抗Human IL-6 R alpha抗体(Anti-Human IL-6 R alpha antibody)

• 価格
• Product Details
• Applications and Data
• Data Examples
• References
• Related Research Areas
• Summary
• Summary
• Related Product & Information
• Citations

価格

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Product Details

Species Reactivity	Human
Label	Unconjugated
Immunogen	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑6 RLeu20-Asp339Accession # P08887
Source	Monoclonal Mouse IgG1 Clone # 17506
Purification	Protein A or G purified from hybridoma culture supernatant
Specificity	Detects human IL-6 R in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human(rh) IL-1 alpha, recombinant mouse (rm) IL-1 alpha, rhIL-1 beta, rmIL-1 beta, rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.

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Applications and Data

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	0.25 µg/106 cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Human IL-6 R alpha Sandwich Immunoassay	Reagent
ELISA Capture (Matched Antibody Pair)	2-8 µg/mL	Human IL‑6 R alpha Antibody (Catalog #MAB227 )
ELISA Detection (Matched Antibody Pair)	0.1-0.4 µg/mL	Human IL‑6 R alpha Biotinylated Antibody (Catalog #BAF227 )
ELISA Standard		Recombinant Human IL-6 R alpha Protein (Catalog #227-SR )
Neutralization	Measured by its ability to neutralize IL‑6 R alpha -mediated enhancement of IL‑6-dependent Inhibition of cell proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 0.04-0.2 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑6 R alpha and 30 ng/mL Recombinant Human IL‑6.
Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

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Data Examples

Western Blot	click image to view larger	Detection of Recombinant Human IL‑6 R alpha by Western Blot. Western blot shows 25 ng of Recombinant Human IL‑6 R alpha (Catalog # 227-SR) and Recombinant Mouse IL‑6 R alpha (Catalog # 1830-SR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IL‑6 R alpha Monoclonal Antibody (Catalog # MAB227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL‑6 R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Flow Cytometry	click image to view larger	Detection of IL‑6 R alpha in U937 Human Cell Line by Flow Cytometry.U937 human histiocytic lymphoma cell line was labeled with Human IL‑6 R alpha Monoclonal Antibody (Catalog # MAB227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Neutralization	click image to view larger	IL‑6 R alpha Enhancement of IL‑6-dependent Inhibition of Cell Proliferation and Neutralization by Human IL‑6 R alpha Antibody.Recombinant Human IL‑6 R alpha (Catalog # 227-SR) enhances Recombinant Human IL‑6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL‑6 (30 ng/mL) activity elicited by Recombinant Human IL‑6 R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑6 R alpha Monoclonal Antibody (Catalog # MAB227). The ND50 is typically 0.04-0.2 µg/mL.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS.	Reconstitution Buffer Available
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-6 R alpha	The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4). A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

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References

Yamasaki et al. (1988) Science 241:825.
Baumann et al. (1990) J. Biol. Chem. 265:19853.
Hibi et al. (1990) Cell 63:1149.
Schooltink et al. (1991) Eur. J. Biochem. 277:659.
Novick et al. (1989) J. Exp. Med. 170:1409.
Yasukawa et al. (1990) J. Biochem. 108:673.
Saito et al. (1991) J. Immunology 147:168.
Long Name:	Interleukin 6 Receptor alpha
Entrez Gene IDs:	3570 (Human); 16194 (Mouse)
Alternate Names:	CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor

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Related Research Areas

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B Cell Activation
Cancer Stem Cell Markers
Cytokines and Receptors in Allergy and Asthma
Cytokines and Receptors in Angiogenesis
Cytokines and Receptors in Tfh Cell Differentiation and Function
Diabetes
IL-6 Family
Immune Regulatory Molecules on Endothelial Cells
Monocyte Markers
Multiple Sclerosis and Myelin Related Molecules
Myeloid-derived Suppressor Cells (MDSC)
Neuroinflammation
Obesity
Receptors in the Jak/STAT Pathway
T Cell Cytokine Signaling
Th17 Cells
Th22 Cells
IL-6 trans-signaling is another pathway to upregulate Osteopontin Cytokine, 2016;90(0):88-95. Species: Human Sample Type: Whole Cells Application: Flow Ctyometry
Images	Ratings	Applications	Species	Reviewed By	Date	Details
Enlarge	Excellent		ELISA	Human	Anonymous	05/31/2018			Image Details	ELISA: Human IL-6 R alpha Antibody [MAB227].

