液または凍結乾燥 別名:CD309 クローン:91202 Genbank No: 3791 Protein Accession No: P35918 |
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別包装品 | 別包装品あり | ||||||
法規制等 | |||||||
保存条件 | -20℃ | 法規備考 | |||||
抗原種 | 免疫動物 | Rat | |||||
交差性 | Mouse | 適用 | ELISA,Neutralising | ||||
標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
吸収処理 | クラス | IgG | |||||
クロナリティ | Monoclonal | フォーマット | |||||
掲載カタログ |
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製品記事 | 使いっきり抗体 |
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関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
Species Reactivity | Mouse |
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Label | Unconjugated |
Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse VEGF R2Ala20-Glu762Accession # P35918 |
Source | Monoclonal Rat IgG2A Clone # 91202 |
Purification | Protein A or G purified from hybridoma culture supernatant |
Specificity | Detects mouse VEGF R2 in Western blots. |
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Applications and Data
Recommended Concentration | Sample | |
Mouse VEGF R2/Flk-1 Sandwich Immunoassay | Reagent | |
ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse VEGF R2/KDR/Flk‑1 Antibody (Catalog #MAB4431 ) |
ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse VEGF R2/KDR/Flk‑1 Biotinylated Antibody (Catalog #BAF644 ) |
ELISA Standard | Recombinant Mouse VEGF R2/Flk-1 Fc Chimera Protein (Catalog #443-KD ) | |
Neutralization | Measured by its ability to neutralize VEGF R2/KDR/Flk‑1-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 0.05-0.15 µg/mL in the presence of 150 ng/mL Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera and 5 ng/mL Recombinant Mouse VEGF164. | |
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. |
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Data Examples
Neutralization |
![]() click image to view larger | VEGF R2/KDR/Flk‑1 Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Mouse VEGF R2/KDR/Flk‑1 Antibody.Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera (Catalog # 443-KD) inhibits Recombinant Mouse VEGF164 (Catalog # 493-MV) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse VEGF164 (5 ng/mL) activity elicited by Recombinant Mouse VEGF R2/KDR/Flk‑1 Fc Chimera (150 ng/mL) is neutralized (green line) by increasing concentrations of Mouse VEGF R2/KDR/Flk‑1 Monoclonal Antibody (Catalog # MAB4431). The ND50 is typically 0.05-0.15 µg/mL. |
Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
Background: VEGF R2/KDR/Flk-1 | VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mouse VEGF R2 cDNA encodes a 1367 amino acid (aa) precursor protein with a 19 aa signal peptide. Mature VEGF R2 is composed of a 743 aa extracellular domain, a 22 aa transmembrane domain, and a 583 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist. |
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References
Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4. | |
Achen, M.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548. | |
Long Name: | Vascular Endothelial Growth Factor Receptor 2 |
Entrez Gene IDs: | 3791 (Human); 16542 (Mouse) |
Alternate Names: | CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2 |
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Related Research Areas
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Related Product & Information
Long Name | Vascular Endothelial Growth Factor Receptor 2 |
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Entrez Gene IDs | 3791 (Human); 16542 (Mouse) |
Background | VEGF R2/KDR/Flk-1 |
background_content | Background: VEGF R2/KDR/Flk-1 VEGF R2 (KDR/Flk-1), VEGF R1 (Flt-1) and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGF R1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mouse VEGF R2 cDNA encodes a 1367 amino acid (aa) precursor protein with a 19 aa signal peptide. Mature VEGF R2 is composed of a 743 aa extracellular domain, a 22 aa transmembrane domain, and a 583 aa cytoplasmic domain. In contrast to VEGF R1 which binds both PlGF and VEGF with high affinity, VEGF R2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGF R2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist. |
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Citations
- Mycobacterium tuberculosis exploits the formation of new blood vessels for its dissemination
Sci Rep, 2016;6(0):33162.
Species: Mouse
Sample Type: In Vivo
Application: In vivo - VEGF-A modulates expression of inhibitory checkpoints on CD8+ T cells in tumors.
Authors: Voron T, Colussi O, Marcheteau E, Pernot S, Nizard M, Pointet A, Latreche S, Bergaya S, Benhamouda N, Tanchot C, Stockmann C, Combe P, Berger A, Zinzindohoue F, Yagita H, Tartour E, Taieb J, Terme M
J Exp Med, 2015;212(2):139-48.
Species: Mouse
Sample Type: Whole Cells
Application: Neut - Spontaneous CNV in a novel mutant mouse is associated with early VEGF-A-driven angiogenesis and late-stage focal edema, neural cell loss, and dysfunction.
Authors: Nagai, Norihiro, Lundh von Leithner, Pete, Izumi-Nagai, Kanako, Hosking, Brett, Chang, Bo, Hurd, Ron, Adamson, Peter, Adamis, Anthony, Foxton, Richard, Ng, Yin Shan, Shima, David T
Invest Ophthalmol Vis Sci, 2014;55(6):3709-19.
Species: Mouse
Sample Type: In Vivo
Application: Neut - alpha2beta1 integrin expression in the tumor microenvironment enhances tumor angiogenesis in a tumor cell-specific manner.
Authors: Zhang Z, Ramirez NE, Yankeelov TE, Li Z, Ford LE, Qi Y, Pozzi A, Zutter MM
Blood, 2007;111(4):1980-8.
Species: Mouse
Sample Type: Cell Lysates
Application: WB
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