抗Mouse IL-13抗体(Anti-Mouse IL-13 antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Mouse
Label	Unconjugated
Immunogen	E. coli-derived recombinant mouse IL-13Accession # P20109
Source	Monoclonal Rat IgG2A Clone # 38213
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects mouse IL-13 in ELISAs and Western blots. In ELISAs, less than 0.1% cross-reactivity with recombinant human IL‑13 is observed.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Mouse IL‑13 (Catalog # 413-ML)
Mouse IL-13 Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Mouse IL‑13 Antibody (Catalog #MAB413 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Mouse IL‑13 Biotinylated Antibody (Catalog #BAF413 )
ELISA Standard		Recombinant Mouse IL-13 Protein (Catalog #413-ML )
Neutralization	Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.3-0.8 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑13.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Neutralization	click image to view larger	Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody.Recombinant Mouse IL‑13 (Catalog # 413-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑13 Monoclonal Antibody (Catalog # MAB413). The ND50 is typically 0.3-0.8 µg/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-13	IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of pro-inflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

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References

Wills-Karp, M. (2004) Immunol. Rev. 202:175.
Nakajima, H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
Brown, K.D. et al. (1989) J. Immunol. 142:679.
Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387.
Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun. 360:464.
Tabata, Y. et al. (2007) J. Immunol. 177:7905.
Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
Andrews, A-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.
Long Name:	Interleukin 13
Entrez Gene IDs:	3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
Alternate Names:	ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600

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Related Research Areas

Show

Cytokines and Receptors in Allergy and Asthma
Cytokines and Receptors in Angiogenesis
Cytokines and Receptors in Tfh Cell Differentiation and Function
gamma delta T Cells
Group 2 ILCs
Myeloid-derived Suppressor Cells (MDSC)
Natural Killer T (NKT) Cells
T Cell Cytokine Signaling
Th2 Cells
Th22 Cells
Sphingosine-1-phosphate receptor-2 facilitates pulmonary fibrosis through potentiating IL-13 pathway in macrophages Authors: J Zhao, Y Okamoto, Y Asano, K Ishimaru, S Aki, K Yoshioka, N Takuwa, T Wada, Y Inagaki, C Takahashi, T Nishiuchi, Y Takuwa PLoS ONE, 2018;13(5):e0197604. Species: Mouse Sample Type: Application: In vivo
Images	Ratings	Applications	Species	Reviewed By	Date	Details
	Excellent		ELISA	Mouse	Anonymous	04/01/2018

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Summary

Application	ELISA
Sample Tested	Tumor cell lyastes
Species	Mouse

Neutralization

Cell Proliferation Induced by IL‑13 and Neutralization by Mouse IL‑13 Antibody. Recombinant Mouse IL‑13 (Catalog # 413-ML) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑13 Monoclonal Antibody (Catalog # MAB413). The ND50 is typically 0.3-0.8 µg/mL.

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Related Product & Information

Long Name	Interleukin 13
Entrez Gene IDs	3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)
Background	IL-13
background_content	Background: IL-13 IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1-3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature mouse IL-13 shares 57%, 75%, and 58% amino acid sequence identity with human, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of pro-inflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11-14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).

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Citations

1. Sphingosine-1-phosphate receptor-2 facilitates pulmonary fibrosis through potentiating IL-13 pathway in macrophages
   Authors: J Zhao, Y Okamoto, Y Asano, K Ishimaru, S Aki, K Yoshioka, N Takuwa, T Wada, Y Inagaki, C Takahashi, T Nishiuchi, Y Takuwa
   PLoS ONE, 2018;13(5):e0197604.
   Species: Mouse
   Sample Type:
   Application: In vivo


2. Macrophage migration inhibitory factor deficiency enhances immune response to Nippostrongylus brasiliensis
   Authors: SR Damle, RK Martin, JV Cross, DH Conrad
   Mucosal Immunol, 2016;0(0):.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


3. RSV vaccine-enhanced disease is orchestrated by the combined actions of distinct CD4 T cell subsets.
   Authors: Knudson C, Hartwig S, Meyerholz D, Varga S
   PLoS Pathog, 2015;11(3):e1004757.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Capture


4. CD8(+) cells regulate the T helper-17 response in an experimental murine model of Sjogren syndrome.
   Authors: Zhang X, Schaumburg C, Coursey T, Siemasko K, Volpe E, Gandhi N, Li D, Niederkorn J, Stern M, Pflugfelder S, de Paiva C
   Mucosal Immunol, 2014;7(2):417-27.
   Species: Mouse
   Sample Type: Whole Cells
   Application: ELISA Capture


5. Chlamydial respiratory infection during allergen sensitization drives neutrophilic allergic airways disease.
   Authors: Horvat JC, Starkey MR, Kim RY, Beagley KW, Preston JA, Gibson PG, Foster PS, Hansbro PM
   J. Immunol., 2010;184(8):4159-69.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


6. Breast milk immune complexes are potent inducers of oral tolerance in neonates and prevent asthma development.
   Authors: Mosconi E, Rekima A, Seitz-Polski B
   Mucosal Immunol, 2010;3(5):461-74.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


7. IL-6 is required for airway mucus production induced by inhaled fungal allergens.
   Authors: Neveu WA, Allard JB, Dienz O, Wargo MJ, Ciliberto G, Whittaker LA, Rincon M
   J. Immunol., 2009;183(3):1732-8.
   Species: Mouse
   Sample Type: Cell Culture Supernates
   Application: ELISA Development


8. FTY720 ameliorates oxazolone colitis in mice by directly affecting T helper type 2 functions.
   Authors: Daniel C, Sartory NA, Zahn N
   Mol. Immunol., 2007;44(13):3305-16.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


9. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
   Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
   Am. J. Pathol., 2006;168(6):1940-50.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: ELISA Development


10. Loss of pro-apoptotic Bim promotes accumulation of pulmonary T lymphocytes and enhances allergen-induced goblet cell metaplasia.
    Authors: Pierce J, Rir-Sima-Ah J, Estrada I, Wilder J, Strasser A, Tesfaigzi Y
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(5):L862-70.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ELISA Development

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