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Summary

Show

Application	ELISA
Sample Tested	EDTA Plasma
Species	Human
Other Experimental Details	Paired with BAF227 this antibody was used to measure IL-6 receptor in human plasma samples using a sandwich immunoassay.
	Excellent		WB	Human	Anonymous	11/29/2017

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Summary

Application	Western Blot
Sample Tested	Stimulated Human Bronchial Epithelial Cell Line (BEAS-2B)
Species	Human

Western Blot
	Detection of Recombinant Human IL‑6 R alpha by Western Blot. Western blot shows 25 ng of Recombinant Human IL‑6 R alpha (Catalog # 227-SR) and Recombinant Mouse IL‑6 R alpha (Catalog # 1830-SR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IL‑6 R alpha Monoclonal Antibody (Catalog # MAB227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL‑6 R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Flow Cytometry
	Detection of IL‑6 R alpha in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was labeled with Human IL‑6 R alpha Monoclonal Antibody (Catalog # MAB227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgGF(ab')2 Secondary Antibody (Catalog # F0102B).
Neutralization
	IL‑6 R alpha Enhancement ofIL‑6-dependent Inhibition of Cell Proliferation and Neutralization by HumanIL‑6 R alpha Antibody. Recombinant Human IL‑6 R alpha (Catalog #227-SR) enhances Recombinant Human IL‑6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL‑6 (30 ng/mL) activity elicited by Recombinant Human IL‑6 R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑6 R alpha Monoclonal Antibody (Catalog # MAB227). The ND50 is typically0.04-0.2 µg/mL.

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Related Product & Information

Long Name	Interleukin 6 Receptor alpha
Entrez Gene IDs	3570 (Human); 16194 (Mouse)
Background	IL-6 R alpha
background_content	Background: IL-6 R alpha The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

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Citations

1. IL-6 trans-signaling is another pathway to upregulate Osteopontin
   Cytokine, 2016;90(0):88-95.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow Ctyometry


2. Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium
   PLoS ONE, 2016;11(5):e0155161.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


3. Contact-dependent depletion of hydrogen peroxide by catalase is a novel mechanism of myeloid-derived suppressor cell induction operating in human hepatic stellate cells.
   Authors: Resheq Y, Li K, Ward S, Wilhelm A, Garg A, Curbishley S, Blahova M, Zimmermann H, Jitschin R, Mougiakakos D, Mackensen A, Weston C, Adams D
   J Immunol, 2015;194(6):2578-86.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


4. Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.
   Authors: Sommer A, Fabri M
   PLoS ONE, 2015;10(6):e0130395.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


5. Mutant B-RAF-Mcl-1 survival signaling depends on the STAT3 transcription factor.
   Authors: Becker, T M, Boyd, S C, Mijatov, B, Gowrishankar, K, Snoyman, S, Pupo, G M, Scolyer, R A, Mann, G J, Kefford, R F, Zhang, X D, Rizos, H
   Oncogene, 2014;33(9):1158-66.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


6. Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta.
   Authors: Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
   Nature, 2012;484(7395):514-8.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


7. Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
   Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E
   Nat. Med., 2011;17(11):1498-503.
   Species: Human
   Sample Type: In Vivo
   Application: In Vivo Homing


8. Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells.
   Authors: Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA
   Cancer Res., 2009;69(1):329-37.
   Species: Human
   Sample Type: Whole Cells
   Application: Flow


9. Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells.
   Authors: Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F
   Nat. Immunol., 2009;10(8):857-63.
   Species: Human
   Sample Type: Whole Cells
   Application: Neut


10. IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation.
    Authors: Kolliputi N, Waxman AB
    Am. J. Respir. Cell Mol. Biol., 2009;40(3):314-24.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB

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