抗Mouse IL-3抗体(Anti-Mouse IL-3 antibody)
掲載日情報：2021/01/28 現在Webページ番号：28342
Mouse IL-3に対する抗体(Anti-Mouse IL-3 )です。
※ 本製品は研究用です。研究用以外には使用できません。
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- 価格
- Product Details
- Applications and Data
- Data Examples
- References
- Related Research Areas
- Summary
- Summary
- Related Product & Information
- Citations
価格
[在庫・価格 ：2025年07月22日 14時35分現在]
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Anti-Mouse IL-3 MAb (Clone MP28F8) |
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[在庫・価格 ：2025年07月22日 14時35分現在]
Anti-Mouse IL-3 MAb (Clone MP28F8)
文献数： 0
- 商品コード：MAB403-100
- メーカー：RSD
- 包装：100μg
- 価格：¥83,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別名：Hematopoietic growth factor クローン：MP28F8 Genbank No： 3562 Protein Accession No： P01586 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Mouse | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse IL-3 MAb (Clone MP28F8)
文献数： 0
- 商品コード：MAB403-500
- メーカー：RSD
- 包装：500μg
- 価格：¥276,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | マッチドペア：Mouse IL-3 サンドイッチELISAの補足用抗体として利用可能，検出用抗体として#BAF403，スタンダードとして#403-ML-010を用いる。 別名：Hematopoietic growth factor クローン：MP28F8 Genbank No： 3562 Protein Accession No： P01586 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | -20℃ | 法規備考 | |||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Mouse | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
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| 製品記事 | 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Anti-Mouse IL-3 MAb (Clone MP28F8)
文献数： 0
- 商品コード：MAB403-SP
- メーカー：RSD
- 包装：25μg
- 価格：¥25,000
- 在庫：無(未発注)
- 納期：2～3週間 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ※受注発注品。形状：溶液または凍結乾燥 別名：Hematopoietic growth factor クローン：MP28F8 Genbank No： 3562 Protein Accession No： P01586 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | |||||||
| 保存条件 | 法規備考 | ||||||
| 抗原種 | 免疫動物 | Rat | |||||
| 交差性 | Mouse | 適用 | ELISA,Neutralising,Western Blot | ||||
| 標識 | Unlabeled | 性状 | Protein A/G Affinity Purified | ||||
| 吸収処理 | クラス | IgG | |||||
| クロナリティ | Monoclonal | フォーマット | |||||
| 掲載カタログ |
|
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| 製品記事 | 抗幹細胞マーカー抗体／抗造血幹細胞マーカー抗体（R&D Systems社） 使いっきり抗体 |
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| 関連記事 | R&D Systems(R&Dシステムズ)社 ELISA用ペア抗体を使用したELISA 構築ガイド |
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Product Details
| Species Reactivity | Mouse |
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| Label | Unconjugated |
| Immunogen | COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse IL-3Accession # P01586 |
| Source | Monoclonal Rat IgG1 Clone # MP28F8 |
| Purification | Protein A or G purified from hybridoma culture supernatant |
| Specificity | Detects mouse IL-3 in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant human (rh) IL‑3 or recombinant rat IL-3 is observed. Does not neutralize the biological activity of rhIL-3. |
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Applications and Data
| Recommended Concentration | Sample | |
| Western Blot | 1 µg/mL | Recombinant Mouse IL‑3 (Catalog # 403-ML) |
| Mouse IL-3 Sandwich Immunoassay | Reagent | |
| ELISA Capture (Matched Antibody Pair) | 2-8 µg/mL | Mouse IL‑3 Antibody (Catalog #MAB403 ) |
| ELISA Detection (Matched Antibody Pair) | 0.1-0.4 µg/mL | Mouse IL‑3 Biotinylated Antibody (Catalog #BAF403 ) |
| ELISA Standard | Recombinant Mouse IL-3 Protein (Catalog #403-ML ) | |
| Neutralization | Measured by its ability to neutralize IL‑3-induced proliferation in the NFS60 mouse myeloid cell line. The Neutralization Dose (ND50) is typically 0.05 - 0.15 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑3. | |
| Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website. | ||
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Data Examples
| Neutralization |
 click image to view larger | Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 Antibody.Recombinant Mouse IL‑3 (Catalog # 403-ML) stimulates proliferation in the NFS60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑3 Monoclonal Antibody (Catalog # MAB403). The ND50 is typically 0.05-0.15 µg/mL. |
| Reconstitution | Reconstitute at 0.5 mg/mL in sterile PBS. | Reconstitution Buffer Available |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C | |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. | |
| 1 month, 2 to 8 °C under sterile conditions after reconstitution. | ||
| 6 months, -20 to -70 °C under sterile conditions after reconstitution. | ||
