抗TGF-beta 3抗体(Anti-TGF-beta 3 antibody)

• 価格
• Product Details
• Applications and Data
• Related Product & Information
• Citations

価格

目次に戻る

Product Details

Label	Unconjugated
Immunogen	Spodoptera frugiperda, Sf 21 (baculovirus) derived recombinant human TGF-beta 3Ala301-Ser412 (Tyr340Phe)Accession # P10600
Source	Monoclonal Mouse IgG1 Clone # 20724
Purification	Protein A or G purified from ascites
Specificity	Detects TGF-beta 3 from multiple species in direct ELISAs and Western blots. In Western blots, less than 25% cross-reactivity with recombinant human (rh) TGF‑ beta 1.2 and rhTGF-beta 2 is observed, and less than 2% cross‑reactivity with recombinant amphibian TGF-beta 5 and recombinant human TGF-beta 1 is observed. Neutralizes the biological activity of TGF-beta 3 but not TGF-beta 1, TGF-beta 2, or TGF-beta 5.

目次に戻る

Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	Recombinant Human TGF-beta 3 (Catalog # 243-B3) under non-reducing conditions only
Immunohistochemistry	8-25 µg/mL	See below
Neutralization	Measured by its ability to neutralize TGF‑ beta 3 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 0.1 ng/mL Recombinant Human TGF‑ beta 3 and 7.5 ng/mL Recombinant Mouse IL‑4.

Neutralization
	TGF‑ beta 3 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 3 Antibody. Recombinant Human TGF‑ beta 3 (Catalog # 243-B3) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 3 (0.1 ng/mL) is neutralized (green line) by increasing concen­trations of Mouse Anti-TGF‑ beta 3 Monoclonal Antibody (Catalog # MAB243). The ND50 is typically 0.1-0.3 µg/mL.
Immunohistochemistry
	TGF‑ beta 3 in Human Breast Cancer Tissue. TGF‑ beta 3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-TGF‑ beta 3 Monoclonal Antibody (Catalog # MAB243) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

目次に戻る

Related Product & Information

Long Name	Transforming Growth Factor beta 3
Entrez Gene IDs	7043 (Human); 21809 (Mouse); 25717 (Rat)
Background	TGF-beta 3
background_content	Background: TGF-beta 3 TGF-beta 3 (transforming growth factor beta 3) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure (1‑7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non-redundant functions; for TGF-beta 3, mice with targeted deletion show defects palatogenesis and pulmonary development (2). Human TGF-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 220 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF‑ beta 3 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 3 remain non-covalently associated after secretion, forming the small latent TGF-beta 3 complex (8‑10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 3 shows 100%, 99% and 98% aa identity with mouse/dog/horse, rat and pig TGF-beta 3, respectively. It demonstrates cross-species activity.(1) TGF-beta 3 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta  RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1.This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF-beta  RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts (11).

目次に戻る

Citations

1. TGF-beta3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses.
   Authors: Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, Komai T, Shoda H, Miyazaki J, Fujio K, Yamamoto K
   Nat Commun, 2015;6(0):6329.
   Species: Mouse
   Sample Type: In Vivo
   Application: In vivo


2. Expression profiling of nuclear receptors identifies key roles of NR4A subfamily in uterine fibroids.
   Authors: Yin H, Lo J, Kim J, Marsh E, Kim J, Ghosh A, Bulun S, Chakravarti D
   Mol Endocrinol, 2013;27(5):726-40.
   Species: Human
   Sample Type: Tissue Homogenates
   Application: WB


3. Systematic analysis of palatal transcriptome to identify cleft palate genes within TGFbeta3-knockout mice alleles: RNA-Seq analysis of TGFbeta3 Mice.
   Authors: Ozturk F, Li Y, Zhu X, Guda C, Nawshad A
   BMC Genomics, 2013;14(0):113.
   Species: Mouse
   Sample Type: Tissue Homogenates
   Application: WB


4. An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition.
   Authors: Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, Morris M, Wyatt L, Farshid G, Lim YY, Lindeman GJ, Shannon MF, Drew PA, Khew-Goodall Y, Goodall GJ
   Mol. Biol. Cell, 2011;22(10):1686-98.
   Species: Canine
   Sample Type: Whole Cells
   Application: Neut


5. Differential versican isoforms and aggrecan expression in the chicken embryo aorta.
   Authors: Arciniegas E, Neves CY, Candelle D, Parada D
   Anat Rec A Discov Mol Cell Evol Biol, 2004;279(1):592-600.
   Species: Chicken
   Sample Type: Whole Tissue
   Application: IHC


6. Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells.
   Authors: Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI
   Am. J. Physiol. Lung Cell Mol. Physiol., 2002;282(5):L1049-56.
   Species: Human
   Sample Type: Cell Culture Supernates
   Application: ELISA Development

目次に戻る