抗Mouse GM-CSF抗体(Anti-Mouse GM-CSF antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	Yeast derived recombinant mouse GM-CSFAccession # P01587
Source	Monoclonal Rat IgG2A Clone # MP122E9
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse GM-CSF in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human GM‑CSF.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse GM-CSF (Catalog # 415-ML) under non-reducing conditions only
Mouse GM-CSF Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse GM‑CSF Antibody (Catalog #MAB415 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse GM‑CSF Biotinylated Antibody (Catalog #BAF415 )
ELISA Standard		Recombinant Mouse GM-CSF Protein (Catalog #415-ML )
Neutralization	Measured by its ability to neutralize GM‑CSF-induced proliferation in the DA3 mouse myeloma cell line. Ihle, J.N. et al. (1984) Advances in Viral Oncology. In G. Klein (eds): Raven Press, New York, NY. 4:95. The Neutralization Dose (ND50) is typically 0.03-0.09 µg/mL in the presence of 0.75 ng/mL Recombinant Mouse GM‑CSF.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody.Recombinant Mouse GM‑CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415). The ND50 is typically 0.03-0.09 µg/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GM-CSF	GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices (8-10). Mature mouse GM-CSF shares 49-54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GM-CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (13). The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells (14, 15).

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References

Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
Cao, Y. (2007) J. Clin. Invest. 117:2362.
Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
Heuser, M. et al. (2007) Semin. Hematol. 44:148.
Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
Kaushansky, K. et al. (1992) Biochemistry 31:1881.
Diederichs, K. et al. (1991) Science 254:1779.
Gough, N.M. et al. (1984) Nature 309:763.
Onetto-Pothier, N. et al. (1990) Blood 75:59.
Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9655.
Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
Oaks, M.K. et al. (1995) J. Interferon Cytokine Res. 15:1095.
Vandenabeele, P. et al. (1990) Lymphokine Res. 9:381.
Long Name:	Granulocyte Macrophage Growth Factor
Entrez Gene IDs:	1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
Alternate Names:	colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; molgramostin; sargramostim

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Related Research Areas

Common beta Chain Receptor Family

Cytokines and Receptors in Allergy and Asthma

Cytokines and Receptors in Angiogenesis

Dendritic Cell Development

Gametes and Fertilization

gamma delta T Cells

Inflammatory Mediators

Isolation, Culture, and Identification of Natural Killer Cells

Macrophage Activation Markers

Myeloid-derived Suppressor Cells (MDSC)

Natural Killer T (NKT) Cells

T Cell Cytokine Signaling

Targeting GM-CSF in epithelial and vascular remodeling in experimental Eosinophilic Esophagitis Authors: Eóin N McNamee Allergy, 2016;0(0):. Species: Mouse Sample Type: In Vivo Application: In Vivo

Neutralization

Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF Antibody. Recombinant Mouse GM‑CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM‑CSF (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse GM‑CSF Monoclonal Antibody (Catalog # MAB415). The ND50 is typically 0.03-0.09 µg/mL.

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Related Product & Information

Long Name	Granulocyte Macrophage Growth Factor
Entrez Gene IDs	1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
Background	GM-CSF
background_content	Background: GM-CSF GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3-5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices (8-10). Mature mouse GM-CSF shares 49-54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GM-CSF. GM‑CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (11, 12). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (13). The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells (14, 15).

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Citations

1. Targeting GM-CSF in epithelial and vascular remodeling in experimental Eosinophilic Esophagitis
   Authors: Eóin N McNamee
   Allergy, 2016;0(0):.
   Species: Mouse
   Sample Type: In Vivo
   Application: In Vivo


2. Type I interferons and microbial metabolites of tryptophan modulate astrocyte activity and central nervous system inflammation via the aryl hydrocarbon receptor
   Nat Med, 2016;0(0):.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


3. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
   Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
   J Biol Chem, 2015;290(37):22338-51.
   Species: Mouse
   Sample Type: Whole Cells
   Application: Neut


4. Thioredoxin-1 protects against neutrophilic inflammation and emphysema progression in a mouse model of chronic obstructive pulmonary disease exacerbation.
   Authors: Tanabe, Naoya, Hoshino, Yuma, Marumo, Satoshi, Kiyokawa, Hirofumi, Sato, Susumu, Kinose, Daisuke, Uno, Kazuko, Muro, Shigeo, Hirai, Toyohiro, Yodoi, Junji, Mishima, Michiaki
   PLoS ONE, 2013;8(11):e79016.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


5. Properties of bcr-abl-transformed mouse 12B1 cells secreting interleukin-2 and granulocyte-macrophage colony stimulating factor (GM-CSF): II. Adverse effects of GM-CSF.
   Authors: Petrackova M, Stanek L, Mandys V, Dundr P, Vonka V
   Int. J. Oncol., 2012;40(6):1915-22.
   Species: Mouse
   Sample Type: In Vivo
   Application: In Vivo


6. Response patterns of cytokines/chemokines in two murine strains after irradiation.
   Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
   Cytokine, 2012;58(2):169-77.
   Species: Mouse
   Sample Type: Plasma
   Application: Luminex Assay Development


7. Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice.
   Authors: Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH
   J. Clin. Invest., 2011;121(11):4446-61.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


8. The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation.
   Authors: Wengner AM, Pitchford SC, Furze RC, Rankin SM
   Blood, 2007;111(1):42-9.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


9. Recombinant Leishmania major secreting biologically active granulocyte-macrophage colony-stimulating factor survives poorly in macrophages in vitro and delays disease development in mice.
   Authors: Dumas C, Muyombwe A, Roy G, Matte C, Ouellette M, Olivier M, Papadopoulou B
   Infect. Immun., 2003;71(11):6499-509.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ICC


10. Tick salivary gland extract-activated transmission of Borrelia afzelii spirochaetes.
    Authors: Pechova J, Stĕpánová G, Kovar L, Kopecky J
    Folia Parasitol., 2002;49(2):153-9.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development

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