| Background: IL-3 | Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated mouse mast cells, mouse keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and mouse IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on mouse cells. IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of alpha and beta subunits. The IL-3 R alpha is a member of the cytokine receptor super family and binds IL-3 with low affinity. Two distinct beta subunits, AIC2A ( beta IL-3) and AIC2B ( beta c) are present in mouse cells. beta IL-3 also binds IL-3 with low affinity and forms a high affinity receptor with the alpha subunit. The beta c subunits does not bind any cytokine but forms functional high affinity receptors with the alpha subunit of the IL-3, IL-5 and GM-CSF receptors. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells. |
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References
| Yokota, T. et al. (1984) Proc. Natl. Acad. Sci. USA 81:1070. | |
| Fung, M.C. et al. (1984) Nature 307:233. | |
| Miyatake, S. et al. (1985) Proc. Natl. Acad. Sci. USA 82:316. | |
| Long Name: | Interleukin 3 |
| Entrez Gene IDs: | 3562 (Human); 16187 (Mouse); 24495 (Rat) |
| Alternate Names: | Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor |
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Related Research Areas
| Common beta Chain Receptor Family | ||||||||
| Cytokines and Receptors in Allergy and Asthma | ||||||||
| Megakaryocytes | ||||||||
| T Cell Cytokine Signaling | ||||||||
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| Images | Ratings | Applications | Species | Reviewed By | Date | Details | ||
|---|---|---|---|---|---|---|---|---|
| Very Good | Functional Assay | Mouse | Anonymous | 07/27/2016 | ||||
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Summary
| Application | Functional Assay | |||||||
| Sample Tested | Splenocytes | |||||||
| Species | Mouse | |||||||
| Very Good | Flow | Mouse | Anonymous | 07/27/2016 |
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Summary
| Application | Flow Cytometry |
| Sample Tested | T cells |
| Species | Mouse |
| Neutralization | |
|---|---|
 | Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 Antibody. Recombinant Mouse IL‑3 (Catalog # 403-ML) stimulates proliferation in the NFS60 mouse myeloid cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL‑3 Monoclonal Antibody (Catalog # MAB403). The ND50 is typically 0.05-0.15 µg/mL. |
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Related Product & Information
| Long Name | Interleukin 3 |
|---|---|
| Entrez Gene IDs | 3562 (Human); 16187 (Mouse); 24495 (Rat) |
| Background | IL-3 |
| background_content | Background: IL-3 Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages. Because of its multiple functions and targets, it was originally studied under different names, including mast cell growth factor P-cell stimulating factor, burst promoting activity, multi-colony stimulating factor, thy-1 inducing factor and WEHI-3 growth factor. In addition to activated T cells, other cell types such as human thymic epithelial cells, activated mouse mast cells, mouse keratinocytes and neurons/astrocytes can also produce IL-3. At the amino acid sequence level, mature human and mouse IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on mouse cells.IL-3 exerts its biological activities through binding to specific cell surface receptors. The high affinity receptor responsible for IL-3 signaling is composed of alpha and beta subunits. The IL-3 R alpha is a member of the cytokine receptor super family and binds IL-3 with low affinity. Two distinct beta subunits, AIC2A ( beta IL-3) and AIC2B ( beta c) are present in mouse cells. beta IL-3 also binds IL-3 with low affinity and forms a high affinity receptor with the alpha subunit. The beta c subunits does not bind any cytokine but forms functional high affinity receptors with the alpha subunit of the IL-3, IL-5 and GM-CSF receptors. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils and various myeloid leukemic cells. |
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Citations
- The spleen is the site where mast cells are induced in the development of food allergy
Authors: S Toyoshima, E Wakamatsu, Y Ishida, Y Obata, Y Kurashima, H Kiyono, R Abe
Int. Immunol, 2017;0(0):.
Species: Mouse
Sample Type: Whole Cells
Application: Neut - All Trans Retinoic Acid, Transforming Growth Factor ? and Prostaglandin E2 in Mouse Plasma Synergize with Basophil-Secreted Interleukin-4 to M2 Polarize Murine Macrophages
PLoS ONE, 2016;11(12):e0168072.
Species: Mouse
Sample Type: Whole Cells
Application: Neut - Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
J Biol Chem, 2015;290(37):22338-51.
Species: Mouse
Sample Type: Whole Cells
Application: Neut - Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012;58(2):169-77.
Species: Mouse
Sample Type: Plasma
Application: Luminex Assay Development - Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen.
Authors: Tomaki M, Zhao LL, Sjostrand M, Linden A, Ichinose M, Lotvall J
Pulm Pharmacol Ther, 2002;15(2):161-8.
Species: Mouse
Sample Type:
Application: In vivo
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